检索结果-南通市图书馆

aptamer-wrapped gold nanoparticles for the colorimetric detection of omethoate

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Chemistry 2016年第2期59卷 237-242页

作者： Pengjuan Wang Ying Wan Aldalbahi Ali Shengyuan Deng Yan Su Chunhai Fan Shulin Yang School of Mechanical Engineering Nanjing University of Science and Technology Nanjing 210094 China School of Envtronmental and Biologtcal and Engmeermg Nanjing University of Science and Technology Nanjing 210094 China Chemistry Department King Saud Universlty Riyadh 1145 Saudt Arabia Laboratory of Physical Biology Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 China

Organophosphorous pesticide(OP) contamination has serious adverse effects on human health and the environment. Due to the toxicity of OPs and the threat presented by their accidental or intentional release in populated areas, the determination and monitoring of these OPs in food products and environment is of great importance. OPs are present in very small quantities and therefore, methods for their detection need to be highly sensitive and selective. Here, we aimed to develop a simple and selective aptamer-based colorimetric assay for the detection of omethoate, which is one of the commonly used OPs. The principle of the assay is that single-stranded DNA(ss DNA)-wrapped gold nanoparticles(Au NPs) are resistant to salt-induced aggregation. By employing an "artificial antibody" organophosphorous pesticide-binding aptamer(OBA) as the recognition element, aptamer-wrapped Au NPs(Au-apta) show high selectivity towards omethoate, resulting in the disconnection of aptamers from Au NPs and the aggregation of Au NPs. As there is a significant color change from the interparticle plasmon coupling during the aggregation of Au NPs, the established assay showed good linearity between 0.1 and 10 μmol/L, with a low detection limit of 0.1 μmol/L. Other OPs such as profenofos, phorate, and isocarbophos would not interfere with the detection of omethoate despite having similar structures. Thus, the colorimetric method shows potential for use in the detection of omethoate in real soil samples.

关键词： aptamer DNA gold nanoparticles omethoate

aptamer-induced in-situ growth of acetylcholinesterase-Cu_(3)(PO_(4))_(2)hybrid nanoflowers for electrochemical detection of organophosphorus inhibitors

在线全文

学校读者我要写书评

暂无评论

Nano Research 2023年第10期16卷 12134-12143页

作者： Limin Yang Linjiao Qu Xiaolong Zhang Mingming Li Zhen Liu State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and Biotechnology China University of Petroleum(East China)Qingdao 266580China Institute of Biomedical Engineering Shenzhen Bay LaboratoryShenzhen 518055Chin

Enzyme-inorganic hybrid nanoflowers(HNFs)have shown excellent sensing capabilities due to their large specific surface area as well as the simplicity and mildness of the preparation ***,coupling HNFs to electrodes to fabricate a uniform and controllable enzymatic electrochemical sensing interface remains a ***,we proposed an aptamer-induced insitu fabrication strategy for preparing an HNF-based electrochemical sensor with ideal *** to this strategy is the introduction of acetylcholinesterase(AChE)-specific binding aptamer(Apt),which induces the in-situ growth of AChE-copper phosphate(Cu_(3)(PO_(4))_(2))HNFs on the surface of carbon paper(CP).In addition,a dense gold nanoparticle(AuNP)layer was electrodeposited on the CP for anchoring Apt and further extending the electroactive surface *** prepared AChECu_(3)(PO_(4))_(2)HNF/Apt/AuNP/CP biosensor exhibited a wide detection range from 1 to 107 pM for the four organophosphorus inhibitors,including isocarbophos,dichlorvos,methamidophos,and parathion,with detection limits down to 0.016,0.028,0.071,and 0.113 pM,*** the reactivation of pralidoxime chloride,the electrode can still recover 98.1%of the response after five times of repeated *** real sample detection,the biosensor achieved high recoveries from 96.45%to 100.13%.The detection target may be extendable to other AChE inhibitors(e.g.,drugs for Alzheimer’s disease).This study demonstrates for the first time the feasibility of using aptamers as an inducer to fabricate an electrochemical enzyme sensing interface *** strategy can be used to fabricate other enzyme-based biosensors and therefore has broader applications.

