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Chemical Research in Chinese Universities 2024年第2期40卷 305-310页

作者： CHEN Yanyue LIU Jiali LIU Jiawen HU Rong YANG Yunhui ZHANG Xiaobing College of Chemistry and Chemical Engineering Yunnan Normal UniversityKunming650500P.R.China Molecular Science and Biomedicine Laboratory State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical EngineeringCollaborative Innovation Center for Molecular Engineering for TheronasticsHunan UniversityChangsha410082P.R.China

The insufficient internal resorption of iodide ions(I−)leads to thyroid disorders,such as goiter,hypothyroidism,and cretinism in adults.In this paper,a portable point-of-care testing(POCT)platform was developed for the dual-modal analysis of I^(−),seamlessly integrating both colorimetric and photothermal thermometer techniques.The quantification of results was achieved using a standard thermometer or smartphone.G-Quadruplex/Hemin(G4/Hemin)is the DNAzyme with peroxidase-like activity.The catalytic efficacy of G-quadruplex structures can be enhanced with the help of I^(−),manifesting as multicolor transitions from colorless to green and then blue,and accompanied by the increase of temperature,which can be used for the quantitative detection of I^(−).Additionally,digital analysis according to the three-color channels(R/G/B)by a cellphone eliminated the requirement for intricate instruments.This dual-modal method for portable I^(−)determination is cost-effectiveness,simplicity,remarkable sensitivity(0.5 nmol/L)and selectivity.Besides,it was applied to determining the concentration of I^(−)in spiked serum samples.

关键词： Dual-modal platform G-Quadruplex Iodide ion Point-of-care testing(POCT) aptasensor

Mobile mini-fluorimeter for antibiotic aptasensing based on surface-plasmonic effect of burlike nanogolds enhanced by digitized imaging diagnosis

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Chinese Chemical Letters 2024年第5期35卷 418-422页

作者： Tiantian Man Fulin Zhu Yaqi Huang Yuhao Piao Yan Su Shengyuan Deng Ying Wan School of Mechanical Engineering Nanjing University of Science and TechnologyNanjing 210094China Key Laboratory of Metabolic Engineering and Biosynthesis Technology Ministry of Industry and Information TechnologySchool of Environmental and Biological EngineeringNanjing University of Science and TechnologyNanjing 210094China

Antibiotic abuse now poses a grave threat to global ecology and bestirs public concerns about the residue issue in daily necessities.The traceability measurements along supply chain or logistic circulation have become increasingly essential given the labile nature of diverse synthetic residuals on site.In an attempt to answer this urgency,here a miniaturized fluorometric aptasensor prototype was contrived that catered to the point-of-care screening norm for two typical additives:chloramphenicol and enrofloxacin.The key target-indicating module worked in vitro based on the competitive binding-induced fluorescence recovery of fluorescein-labeled aptamers,which were photobleached beforehand in the format of double helix on burlike nanogold carriers.The“prickly”geometry of the latter not just enriched the capture probes at preferentially substrate-accessible spires;but also contributed to a tip-enhanced surface plasmon effect,sensitizing the signal-on during the duplex dissociation even at nanomolar threshold of the analytes.On the other hand,to encompass a full portable,a set of optical devices were mounted within a 3D-printed cartridge(adaptor)to converge the light beam and route it towards the detector,for which the smartphone camera came up in handy with a home-developed App for calibrating the emissive brightness.Enlightened by the high-dynamic-range compression,an imaging diagnostic algorithm was built in to grid and digitize each slide in the album for augmented detection performance.Thus,a novel bio-to-silico integration was invented that capable of in situ rapid reporting on the antibiotic presence with high sensitivity and selectivity.Further field practices in spiked milk on sales proved the precision and rudimentary feasibility of the well-assembled model of appliance,thus holding nice prospects in nonexpert(e.g.,family and local community)utilities for foodborne antibiotic identification.

关键词： Chloramphenicol and enrofloxacin aptasensor Gold nanoburs Smartphone fluorimetric suite Digitized image postprocessing

aptasensor Based on Screen-Printed Carbon Electrodes Modified with CS/AuNPs for Sensitive Detection of Okadaic Acid in Shellfish

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Journal of Analysis and Testing 2023年第2期7卷 128-135页

作者： Ni Zeng Xinyi Wang Yiyang Dong Yan Yang Yingai Yin Lianhui Zhao Xu Wang College of Life Science and Technology Beijing University of Chemical TechnologyBeijing 100029China

Okadaic acid(OA),a small molecule substance derived from shellfish,is one of the most widely distributed marine toxins with acute symptoms of vomiting and diarrhea after accidental ingestion.For this,there is an urgently need for sensitive and reliable methods to detect OA in real shellfish samples.In this study,a simple aptasensor based on screen-printed carbon electrode(SPCE)with modification of chitosan(CS)and gold nanoparticles(Au NPs)was designed for electrochemical determination of OA,and the electrode surface was modified with Au NPs by potential-sweeping electrodeposition,which greatly improved the electrochemical response.The entire detection and characterization process were carried out by cyclic voltammetry(CV)with a linear correlation in the range of 0.01-100 ng/m L and a limit of detection(LOD)of 6.7 pg/m L.Furthermore,recovery rates of 92.3-116%were obtained demonstrating excellent accuracy through the recovery trial of mussel and scallop samples.

