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BCI induces apoptosis via generation of reactive oxygen species and activation of intrinsic mitochondrial pathway in H1299 lung cancer cells

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Science China(Life Sciences) 2018年第10期61卷 1243-1253页

作者： Jong-Woon Shin Sae-Bom Kwon Yesol Bak Sang-Ku Lee Do-Young Yoon Department of bioscience and Biotechnology Konkuk University Targeted Medicine research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB)

The compound(E)-2-benzylidene-3-(cyclohexylamino)-2,3-dihydro-1 H-inden-1-one(BCI) is known as an inhibitor of dual specific phosphatase 1/6 and mitogen-activated protein kinase. However, its precise anti-lung cancer mechanism remains unknown. In this study, the effects of BCI on the viability of non-small cell lung cancer cell lines NCI-H1299, A549, and NCI-H460 were evaluated. We confirmed that BCI significantly inhibited the viability of p53(-) NCI-H1299 cells as compared to NCI-H460 and A549 cells, which express wild-type p53. Furthermore, BCI treatment increased the level of cellular reactive oxygen species and pre-treatment of cells with N-acetylcysteine markedly attenuated BCI-mediated apoptosis of NCI-H1299 cells. BCI induced cellular morphological changes, inhibited viability, and produced reactive oxygen species in NCI-H1299 cells in a dose-dependent manner. BCI induced processing of caspase-9, caspase-3, and poly ADP-ribose polymerase as well as the release of cytochrome c from the mitochondria into the cytosol. In addition, BCI downregulated Bcl-2 expression and enhanced Bax expression in a dose-dependent manner in NCI-H1299 cells. However, BCI failed to modulate the expression of the death receptor and extrinsic factor caspase-8 and Bid, a linker between the intrinsic and extrinsic apoptotic pathways in NCI-H1299 cells. Thus, BCI induces apoptosis via generation of reactive oxygen species and activation of the intrinsic pathway in NCI-H1299 cells.

关键词： non-small cell lung cancer apoptosis BCI cytotoxicity anticancer ROS

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Chromosome-level genome and population genomics of the intermediate horseshoe bat(Rhinolophus affinis)reveal the molecular basis of virus tolerance in Rhinolophus and echolocation call frequency variation

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Zoological research 2024年第5期45卷 1147-1160页

作者： Le Zhao Jiaqing Yuan Guiqiang Wang Haohao Jing Chen Huang Lulu Xu Xiao Xu Ting Sun Wu Chen Xiuguang Mao Gang Li College of Life Sciences Shaanxi Normal UniversityXi’anShaanxi 710119China QinLing-Bashan Mountains Bioresources Comprehensive Development C.I.C. School of Bioscience and EngineeringShaanxi University of TechnologyHanzhongShaanxi 723001China Guangzhou Zoo GuangzhouGuangdong 510070China School of Ecological and Environmental Sciences East China Normal UniversityShanghai 200241China

Horseshoe bats(genus Rhinolophus,family Rhinolophidae)represent an important group within chiropteran phylogeny due to their distinctive traits,including constant high-frequency echolocation,rapid karyotype evolution,and unique immune *** in evolutionary biology,supported by high-quality reference genomes and comprehensive whole-genome data,have significantly enhanced our understanding of species origins,speciation mechanisms,adaptive evolutionary processes,and phenotypic ***,genomic research and understanding of the evolutionary patterns of Rhinolophus are severely constrained by limited data,with only a single published genome of *** currently *** this study,we constructed a high-quality chromosome-level reference genome for the intermediate horseshoe bat(***).Comparative genomic analyses revealed potential genetic characteristics associated with virus tolerance in ***,we observed expansions in several immune-related gene families and identified various genes functionally associated with the Sars-CoV-2 signaling pathway,DNA repair,and apoptosis,which displayed signs of rapid *** addition,we observed an expansion of the major histocompatibility complex class II(MHC-II)region and a higher copy number of the HLA-DQB2 gene in horseshoe bats compared to other chiropteran *** on whole-genome resequencing and population genomic analyses,we identified multiple candidate loci(e.g.,GLI3)associated with variations in echolocation call frequency across *** *** research not only expands our understanding of the genetic characteristics of the Rhinolophus genus but also establishes a valuable foundation for future research.

