检索结果-南通市图书馆

Vitamin A administration at birth promotes calf growth and intramuscular fat development in Angus beef cattle

Journal of Animal Science and Biotechnology 2018年第4期9卷 991-999页

作者： Corrine L.Harris Bo Wang Jeneane M.Deavila Jan R.Busboom Martin Maquivar Steven M.Parish Brent McCann Mark L.Nelson Min Du Department of Animal Sciences Washington State University state Key Laboratory of Animal Nutrition College of Animal Science and Technology China Agricultural University college of veterinary medicine Washington State University

Background: Marbling, or intramuscular fat, is an important factor contributing to the palatability of beef. Vitamin A, through its active metabolite, retinoic acid, promotes the formation of new fat cells(adipogenesis). As intramuscular adipogenesis is active during the neonatal stage, we hypothesized that vitamin A administration during the neonatal stage would enhance intramuscular adipogenesis and ***: Angus steer calves(n = 30), in a completely randomized design, were randomly allotted to three treatment groups at birth, receiving 0, 150,000, or 300,000 IU of vitamin A at both birth and one month of age.A biopsy of the biceps femoris muscle was collected at two months of age. After weaning at 210 d of age, steers were fed a backgrounding diet in a feedlot until 308 d of age, when they were transitioned to a high concentrate finishing diet and implanted with trenbolone/estradiol/tylosin mixture. Steers were harvested at an average of 438 d of age. All diets were formulated to meet nutrient ***: Weaning weight and weight during the backgrounding phase were linearly increased(P < 0.05) by vitamin A level, though no difference in body weight was observed at harvest. Intramuscular fat of steers at 308 d of age, measured by ultrasound, quadratically increased(P < 0.05) with vitamin A level from 4.0±0.26 % to 4.9±0.26 %.Similarly, carcass marbling score in the ribeye quadratically increased(P < 0.05).Conclusion: Administration of vitamin A at birth increased weaning weight and enhanced marbling fat ***, vitamin A administration provides a practical method for increasing marbling and early growth of beef cattle.

关键词： Beef Calf Cattle Marbling fat Quality Vitamin A

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Nicotine-induced upregulation of antioxidant protein Prx 1 in oral squamous cell carcinoma

引用

Chinese Science Bulletin 2013年第16期58卷 1912-1918页

作者： ZHAO YanHua ZHANG Min YAN Fei CASTO Bruce C TANG XiaoFei Division of Oral Pathology Beijing Institute of Dental Research School of Stomatology Capital Medical University Division of Medical Oncology Department of Internal Medicine Ohio State University College of Medicine and Comprehensive Cancer Center Division of Environmental Health Sciences Ohio State University College of Public Health

Nicotine is a source of exogenous oxidative stress, which is associated with the pathogenesis of numerous diseases including oral squamous cell carcinoma (OSCC), whereas an antioxidant protein, peroxiredoxin 1 (Prx 1), plays an important role in the modulation of this condition. This study was to investigate the association between Prx 1 and tobacco-induced oxidative stress. The expression of Prx 1 and GST in OSCC Tca8113 cells, which were pre-treated with nicotine, was determined. In the present study, MTT assay, reactive oxygen species (ROS) assay, RT-PCR and Western blot analyses, respectively, were conducted to assess cell viability, ROS level, and expression level of Prx 1 and GST in nicotine-treated Tca8113 cells. Nuclear factor kappa B (NF- B) expression was detected by immuno-fluorescence. Our results showed the growth of Tca8113 cells was increased in a dose-dependent manner when cells were treated with nicotine at concentrations from 0.1 to 10 mol/L, but the proliferation of the cells decreased at 100 mol/L. ROS levels increased in all groups treated with nicotine at concentrations of 0.1, 1, 10, or 100 mol/L for 24h. Prx 1 and GST mRNA and protein expression were up-regulated in cells treated with nicotine for the same time at different concentrations or at the same concentration for different times (P<0.05). NF-B was translocated from cytoplasm to nucleus, the expression of NF- B was increased in nucleus. These results suggest that up-regulation of Prx1 expression appears to be associated with tobacco-induced oxidative stress, which may play an important role in the pathogenesis of OSCC.

