Predator stress-induced depression is associated with inhibition of hippocampal neurogenesis in adult male mice

作     者：Yan-Ping Wu Hua-Ying Gao Shu-Hua Ouyang Hiroshi Kurihara Rong-Rong He Yi-Fang Li 

作者机构：Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research College of Pharmacy Jinan University Anti-Stress and Health Research Center College of Pharmacy Jinan University 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2019年第14卷第2期

页      面：298-305页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 1010[医学-医学技术（可授医学、理学学位）] 100104[医学-病理学与病理生理学] 100215[医学-康复医学与理疗学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China,No.81473115(to RRH),81622050(to RRH) the Natural Science Foundation of Guangdong Province of China for Distinguished Young Scholars,No.2017A030306004(to YFL) the Youth Top-Notch Talent Support Program of Guangdong Province of China,No.2016TQ03R586(to YFL) 

主　　题：nerve regeneration depressive disorder cat stress cat odor stress behavioral evaluation open field test elevated plus maze test dark-avoidance test 5-hydroxytryptamine 5-hydroxyindoleacetic acid hippocampal neurogenesis neural regeneration 

摘      要：Stress has been suggested to disturb the 5-hydroxytryptamine system and decrease neurogenesis, which contribute to the development of depression. Few studies have investigated the effect of predator stress, a type of psychological stress, on depression and hippocampal neurogenesis in adult mice; we therefore investigated this in the present study. A total of 35 adult male Kunming mice were allocated to a cat stress group, cat odor stress group, cat stress + fluoxetine group, cat odor stress + fluoxetine group, or a control group(no stress/treatment). After 12 days of cat stress or cat odor stress, behavioral correlates of depression were measured using the open field test, elevated plus maze test, and dark-avoidance test. The concentrations of hippocampal 5-hydroxytryptamine and 5-hydroxyindoleacetic acid were measured using high-performance liquid chromatography-electrochemical detection. Neurogenesis was also analyzed using a bromodeoxyuridine and doublecortin double-immunostaining method. Cat stress and cat odor stress induced depression-like behaviors; this effect was stronger in the cat stress model. Furthermore, compared with the control group, cat stress mice exhibited lower 5-hydroxytryptamine concentrations, higher 5-hydroxyindoleacetic acid concentrations, and significantly fewer bromodeoxyuridine+/doublecortin+-labeled cells in the dentate gyrus, which was indicative of less neurogenesis. The changes observed in the cat stress group were not seen in the cat stress + fluoxetine group, which suggests that the effects of predator stress on depression and neurogenesis were reversed by fluoxetine. Taken together, our results indicate that depression-like behaviors induced by predator stress are associated with the inhibition of hippocampal neurogenesis.