关键词： Reference-quality genome Comparative genomics Population genomics Positive selection Bats

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Poly(ferulic acid)nanocarrier enhances chemotherapy sensitivity of acute myeloid leukemia by selectively targeting inflammatory macrophages

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Chinese Chemical Letters 2024年第9期35卷 289-295页

作者： Weijian Zhang Xianyu Deng Liying Wang Jian Wang Xiuting Guo Lianggui Huang Xinyi Wang Jun Wu Linjia Jiang Guangdong Provincial Key laboratory of Malignant Tumor Epigenetics and Gene Regulation Sun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhou 510120China Department of Otolaryngology Sun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhou 510120China Department of Hematology The Seventh Affiliated HospitalSun Yat-sen UniversityShenzhen 518107China Department of Pediatrics Sun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhou 510120China bioscience and Biomedical Engineering Thrust The Hong Kong University of Science and Technology(Guangzhou)NanshaGuangzhou 511400China Division of Life Science The Hong Kong University of Science and TechnologyHong Kong SAR 999077China

Macrophages,as a subset of innate immune cells,play a pivotal role in the initiation,maintenance,and resolution of inflammatory responses during tissue damage repair,defense against infections,and tumor ***,the mechanisms by which macrophages regulate inflammation in acute myeloid leukemia(AML)and their involvement in the chemotherapeutic effect remain *** this study,we have identified that AML cells stimulate macrophage expansion by activating the colony-stimulating factor 1 receptor(CSF1R)*** expanded macrophages activate nuclear factor kappa-B(NFκB)to induce the expression of inflammatory factors,thereby maintaining leukemic cell quiescence and promoting cell survival following ***,we have successfully utilized a poly(ferulic acid)nanocarrier to selectively target macrophages for inhibiting the NFκB-mediated inflammation,ultimately enhancing chemotherapy efficacy against *** together,our findings highlight the crucial role of macrophage-induced inflammation in conferring chemoresistance to AML,and demonstrate the potential of a targeted nanocarrier specifically designed for inflammatory macrophages to improve the AML chemotherapeutic outcomes.

关键词： Acute myeloid leukemia Chemoresistance Macrophage Inflammation NFκB

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Mitochondrial Ca^(2+) uptake in skeletal muscle health and disease

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Science China(Life Sciences) 2016年第8期59卷 770-776页

作者： Jingsong Zhou Kamal Dhakal Jianxun Yi Kansas City University of Medicine and bioscience Dybedal Research Center

Muscle uses Ca2＋ as a messenger to control contraction and relies on ATP to maintain the intracellular Ca2＋ homeostasis. Mi- tochondria are the major sub-cellular organelle of ATP production. With a negative inner membrane potential, mitochondria take up Ca2＋ from their surroundings, a process called mitochondrial Ca2＋ uptake. Under physiological conditions, Ca2＋ uptake into mitochondria promotes ATP production. Excessive uptake causes mitochondrial Ca2＋ overload, which activates down- stream adverse responses leading to cell dysfunction. Moreover, mitochondrial Ca2＋ uptake could shape spatio-temporal pat- terns of intracellular Ca〉 signaling. Malfunction of mitochondrial Ca2＋ uptake is implicated in muscle degeneration. Unlike non-excitable cells, mitochondria in muscle cells experience dramatic changes of intracellular Ca2＋ levels. Besides the sudden elevation of Ca2＋ level induced by action potentials, Ca2＋ transients in muscle cells can be as short as a few milliseconds during a single twitch or as long as minutes during tetanic contraction, which raises the question whether mitochondrial Ca2＋ uptake is fast and big enough to shape intracellular Ca2＋ signaling during excitation-contraction coupling and creates technical challeng- es for quantification of the dynamic changes of Ca2＋ inside mitochondria. This review focuses on characterization of mito- chondrial Ca2＋ uptake in skeletal muscle and its role in muscle physiology and diseases.

