miR-15b-5p targeting amyloid precursor protein is involved in the anti-amyloid eflect of curcumin in swAPP695-HEK293 cells

作     者：Hong-Ying Liu Xian Fu You-Fu Li Xian-Liang Li Zhen-Yu Ma Ying Zhang Qing-Chun Gao 

作者机构：Department of Neurology the Second Affiliated Hospital of Guangzhou Medical University 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2019年第14卷第9期

页      面：1603-1609页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 1010[医学-医学技术（可授医学、理学学位）] 100215[医学-康复医学与理疗学] 100204[医学-神经病学] 10[医学] 

基　　金：supported by the Science and Technology Planning Project of Guangdong Province of China,No.2016A020226022(to HYL) the Medical and Health Technology Project of Guangzhou of China,No.20161A011068(to HYL) the Guangzhou Science Technology and Innovation Commission of China,No.201704020043(to QCG) 

主　　题：nerve regeneration Alzheimer’s disease natural plant drug curcuminoids miRNAs amyloid precursor protein amyloid-β 3′-untranslated region Luciferase assays neurons neural regeneration 

摘      要：Curcumin exerts a neuroprotective effect on Alzheimer’s disease;however,it is not known whether microRNAs are involved in this protective *** study was conducted using swAPP695-HEK293 cells as an Alzheimer’s disease cell ***695-HEK293 cells were treated with 0,0.5,1,2,5,and 10μM curcumin for 24 *** changes in miR-15b-5p,miR-19a-3p,miR-195-5p,miR-101-3p,miR-216b-5p,miR-16-5p and miR-185-5p expression were assessed by real-time quantitative polymerase chain *** mRNA and protein levels of amyloid precursor protein,amyloid-β40 and amyloid-β42 were evaluated by quantitative real-time polymerase chain reaction,western blot assays and enzyme-linked immunosorbent ***695-HEK293 cells were transfected with miR-15b-5p mimic,or treated with 1μM curcumin 24 hours before miR-15b-5p inhibitor *** effects of curcumin on amyloid precursor protein,amyloid-β40 and amyloid-β42 levels were evaluated by western blot assays and enzyme-linked immunosorbent *** assays were used to analyze the interaction between miR-15b-5p and the 3′-untranslated region of amyloid precursor *** results show that amyloid precursor protein and amyloid-βexpression were enhanced in swAPP695-HEK293 cells compared with HEK293 parental *** suppressed the expression of amyloid precursor protein and amyloid-βand up-regulated the expression of miR-15b-5p in swAPP695-HEK293 *** addition,we found a negative association of miR-15b-5p expression with amyloid precursor protein and amyloid-βlevels in the curcumin-treated *** assays revealed that miR-15b-5p impaired the luciferase activity of the plasmid harboring the 3′-untranslated region of amyloid precursor *** findings indicate that curcumin down-regulates the expression of amyloid precursor protein and amyloid-βin swAPP695-HEK293 cells,which was partially mediated by miR-15b-5p via targeting of the 3′-untranslated region of amyloid precursor protein.