关键词： in-situ fabrication aptamer enzyme-inorganic hybrid nanoflowers electrochemical biosensor acetylcholinesterase inhibitors

aptamer-based molecular imaging

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Protein & Cell 2012年第10期3卷 739-754页

作者： Tianjiao Wang Judhajeet Ray Department of Biochemistry Biophysics and Molecular BiologyIowa State UniversityAmesIA 50011USA

Molecular imaging has greatly advanced basic biology and translational medicine through visualization and quantification of single/multiple molecular events temporally and spatially in a cellular context and in living ***,short single-stranded nucleic acids selected in vitro to bind a broad range of target molecules avidly and specifically,are ideal molecular recognition elements for probe development in molecular *** review summarizes the current state of aptamer-based biosensor development(probe design and imaging modalities)and their application in imaging small molecules,nucleic acids and proteins mostly in a cellular context with some animal *** article is concluded with a brief discussion on the perspective of aptamer-based molecular imaging.

关键词： aptamer imaging small molecule nucleic acid protein biosensor

Recent advances of aptamer sensors

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2008年第3期51卷 193-204页

作者： LI YiLin GUO Lei ZHANG ZhaoYang TANG JiJun XIE JianWei Beijing Institute of Pharmacology and Toxicology Beijing 100850China

aptamers are a series of high-affinity and high-specificity oligoneucleotides (single-stranded DNA or RNA) to the target, usually selected by the combinatorial chemistry SELEX technique (systematic evolution of ligands by exponential enrichment). aptamers have proved to be one kind of novel functional molecules in life science and chemistry. After being labeled by signaling groups, the aptamer probe can conveniently transfer the characteristics of aptamer-target recognition to a form of high-sensitive signal, and the high-affinity, high-specificity measurements of metal ion, organic molecules, nucleic acid, proteins, or cells become possible. This article summarizes the recent advances of aptamer probes in different sensing fields, with special emphasis on aptamer probes as fluorescent sensors.

关键词： SELEX aptamer aptazyme FRET cellular imaging

aptamer-Based Antibiotic Electrochemical Detection Platform Using Portable Plastic Gold Electrode and Multichannel Chip

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Chemistry 2024年第2期42卷 171-176页

作者： Yin Bao Kaiwei Cai Yichen Liu Bingling Li Changchun Institute of Applied Chemistry Chinese Academy of SciencesChangchunJilin 130022China School of Applied Chemistry and Engineering University of Science and Technology of ChinaHefeiAnhui 230026China

The development of accurate,rapid and practical electrochemical sensing technology for antibiotic detection has an important application prospect in many fields such as food safety,environmental monitoring,medical and health *** this research,kanamycin specific DNA aptamer and its partially complementary short chain were self-assembled onto the surface of an integrated portable plastic gold electrode(PGE)by Au-s bond,and a simple kanamycin electrochemical biosensor interface was *** the same time,the epoxy resin channel layer was designed,and the auxiliary instrument was set up independently in the laboratory,which could help to measure and analyze eight groups of samples automatically in *** quantitative analysis results showed that in 20 min,the sensor had a good linear relationship between the peak current change of square wave voltammetry and the negative logarithm of kanamycin concentration when kanamycin concentration was in the range of 1-10o0μmol/L,and the detection limit could reach 0.40μmol/*** addition,the discrimination could be achieved within 5 min in the real-time analysis *** sensor had a simple construction method,good selectivity and stability,and could be used for rapid or real-time detection of kanamycin residues in actual water samples,which provided a new direction for the practical detection of kanamycin in environmental watersamples.

关键词： Biosensor aptamer Antibiotics Electroanalysis DNA

aptamer-conjugated upconversion nanoprobes assisted by magnetic separation for effective isolation and sensitive detection of circulating tumor cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2014年第9期7卷 1327-1336页

作者： Shuai Fang Chao Wang Jian Xiang Liang Cheng Xuejiao Song Ligeng Xu Rui Peng Zhuang Liu Institute of Functional Nano & Soft Materials (FUNSOM) Collaborative Innovation Center of Suzhou Nano Science and TechnologySoochow University Suzhou Jiangsu 215123 China

Detection of circulating tumor cells （CTCs） plays an important role in cancer diagnosis and prognosis. In this study, aptamer-conjugated upconversion nano- particles （UCNPs） are used for the first time as nanoprobes to recognize tumor cells, which are then enriched by attaching with magnetic nanoparticles （MNPs） and placing in the presence of a magnetic field. Owing to the autofluorescence- free nature of upconversion luminescence imaging, as well as the use of magnetic separation to further reduce background signals, our technique allows for highly sensitive detection and collection of small numbers of tumor cells spiked into healthy blood samples, and shows promise for CTC detection in medical diagnostics.