关键词： skeletal muscle mitochondria Ca2+

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Protein trafficking during plant innate immunity

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Journal of Integrative Plant Biology 2016年第4期58卷 284-298页

作者： Wen-Ming Wang Peng-Qiang Liu Yong-Ju Xu Shunyuan Xiao Rice research Institute & Key laboratory for Major Crop Diseases Sichuan Agricultural University Institute for bioscience and Biotechnology research & Department of Plant Science and Landscape Architecture University of Maryland

Plants have evolved a sophisticated immune system to fight against pathogenic microbes. Upon detection of pathogen invasion by immune receptors, the immune system is turned on, resulting in production of antimicrobial molecules including pathogenesis-related（PR） ***, an efficient immune response depends on the capacity of the plant cell＇s protein/membrane trafficking network to deploy the right defense-associated molecules in the right place at the right time. Recent research in this area shows that while the abundance of cell surface immune receptors is regulated by endocytosis, many intracellular immune receptors, when activated, are partitioned between the cytoplasm and the nucleus for induction of defense genes and activation of programmed cell death, respectively. Vesicle transport is an essential process for secretion of PR proteins to the apoplastic space and targeting of defense-related proteins to the plasma membrane or other endomembrane compartments. In this review, we discuss the various aspects of protein trafficking during plant immunity, with a focus on the immunity proteins on the move and the major components of the trafficking machineries engaged.

关键词： Endocytic trafficking nucleocytoplasmic partitioning programmed cell death small GTPase SNARE vesicle transport

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Oxidative stress and antioxidants in hepatic pathogenesis

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World Journal of Gastroenterology 2010年第48期16卷 6035-6043页

作者： Hye-Lin Ha Hye-Jun Shin Mark A Feitelson Dae-Yeul Yu Disease Model research laboratory Aging Research CenterKorea Research Institute of Bioscience and Biotechnology Department of Functional Genomics University of Science and Technology Department of Biology College of Science and TechnologyTemple University

Long term hepatitis B virus (HBV) infection is a major risk factor in pathogenesis of chronic liver diseases,including hepatocellular carcinoma (HCC). The HBV encod-ed proteins,hepatitis B virus X protein and preS,appear to contribute importantly to the pathogenesis of HCC. Both are associated with oxidative stress,which can damage cellular molecules like lipids,proteins,and DNA during chronic infection. Chronic alcohol use is another important factor that contributes to oxidative stress in the liver. Previous studies reported that treatment with antioxidants,such as curcumin,silymarin,green tea,and vitamins C and E,can protect DNA from damage and regulate liver pathogenesis-related cascades by reducing reactive oxygen species. This review summarizes some of the relationships between oxidative stress and liver pathogenesis,focusing upon HBV and alcohol,and suggests antioxidant therapeutic approaches.

关键词： Hepatitis B virus Hepatitis B virus X protein Alcohol Chronic liver disease Oxidative stress Antioxidant

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Success of three soil restoration techniques on seedling survival and growth of three plant species in the Sahel of Burkina Faso(West Africa)

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Journal of Forestry research 2016年第2期27卷 313-320页

作者： Philippe Bayen Anne Mette Lykke Adjima Thiombiano laboratory of Plant Biology and Ecology University of Ouagadougou Department of bioscience Aarhus University

In the Sahel of Africa, where 90 % of the population depends on natural resources for their liveli- hood, a large part of the soils are structurally unstable, prone to crusting and hard setting, and have low water holding capacity, which hamper vegetation establishment. The effect of soil restoration techniques on survival and growth of seedlings of Acacia nilotica, Acacia tortilis and Jatropha curcas was tested in completely barren, degraded land in a Sahelian ecosystem in Burkina Faso. A total of 522 seedlings （174 plants of each plant species） were planted in a randomized complete block design with three replicates combining three soil preparation techniques： half-moon, zai＂ and standard plantation. Survival and growth rates evaluated over 20 months were significantly higher using the half-moon technique compared with the other two techniques. Survival rates of plant species planted using half-moon technique were 62.5, 28.57 and 10.71% for A. nilotica, A. tortilis and J. curcas respec- tively, but in za＇i and standard planting, seedling survival was zero. The low survival rate of J. curcas using the half-moon technique may indicate that J. curcas is unsuit- able for barren and degraded land, whereas A. nilotica and A. tortilis appear to be promising tree species for rehabil- itation of degraded land.