关键词： Okadaic acid aptasensor Screen-printed carbon electrode Chitosan Gold nanoparticles Cyclic voltammetry

Fluorescent aptasensor for detection of live foodborne pathogens based on multicolor perovskite-quantum-dot-encoded DNA probes and dual-stirring-bar-assisted signal amplification

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Journal of Pharmaceutical Analysis 2022年第6期12卷 913-922页

作者： Liu Liu Juncheng Hong Wenhai Wang Shu Xiao Hongzhen Xie Qiqin Wang Ning Gan School of Material Science and Chemical Engineering Ningbo UniversityNingboZhejiang315211China Institute of Pharmaceutical Analysis College of PharmacyJinan UniversityGuangzhou510632China

In this study,a fluorescent(FL)aptasensor was developed for on-site detection of live Salmonella typhimurium(S.T.)and Vibrio parahaemolyticus(V.P.).Complementary DNA(cDNA)of aptamer(Apt)-functionalized multicolor polyhedral oligomeric silsesquioxane-perovskite quantum dots(cDNA-POSSPQDs)were used as encoded probes and combined with dual-stirring-bar-assisted signal amplification for pathogen quantification.In this system,bar 1 was labeled with the S.T.and V.P.Apts,and then bar 2 was functionalized with cDNA-POSS-PQDs.When S.T.and V.P.were introduced,pathogen-Apt complexes would form and be released into the supernatant from bar 1.Under agitation,the two complexes reached bar 2 and subsequently reacted with cDNA-POSS-PQDs,which were immobilized on MXene.Then,the encoded probes would be detached from bar 2 to generate FL signals in the supernatant.Notably,the pathogens can resume their free state and initiate next cycle.They swim between the two bars,and the FL signals can be gradually enhanced to maximum after several cycles.The FL signals from released encoded probes can be used to detect the analytes.In particular,live pathogens can be distinguished from dead ones by using an assay.The detection limits and linear range for S.T.and V.P.were 30 and 10 CFU/mL and 10^(2) -10^(6) CFU/mL,respectively.Therefore,this assay has broad application potential for simultaneous on-site detection of various live pathogenic bacteria in water.

关键词： aptasensor Live bacteria Multiple detection Multicolor perovskite quantum dots Dual-stirring-bar

An antifouling polydopamine-based fluorescent aptasensor for determination of arginine kinase

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Food Science and Human Wellness 2023年第3期12卷 737-744页

作者： Yanbo Wang Huan Li Jinru Zhou Fangting Wang Yifan Qian Linglin Fu Food Safety Key Laboratory of Zhejiang Province School of Food Science and BiotechnologyZhejiang Gongshang UniversityHangzhou 310018China

Simple yet efficient detection methods for food allergens are in urgent need to help people avoid the risks imposed by allergenic food.In this work,a polydopamine(PDA)-based fluorescent aptasensor was developed to detect arginine kinase(AK),one of the major allergens in shellfish.The aptamer towards AK was firstly selected via systematic evolution of ligands by exponential enrichment method and labeled with fluorescein amidite(FAM)to build a fluorescence resonance energy transfer(FRET)system with PDA particles.Polyethylene glycol(PEG)was employed to construct an antifouling surface for the aptasensor to eliminate food matrix interferences.With the presence of AK,the PDA-based aptasensor exhibited elevated fluorescent signals as the FAM-labeled aptamer bound to AK and detached from the PDA particles.The aptasensor showed great stability and resistance to nonspecific interference of background proteins and had a limit of detection(LOD)of 0.298μg/mL.The proposed aptasensor was further proved to be feasible for quantitative analysis of AK in nine species of shrimps and five commercial processed products,which indicated its high potential in tracing the presence of AK in complex aquatic products.