关键词：鳞状细胞癌蛋白表达抗氧化剂尼古丁口腔诱导氧化应激 RT-PCR

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of melatonin implantation on cashmere yield,fibre characteristics,duration of cashmere growth as well as growth and reproductive performance of Inner Mongolian cashmere goats

引用

Journal of Animal Science and Biotechnology 2015年第4期6卷 463-468页

作者： Chunhui Duan Jianhai Xu Changmian Sun Zhihai Jia Wei Zhang state Key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural University college of veterinary medicine China Agricultural University

Background： Exogenous melatonin could induce cashmere growth. However, induced growth of cashmere fleece by melatonin implants cannot be combined with the typical growth, resulting in earlier shedding followed by another cycle of cashmere growth. To address this issue, we examine the effects on the cashmere yield, fibre characteristics, and the growth and reproductive performance of cashmere goats of planned administration of melatonin. Methods： Eighteen half-sib, female goats were assigned to two treatments （n = 9） including a control and a treatment where melatonin （2 mg/kg BW） was implanted at the end of April and end of June. Cashmere growth and shedding were observed for approximately ] year following implantation. Fibre samples were collected monthly to determine cumulative cashmere length. Initiation and cessation dates for cashmere growth as well as the rate of cashmere growth were calculated. Cashmere yield, weight gain of dam, kidding date, litter size, and birth weight were also recorded. Results： Melatonin implantation increased cashmere yield by 34.5 % （control 553.7 g vs. melatonin 745.0 g; P〈 0.01）, cashmere length by 21.3 % （control 95.2 mm vs. melatonin 115.4 mm; P 〈 0.01）, and decreased fibre diameter by 4.4 % （control 14.6 pm vs. melatonin 14.0 IJm; P 〈 0.03）. In melatonin-treated goats, the average initiation date was earlier than in control goats （May 18, 2013 vs. July 2, 20]3; P〈 0.01） but there was a similar cessation date （March 22, 2014 vs. March 27, 2014）. Consequently, the duration of cashmere growth was longer in melatonin-treated goats than in control goats （307 vs.270 days; P 〈 0.01）. The final BW, average daily gain, kidding date, litter size, and birth weight were not influenced by melatonin implantation. Conclusions： These data indicate that melatonin implantation （2 mg/kg BW） on two occasions （late April and June） increased cashmere yield by combining the induced growth of cashmere fleece with the typical growth and decreased the fi

关键词： Cashmere goat Cashmere yield Fibre characteristics Growth performance Melatonin,Reproductive performance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Pseudorabies virus VHS protein abrogates interferon responses by blocking NF-κB and IRF3 nuclear translocation

引用

Virologica Sinica 2024年第4期39卷 587-599页

作者： Zhenfang Yan Jiayu Yue Yaxin Zhang Zhengyang Hou Dianyu Li Yanmei Yang Xiangrong Li Adi Idris Huixia Li Shasha Li Jingying Xie Ruofei Feng Key Laboratory of Biotechnology and Bioengineering of state Ethnic Biomedical Research Center Northwest Minzu UniversityLanzhou730030China college of Life Science and Engineering Northwest Minzu UniversityLanzhou730030China Gansu Tech Innovation Center of Animal Cell Biomedical Research CenterNorthwest Minzu UniversityLanzhou730030China Engineering Research Center of Key Technology and Industrialization of Cell-based Vaccine Ministry of EducationBiomedical Research CenterNorthwest Minzu UniversityLanzhou730030China Centre for Immunology and Infection Control School of Biomedical SciencesQueensland University of TechnologyKelvin GroveQueensland4702Australia

Herpesviruses antagonize host antiviral responses through a myriad of molecular strategies culminating in the death of the host *** virus(PRV)is a significant veterinary pathogen in pigs,causing neurological sequalae that ultimately lead to the animal's *** is known to trigger apoptotic cell death during the late stages of *** virion host shutdown protein(VHS)encoded by UL41 plays a crucial role in the PRV infection *** this study,we demonstrate that UL41 inhibits PRV-induced activation of inflammatory cytokine and negatively regulates the cGAS-STING-mediated antiviral activity by targeting IRF3,thereby inhibiting the translocation and phosphorylation of ***,mutating the conserved amino acid sites(E192,D194,and D195)in the RNase domain of UL41 or knocking down UL41 inhibits the immune evasion of PRV,suggesting that UL41 may play a crucial role in PRV's evasion of the host immune response during *** results enhance our understanding of how PRV structural proteins assist the virus in evading the host immune response.

