An Aptamer-based Colorimetric Sensor for Streptomycin and Its Application in Food Inspection
An Aptamer-based Colorimetric Sensor for Streptomycin and Its Application in Food Inspection作者机构：College of Life Sciences Hebei University Baoding 071002 P. R. China Key Laboratory of Pharmaceutical Quality Control of Hebei Province Baoding 071002 P. R. China Office of Science and Technology Hebei University Baoding 071002 P. R. China
出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)
年 卷 期：2017年第33卷第5期
页 面：714-720页
核心收录：
学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 09[农学] 0903[农学-农业资源与环境]
基 金：Supported by the National Natural Science Foundation of China(No.81202338) the Natural Science Foundation of Hebei Province China(No.H2016201121) the National Training Program of Innovation and Entrepreneurship for Undergraduates China(No.201610075001) the Postdoctoral Science Foundation of China(No.2013M530885) and the Science and Technology Research Project of Hebei Higher Education Institutions China(No. ZD2017010)
主 题：Aptamer Streptomycin Systematic evolution of ligands by exponential enrichment(SELEX) Au nanopar- ticle Aptasensor
摘 要：An aptamer-based colorimetric biosensor was developed, which could be used to detect residual strepto- mycin from food quickly and cost-effectively. The ssDNA aptamer target for streptomycin was obtained using sys- temic evolution of ligands by exponential enrichment through affinity chromatography. A total of 19 candidates were obtained after 10 rounds of selection and were grouped into 3 families according to their similarity of sequence and structure. Among them, aptamer A15 showed the highest affinity for streptomycin tested by fluorescence intensity analysis with a dissociation constant of 6.07 nmol/L. Aptamer A15 also exhibited a higher streptomycin specificity with the lowest detectable limit of 25 nmol/L according to the value of A620/A520 ratio with AuNP-based colorimetric assay. The method was specific and sensitive for the detection of streptomycin from simply-treated milk and honey at 100 and 125 nmol/L, respectively, and is a promising approach to monitor antibiotics or other small molecules ana- logues in food inspection.
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