关键词： Afforestation Bare soil Soil rehabilitation Species selection Water conservation

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On-chip physiological mimicry of neurovascular unit: challenges and perspectives

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Neural Regeneration research 2024年第3期19卷 499-500页

作者： Song Ih Ahn YongTae Kim School of Mechanical Engineering Pusan National UniversityBusanSouth Korea George W.Woodruff School of Mechanical Engineering Georgia Institute of TechnologyAtlantaGAUSA Parker H.Petit Institute for Bioengineering and bioscience Georgia Institute of TechnologyAtlantaGAUSA Wallace H.Coulter Department of Biomedical Engineering Georgia Institute of TechnologyAtlantaGAUSA Institute for Electronics and Nanotechnology Georgia Institute of TechnologyAtlantaGAUSA

Neurological disorders including neurodegenerative diseases,brain tumors,and stroke are the second leading cause of death and the greatest cause of disability ***,it remains challenging to achieve effective drug delivery to the central nervous system for treatments of neurological diseases due to the blood-brain barrier(BBB).The function of the BBB is regulated by the physiological interactions between various types of cells in the neurovascular unit(NVU).In the NVU,the brain vasculature of the BBB is surrounded by brain pericytes,brain astrocytes,neurons,and microglia(Figure 1).Moreover,the NVU at the levels of arteries and veins includes contractile smooth muscle cells(Schaeffer and Iadecola,2021).

关键词： diseases death arteries

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Half a century after their discovery:Structural insights into exonuclease and annealase proteins catalyzing recombineering

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Engineering Microbiology 2024年第1期4卷 39-54页

作者： Lucy J.Fitschen Timothy P.Newing Nikolas P.Johnston Charles E.Bell Gökhan Tolun School of Chemistry and Molecular bioscience and Molecular HorizonsUniversity of WollongongWollongongNSWAustralia The ARC Training Centre for Cryo-electron Microscopy of Membrane Proteins University of WollongongWollongongNSWAustralia Faculty of Science University of Technology SydneyUltimoNSW 2007Australia Department of Biological Chemistry and Pharmacology The Ohio State University College of MedicineColumbusOH 43210

Recombineering is an essential tool for molecular biologists,allowing for the facile and efficient manipulation of bacterial genomes directly in cells without the need for costly and laborious in vitro manipulations involving restriction *** main workhorses behind recombineering are bacteriophage proteins that promote the single-strand annealing(SSA)homologous recombination pathway to repair double-stranded DNA *** there have been several reviews examining recombineering methods and applications,comparatively few have focused on the mechanisms of the proteins that are the key players in the SSA pathway:a 5′→3′exonuclease and a single-strand annealing protein(SSAP or“annealase”).This review dives into the structures and functions of the two SSA recombination systems that were the first to be developed for recombineering in ***:the RecET system from *** Rac prophage and the𝜆Red system from bacteriophageλ.By comparing the structures of the RecT and Red𝛽annealases,and the RecE and𝜆Exo exonucleases,we provide new insights into how the structures of these proteins dictate their *** the sequence conservation of the𝜆λExo and RecE exonucleases gives more profound insights into their critical functional ***,as recombineering accelerates and evolves in the laboratory,a better understanding of the mechanisms of the proteins behind this powerful technique will drive the development of improved and expanded capabilities in the future.

关键词： Recombineering Redβ λExo RecE RecT Protein structure Annealase Exonuclease

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Fitness and predation potential of Macrolophus pygmaeus reared under artificial conditions

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Insect Science 2011年第6期18卷 682-688页

作者： Bjorn Vandekerkhove Veronic De Puysseleyr Maarten Bonte Patrick De Clercq laboratory of Agrozoology Department of Crop Protection Faculty of Bioscience Engineering Ghent University Ghent Belgium

The biological parameters of Macrolophus pygmaeus Rambur afler prolonged rearing in the absence of plant materials were compared with those of conventionally plant-reared predators. When eggs of Ephestia kuehniella Zeller were provided as food, developmental and reproductive fitness of M. pygmaeus reared for over 30 consecutive generations using artificial living and oviposition substrates was similar to that of predators kept on tobacco leaves. Plantless-reared fifth instars of the predator also had similar predation rates on second instars of the tobacco aphid, Myzus persicae nicotianae Blackman, as their peers maintained on plant materials. In a further experiment, predation on aphid prey by fifth instar M. pygmaeus fed one of two egg yolk-based artificial diets was compared with that of nymphs fed E. kuehniella eggs. Despite their lower body weights, preda- tors produced on either artificial diet killed similar numbers of prey as their counterparts reared on lepidopteran eggs. Our study indicates that artificial rearing systems may be useful to further rationalize the production of this economically important biological control agent.

关键词： artificial diets artificial substrates biological control insect rearingMacrolophus pygmaeus quality assurance

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