关键词： Pseudorabies virus(PRV) UL41 IRF3 Interferon cGAS-STING

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neuropathophysiology of functional gastrointestinal disorders

引用

World Journal of Gastroenterology 2007年第9期13卷 1313-1332页

作者： Jackie D Wood Department of Physiology and Cell Biology and Internal Medicine The Ohio State University College of Medicine Columbus Ohio 43210-1218 United States

The investigative evidence and emerging concepts in neurogastroenterology implicate dysfunctions at the levels of the enteric and central nervous systems as underlying causes of the prominent symptoms of many of the functional gastrointestinal disorders. Neurogastroenterological research aims for improved understanding of the physiology and pathophysiology of the digestive subsystems from which the arrays of functional symptoms emerge. The key subsystems for defecation-related symptoms and visceral hyper- sensitivity are the intestinal secretory glands, the musculature and the nervous system that controls and integrates their activity. Abdominal pain and discomfort arising from these systems adds the dimension of sensory neurophysiology. This review details current concepts for the underlying pathophysiology in terms of the physiology of intestinal secretion, motility, nervous control, sensing function, immuno-neural communication and the brain-gut axis.

关键词： Neurogastroenterology Visceral pain Diarrhea Irritable bowel syndrome Constipation Stress Enteritis Enteric nervous system Neuroimmune communication Mast cells

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Identification of niclosamide as a novel antiviral agent against porcine epidemic diarrhea virus infection by targeting viral internalization

引用

Virologica Sinica 2023年第2期38卷 296-308页

作者： Yue Wang Huimin Huang Dongliang Li Chenxu Zhao Shuai Li Panpan Qin Yaqin Li Xia Yang Wenjuan Du Wentao Li Yongtao Li college of veterinary medicine Henan Agricultural UniversityZhengzhou450002China state Key Laboratory of Agricultural Microbiology College of Veterinary MedicineHuazhong Agricultural UniversityWuhan430070China Faculty of veterinary medicine Utrecht UniversityUtrecht3584 CLthe Netherlands Hubei Hongshan Laboratory Wuhan430070China

Porcine epidemic diarrhea virus(PEDV),an enteropathogenic coronavirus,has catastrophic impacts on the global pig ***,there remain no effective drugs against PEDV *** this study,we utilized a recombinant PEDV expressing renilla luciferase(PEDV-Rluc)to screen potential anti-PEDV agents from an FDAapproved drug library in Vero *** compounds were identified that significantly decreased luciferase activity of *** them,niclosamide was further characterized because it exhibited the most potent antiviral activity with the highest selectivity *** can efficiently inhibit viral RNA synthesis,protein expression and viral progeny production of classical and variant PEDV strains in a dose-dependent *** of addition assay showed that niclosamide exhibited potent anti-PEDV activity when added simultaneously with or after virus ***,niclosamide significantly inhibited the entry stage of PEDV infection by affecting viral internalization rather than viral attachment to *** addition,a combination with other small molecule inhibitors of endosomal acidification enhanced the anti-PEDV effect of niclosamide in *** together,these findings suggested that niclosamide is a novel antiviral agent that might provide a basis for the development of novel drug therapies against PEDV and other related pathogenic coronavirus infections.