关键词： Food allergy aptasensor Antifouling Polydopamine FRET effect

A Label-free Fluorescent aptasensor for Turn-on Monitoring Ochratoxin A Based on AIE-active Probe and Graphene Oxide

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Chemical Research in Chinese Universities 2018年第3期34卷 363-368页

作者： MA Lian XU Bin LIU Leijing TIAN Wenjing State Key Laboratory of Supramolecular Structure and Materials Jilin University Changehun 130012 P. R. China

A label-free fluorescent aptasensor for specific and ultrasensitive monitoring ochratoxin A（OTA） was developed using the specific aptamer of OTA（OSA） as recognition dement, an aggregation-induced emission（AIE） molecule（a 9,10-distyrylanthracene with two ammonium groups, DSAI） as a fluorescent probe, and graphene oxide（GO） as a quencher. In the absence of OTA, the AIE probe DSAI and OSA complex（DSAI/OSA） is adsorbed on the GO surface, and the fluorescence of DSAI will be quenched efficiently via the fluorescence resonance energy transfer（FRET） from DSAI to GO. Upon the OTA addition, a more stable complex（OSA-OTA） is formed and released from GO. Meanwhile, DSAI and OSA-OTA can form a new complex（DSAI/OSA-OTA）, then the fluorescent signal of DSAI recovers gradually. Therefore, by introducing GO and DSAI, the fluorescence signal of DSAI can be easily turned from ＂off＂ to ＂on＂ after the addition of OTA, and the ultrasensitive detection of OTA by monitoring the change of the fluorescence signal of DSAI can be readily realized. The detection limit of the assay can reach 0.324 nmol/L with a linear detection range of 10-200 nmol/L. And the aptasensor exhibits high selectivity for OTA against other analogues. Moreover, it has been successfully applied for the detection of OTA in red wine samples.

关键词： Label-free aptasensor Aggregation-induced emission（AIE）-active probe Graphene oxide Ochratoxin A

Electrochemical aptasensor Based on Prussian Blue-Chitosan-Glutaraldehyde for the Sensitive Determination of Tetracycline

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Nano-Micro Letters 2014年第2期6卷 143-152页

作者： Guanghui Shen Yemin Guo Xia Sun Xiangyou Wang School of Agricultural and Food Engineering Shandong University of Technology

In this paper, a novel and sensitive electrochemical aptasensor for detecting tetracycline(TET)with prussian blue(PB) as the label-free signal was fabricated. A PB-chitosan-glutaraldehyde(PB-CS-GA)system acting as the signal indicator was developed to improve the sensitivity of the electrochemical aptasensor.Firstly, the PB-CS-GA was fixed onto the glass carbon electrode surface. Then, colloidal gold nanoparticles(Au NPs) were droped onto the electrode to immobilize the anti-TET aptamer for preparation of the aptasensor.The stepwise assembly process of the aptasensor was characterized by cyclic voltammetry(C-V) and scanning electron microscope(SEM). The target TET captured onto the electrode induced the current response of the electrode due to the non-conducting biomoleculars. Under the optimum operating conditions, the response of differential pulse voltammetry(DPV) was used for detecting the concentration of TET. The proposed aptasensor showed a high sensitivity and a wide linear range of 109-～ 105-M and 105-～ 102-M with the correlation coefficients of 0.994 and 0.992, respectively. The detection limit was 3.2×1010-M(RSD 4.12%). Due to its rapidity, sensitivity and low cost, the proposed aptasensor could be used as a pre-scanning method in TET determination for the analysis of livestock products.

关键词： aptasensor Tetracycline Colloidal gold nanoparticle Chitosan

Ultrasensitive and Signal-on Electrochemiluminescence aptasensor Using the Multi-tris(bipyridine)ruthenium(Ⅱ)- β-cyclodextrin Complexes

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Chinese Journal of Chemistry 2014年第11期32卷 1161-1168页

作者： Yingying Zhao Hong Chen Qiong Chen Yantao Qi Fan Yang Jie Tang Pingang He Fan Zhang Department of Chemistry East China Normal UniversityShanghai 200241China

An ultrasensitive and signal-on electrochemiluminescence(ECL)aptasensor to detect target protein(thrombin or lysozyme)was developed using the host-guest recognition between a metallocyclodextrin complex and single-stranded DNA(ss-DNA).The aptasensor uses both the photoactive properties of the metallocyclodextrins named multi-tris(bipyridine)ruthenium(Ⅱ)-β-cyclodextrin complexes and their specific recognition with ss-DNA,which amplified the ECL signal without luminophore labeling.After investigating the ECL performance of different multi-tris(bipyridine)ruthenium(Ⅱ)-β-cyclodextrin(multi-Ru-β-CD)complexes,tris-tris(bipyridine)-ruthenium(Ⅱ)-β-cyclodextrin(tris(bpyRu)-β-CD)was selected as a suitable host molecule to construct an atasensor.First,double-stranded DNA(ds-DNA)formed by hybridization of the aptamer and its target DNA was attached to a glassy carbon electrode via coupling interaction,which showed low ECL intensity with 2-(dibutylamino)ethanol(DBAE)as coreactant,because of the weak recognition between ds-DNA and tris(bpyRu)-β-CD.Upon addition of the corresponding protein,the ECL intensity increased when target ss-DNA was released because of the higher stability of the aptamer-protein complex than the aptamer-DNA one.A linear relationship was observed in the range of 0.01 pmol/L to 100 pmol/L between ECL intensity and the logarithm of thrombin concentrations with a limited detection of 8.5 fmol/L(S/N=3).Meanwhile,the measured concentration of lysozyme was from 0.05 pmol/L to 500 pmol/L and the detection limit was 33 fmol/L(S/N=3).The investigations of proteins in human serum samples were also performed to demonstrate the validity of detection in real clinical samples.The simplicity,high sensitivity and specificity of this aptasensor show great promise for practical applications in protein monitoring and disease diagnosis.