关键词： circulating tumar cell (CTC)detection upconversion nanoparticles magnetic nanoparticles aptamer

aptamer-based and DNAzyme-linked colorimetric detection of cancer cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Protein & Cell 2010年第9期1卷 842-846页

作者： Xiaoli Zhu Ya Cao Zhiqiang Liang Genxi Li Laboratory of Biosensing Technology School of Life ScienceShanghai UniversityShanghai 200444China Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology Nanjing UniversityNanjing 210093China

This paper reports a novel method to detect human leukemic lymphoblasts(CCRF-CEM cells).While the aptamer of the cancer cells was employed as the recognition element to target cancer cells,peroxidaseactive DNAzyme was used as the sensing element to produce catalysis-induced colorimetric *** elegant architecture integrating the aptamer and DNAzyme made it feasible to detect cancer cells easily and rapidly by the color change of the substrate for *** results showed that 500 cells can well indicate the cancer,while as control,250,000 Islet Island Beta cells only show tiny signals,suggesting that the method proposed in this paper has considerable sensitivity and ***,since it does not require expensive apparatus,or modification or label of DNA chains,the method we present here is also costeffective and conveniently operated,implying potential applications in future cancer diagnosis.

关键词： CCRF-CEM acute leukemia cells aptamer DNAzyme colorimetry

aptamer-based Membrane Protein Analysis and Molecular Diagnostics

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2024年第2期40卷 173-189页

作者： ZHAO Long HU Haolan MA Xiaoqian LYU Yifan YUAN Quan TAN Weihong Molecular Science and Biomedicine Laboratory(MBL) State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of BiologyCollege of Chemistry and Chemical EngineeringAptamer Engineering Center of Hunan ProvinceHunan UniversityChangsha410082P.R.China The Cancer Hospital of the University of Chinese Academy of Sciences(Zhejiang Cancer Hospital) Hangzhou Institute of Medicine(HIM)Chinese Academy of SciencesHangzhou310022P.R.China Institute of Molecular Medicine(IMM) Renji HospitalSchool of MedicineCollege of Chemistry and Chemical EngineeringShanghai Jiao Tong UniversityShanghai200240P.R.China Furong Laboratory Changsha410082P.R.China

Membrane proteins are vital components of the cell membrane and play crucial roles in various cellular *** of membrane proteins is of paramount importance for studying molecular events inside cells and organisms and holds promising prospects for early disease diagnosis and treatment *** from obvious merits including high affinity,high specificity and ease of modification,aptamers have been regarded as ideal molecular recognition elements in membrane protein analysis and molecular diagnostics *** review summarised recent advances in membrane protein-specific aptamer screening,aptamer-based static and dynamic membrane protein analysis,and aptamer-based molecular diagnostic *** and challenges were also discussed.

关键词： Membrane protein aptamer DNA nanotechnology Bioanalysis Molecular diagnostics

aptamer structure switch fluorescence anisotropy assay for aflatoxin B1 using tetramethylrhodamine-guanine interaction to enhance signal change

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2020年第7期31卷 1982-1985页

作者： Yapiao Li Qiang Zhao State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China University of Chinese Academy of Sciences Beijing 100049China

Fluorescence anisotropy(FA)assay in homogenous solution is simple,sensitive and ***,we reported an aptamer structure switch FA assay for detection of aflatoxin B1(AFB1),one of the most toxic mycotoxins,by using tetra methylrhodamine(TMR)-labeled aptamer probe and its complementary DNA(cDNA)with tandem G bases extension,to meet the demand in sensitive and selective detection of AFB1,The hybridization of aptamer and cDNA drew TMR close to the repeated guanine(G)bases,and a high FA value was induced due to TMR-G inte raction and re stricted local rotation of *** the presence of AFB1,aptamer bound to AFB1 instead of the cDNA due to ***,the TMR-G interaction was eliminated,and FA value of TMR *** assay enabled the detection of AFB1 with detection limit of 125 pmol/L and dynamic range from 125 pmol/L to 31.2 nmol/L.

关键词： aptamer Aflatoxin Small molecule Fluorescence anisotropy Structure switch Fluorescence polarization