Identification of niclosamide as a novel antiviral agent against porcine epidemic diarrhea virus infection by targeting viral internalization
作者机构：College of Veterinary MedicineHenan Agricultural UniversityZhengzhou450002China State Key Laboratory of Agricultural MicrobiologyCollege of Veterinary MedicineHuazhong Agricultural UniversityWuhan430070China Faculty of Veterinary MedicineUtrecht UniversityUtrecht3584 CLthe Netherlands Hubei Hongshan LaboratoryWuhan430070China
出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)
年 卷 期：2023年第38卷第2期
页 面：296-308页
核心收录：
学科分类：090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学]
基 金：supported by the National Natural Science Foundation of China(Grant No.31602033 and 32172839) the China Scholarship Council under grant 201908410129
主 题：Coronavirus Porcine epidemic diarrhea virus(PEDV) Niclosamide(NIC) Antiviral Virus entry Endocytosis Host-targeted antivirals
摘 要：Porcine epidemic diarrhea virus(PEDV),an enteropathogenic coronavirus,has catastrophic impacts on the global pig ***,there remain no effective drugs against PEDV *** this study,we utilized a recombinant PEDV expressing renilla luciferase(PEDV-Rluc)to screen potential anti-PEDV agents from an FDAapproved drug library in Vero *** compounds were identified that significantly decreased luciferase activity of *** them,niclosamide was further characterized because it exhibited the most potent antiviral activity with the highest selectivity *** can efficiently inhibit viral RNA synthesis,protein expression and viral progeny production of classical and variant PEDV strains in a dose-dependent *** of addition assay showed that niclosamide exhibited potent anti-PEDV activity when added simultaneously with or after virus ***,niclosamide significantly inhibited the entry stage of PEDV infection by affecting viral internalization rather than viral attachment to *** addition,a combination with other small molecule inhibitors of endosomal acidification enhanced the anti-PEDV effect of niclosamide in *** together,these findings suggested that niclosamide is a novel antiviral agent that might provide a basis for the development of novel drug therapies against PEDV and other related pathogenic coronavirus infections.
维普期刊数据库