关键词： Coronavirus Porcine epidemic diarrhea virus(PEDV) Niclosamide(NIC) Antiviral Virus entry Endocytosis Host-targeted antivirals

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

引用

Virologica Sinica 2023年第5期38卷 813-826页

作者： Yang Li Lele Xu Dian Jiao Zifang Zheng Zhihao Chen Yang Jing Zhiwei Li Zhiqian Maa Yingtong Feng Xuyang Guo Yumiao Wang Yuan He Haixue Zheng Shuqi Xiao state Key Laboratory for Animal Disease Control and Prevention College of Veterinary MedicineLanzhou UniversityLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou730046China college of veterinary medicine Northwest A&F UniversityYangling712100China

Porcine reproductive and respiratory syndrome(PRRS)is one of the most significant diseases affecting the pig industry *** PRRSV mutation rate is the highest among the RNA *** date,NADC30-like PRRSV and highly pathogenic PRRSV(HP-PRRSV)are the dominant epidemic strains in China;however,commercial vaccines do not always provide sufficient cross-protection,and the reasons for insufficient protection are *** study isolated a wild-type NADC30-like PRRSV,SX-YL1806,from Shaanxi *** challenge experiments in piglets showed that commercial modified live virus(MLV)vaccines provided good protection against ***,it could not provide sufficient protection against the novel strain *** explore the reasons for this phenomenon,we compared the genomic homology between the MLV strain and HP-PRRSV or NADC30-like PRRSV and found that the MLV strain had a lower genome similarity with NADC30-like *** neutralization assay showed that MLV-immune serum slightly promoted the homologous HP-PRRSV replication and significantly promoted the heterologous NADC30-like PRRSV strain replication in vitro,suggesting that antibody-dependent enhancement(ADE)might also play a role in decreasing MLV protective *** findings expand our understanding of the potential factors affecting the protective effect of PRRSV MLV vaccines against the NADC30-like strains.

关键词： NADC30-like PRRSV Modified live virus(MLV)vaccines Genomic similarity Antibody dependent enhancement(ADE) Cross protection

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Exogenous reference gene normalization for real-time reverse transcription-polymerase chain reaction analysis under dynamic endogenous transcription

引用

Neural Regeneration Research 2012年第14期7卷 1064-1072页

作者： Stephen Johnston Zachary Gallaher Krzysztof Czaja Programs in Neuroscience and Department of veterinary and Comparative Anatomy Pharmacology and Physiology College of Veterinary Medicine Washington State University Pullman WA 99164-6520 USA

Quantitative real-time reverse transcription-polymerase chain reaction （qPCR） is widely used to investigate transcriptional changes following experimental manipulations to the nervous system. Despite the widespread utilization of qPCR, the interpretation of results is marred by the lack of a suitable reference gene due to the dynamic nature of endogenous transcription. To address this inherent deficiency, we investigated the use of an exogenous spike-in mRNA, luciferase, as an internal reference gene for the 2ct normalization method. To induce dynamic transcription, we systemically administered capsaicin, a neurotoxJn selective for C-type sensory neurons expressing the TRPV-1 receptor, to adult male Sprague-Dawley rats. We later isolated nodose ganglia for qPCR analysis with the reference being either exogenous luciferase mRNA or the commonly used endogenous reference 13-111 tubulin. The exogenous luciferase mRNA reference clearly demonstrated the dynamic expression of the endogenous reference. Furthermore, variability of the endogenous reference would lead to misinterpretation of other genes of interest. In conclusion, traditional reference genes are often unstable under physiologically normal situations, and certainly unstable following the damage to the nervous system. The use of exogenous spike-in reference provides a consistent and easily implemented alternative for the analysis of qPCR data.

关键词： exogenous reference gene sensory ganglia reverse transcription-polymerase chain reaction normalization injury neural regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

通过机器学习开发基于作物基因型的抗病表型精准预测方法（英文）

引用

Engineering 2024年第9期 100-110页

作者：刘琪左世敏彭沙莎张昊彭烨李魏熊叶辉林润茂冯志明李慧慧杨俊王国梁康厚祥 state Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Zhongshan Biological Breeding Laboratory & Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding Agricultural College of Yangzhou University Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization and college of Agronomy Hunan Agricultural University state Key Laboratory of Crop Gene Resources and Breeding Institute of Crop Sciences Chinese Academy of Agricultural Sciences college of Plant Protection Hunan Agricultural University Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests Ministry of Education College of Plant Protection Hainan University MARA Key Laboratory of Surveillance and Management for Plant Quarantine Pests Department of Plant Biosecurity College of Plant Protection China Agricultural University Department of Plant Pathology Ohio State University