关键词： aptasensor host-guest recognition electrochemiluminescence metallocyclodextrin

A novel aptasensor strategy for protein detection based on G-quadruplex and exonuclease Ⅲ-aided recycling amplification

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Chinese Chemical Letters 2020年第1期31卷 155-158页

作者： Huan Shi Tian Jin Jiewen Zhang Xiaoting Huang Chunyan Tan Yuyang Jiang Ying Tan State Key Laboratory of Chemical Oncogenomics Key Laboratory of Chemical Biologythe Graduate School at ShenzhenTsinghua UniversityShenzhen 518055China Department of Chemistry Tsinghua UniversityBeijing 100084China Department of Pharmacology and Pharmaceutical Sciences School of MedicineTsinghua UniversityBeijing 100084China

The detection of biomarkers is of great significance in the diagnosis of numerous diseases,especially cancer.Herein,we developed a sensitive and universal fluorescent aptasensor strategy based on magnetic beads,DNA G-quadruplex,and exonuclease Ⅲ(Exo Ⅲ).In the presence of a target protein,a label-free single strand DNA(ssDNA)hybridized with the aptamer was released as a trigger DNA due to specific recognition between the aptamer and target.Subsequently,ssDNA initiates the ExoⅢ-aided recycling to amplify the fluorescence signal,which was caused by N-methylmesoporphyrin IX(NMM)insertion into the G-quadruplex structure.This proposed strategy combines the excellent specificity between the aptamer and target,high sensitivity of the fluorescence signal by G-quadruplex and ExoⅢ-aided recycling amplification.We selected(50-1200 nmol/L)MUC1,a common tumor biomarker,as the proof-of-concept target to test the specificity of our aptasenso r.Results reveal that the sensor sensitively and selectively detected the target protein with limits of detection(LODs)of 3.68 and 12.83 nmol/L in buffer solution and 10%serum system,respectively.The strategy can be easily applied to other targets by simply substituting corresponding aptamers and has great potential in the diagnosis and monitoring of several diseases.

关键词： aptasensor ExonucleaseⅢ-aided recycling amplification G-quadruplex Magnetic beads MUC1

Handheld aptasensor for Sandwiched Detection of Chloramphenicol

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Chemical Research in Chinese Universities 2020年第2期36卷 291-295页

作者： YU Mintong XIA Tian BAI Wanchen JI jinyu WANG Huan HUANG Yaqi DENG Shengyuan MA Kefeng SU Yan WAN Ying School of Mechanical Engineering Nanjing University of Science and TechnologyNanjing 210094P.R.China Key Laboratory of New Membrane Materials Ministry of Industry and Information TechnologySchool of Environmental and Biological EngineeringNanjing University of Science and Technology/Nanjing 210094R R.China

Chloramphenicol(CAP)is an antifungal agent approved for use in aquarium.However,CAP residue imparts serious adverse effect on human health as well as the ecology.Thus,keeping track of CAP in aquatic products and its environmental metabolites are of great importance.Herein,we developed a novel and neat electrochemical aptasensor for the detection of simulated CAP residual in a sandwich fashion.The screen-printed carbon electrode was employed as the sensing platform and electrodeposited with peculiar nanogolds to enhance the electrochemical performance.For the specific recognition of such small target,a pair of chloramphenicol-binding aptamers,the primary Aptl and the secondary Apt2,were tailored as an artificial single-chained antibody couple,of which the thi-olated Aptl stood on the coated working substrate to capture the analyte,while the biotinylated Apt2 rallied the signal output.In the presence of CAP,Aptl at the lower place cooperated with the upper Apt2 pinched CAP together.After that,the reporter avidin-conjugated horseradish peroxidase associated atop the mounted biotin labels,resulting in an electrocatalytic amplification.In whole,this modular biosensing construction would set a paradigm not just for practicable checkpoint quarantine or point-of-care testing,but potentially adaptable for technological fusion with“Internet Plus”and“Internet of Things”.

关键词： Chloramphenicol Screen-printed carbon electrode Electrochemical aptasensor Electrochemical deposi tion Ufkimm