The traditional method of screening plants for disease resistance phenotype is both time-consuming and costly. Genomic selection offers a potential solution to improve efficiency, but accurately predicting plant disease resistance remains a challenge. In this study, we evaluated eight different machine learning(ML)methods, including random forest classification(RFC), support vector classifier(SVC), light gradient boosting machine(light GBM), random forest classification plus kinship(RFC＿K), support vector classification plus kinship(SVC＿K), light gradient boosting machine plus kinship(light GBM＿K), deep neural network genomic prediction(DNNGP), and densely connected convolutional networks(Dense Net), for predicting plant disease resistance. Our results demonstrate that the three plus kinship(K) methods developed in this study achieved high prediction accuracy. Specifically, these methods achieved accuracies of up to 95% for rice blast(RB), 85% for rice black-streaked dwarf virus(RBSDV), and 85% for rice sheath blight(RSB) when trained and applied to the rice diversity panel I(RDPI). Furthermore, the plus K models performed well in predicting wheat blast(WB) and wheat stripe rust(WSR) diseases, with mean accuracies of up to 90% and 93%, respectively. To assess the generalizability of our models, we applied the trained plus K methods to predict RB disease resistance in an independent population, rice diversity panel Ⅱ(RDPⅡ). Concurrently, we evaluated the RB resistance of RDPⅡ cultivars using spray inoculation. Comparing the predictions with the spray inoculation results, we found that the accuracy of the plus K methods reached 91%. These findings highlight the effectiveness of the plus K methods(RFC＿K, SVC＿K, and light GBM＿K) in accurately predicting plant disease resistance for RB, RBSDV, RSB,WB, and WSR. The methods developed in this study not only provide valuable strategies for predicting disease resistance, but also pave the way for using machine learning to str

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Enhancer variants reveal a conserved transcription factor network governed by PU.1 during osteoclast differentiation

引用

Bone Research 2018年第1期6卷 83-94页

作者： Heather A.Carey Blake E.Hildreth III Jennifer A.Geisler Mara C.Nickel Jennifer Cabrera Sankha Ghosh Yue Jiang Jing Yan James Lee Sandeep Makam Nicholas A.Young Giancarlo R.Valiente Wael N.Jarjour Kun Huang Thomas J.Rosol Ramiro E.Toribio Julia F.Charles Michael C.Ostrowski Sudarshana M.Sharma Department of Cancer Biology and Genetics and Comprehensive Cancer Center The Ohio State University Wexner Medical Center college of veterinary medicine The Ohio State University Department of Biochemistry and Molecular Biology and Hollings Cancer Center Medical University of South Carolina Division of Rheumatology Immunology and Allergy Department of Medicine Brigham and Women’s Hospital and Harvard Medical School Division of Rheumatology and Immunology Department of Internal Medicine The Ohio State University Wexner Medical Center Department of Biomedical Informatics The Ohio State University Wexner Medical Center

Genome-wide association studies(GWASs) have been instrumental in understanding complex phenotypic traits. However, they have rarely been used to understand lineage-specific pathways and functions that contribute to the trait. In this study, by integrating lineage-specific enhancers from mesenchymal and myeloid compartments with bone mineral density loci, we were able to segregate osteoblast-and osteoclast(OC)-specific functions. Specifically, in OCs, a PU.1-dependent transcription factor(TF)network was revealed. Deletion of PU.1 in OCs in mice resulted in severe osteopetrosis. Functional genomic analysis indicated PU.1 and MITF orchestrated a TF network essential for OC differentiation. Several of these TFs were regulated by cooperative binding of PU.1 with BRD4 to form superenhancers. Further, PU.1 is essential for conformational changes in the superenhancer region of Nfatc1. In summary, our study demonstrates that combining GWASs with genome-wide binding studies and model organisms could decipher lineage-specific pathways contributing to complex disease states.

关键词： Genome-wide GWASs MITF

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：