Nicotine-induced upregulation of antioxidant protein Prx 1 in oral squamous cell carcinoma

作     者：ZHAO YanHua ZHANG Min YAN Fei CASTO Bruce C TANG XiaoFei 

作者机构：Division of Oral Pathology Beijing Institute of Dental Research School of Stomatology Capital Medical University Division of Medical Oncology Department of Internal Medicine Ohio State University College of Medicine and Comprehensive Cancer Center Division of Environmental Health Sciences Ohio State University College of Public Health 

出 版 物：《Chinese Science Bulletin》 (Chinese Science Bulletin)

年 卷 期：2013年第58卷第16期

页      面：1912-1918页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the Beijing Natural Science Foundation(7102065) the National Natural Science Foundation of China(81070836) 

主　　题：鳞状细胞癌 蛋白表达 抗氧化剂 尼古丁 口腔 诱导 氧化应激 RT-PCR 

摘      要：Nicotine is a source of exogenous oxidative stress, which is associated with the pathogenesis of numerous diseases including oral squamous cell carcinoma (OSCC), whereas an antioxidant protein, peroxiredoxin 1 (Prx 1), plays an important role in the modulation of this condition. This study was to investigate the association between Prx 1 and tobacco-induced oxidative stress. The expression of Prx 1 and GST in OSCC Tca8113 cells, which were pre-treated with nicotine, was determined. In the present study, MTT assay, reactive oxygen species (ROS) assay, RT-PCR and Western blot analyses, respectively, were conducted to assess cell viability, ROS level, and expression level of Prx 1 and GST in nicotine-treated Tca8113 cells. Nuclear factor kappa B (NF- B) expression was detected by immuno-fluorescence. Our results showed the growth of Tca8113 cells was increased in a dose-dependent manner when cells were treated with nicotine at concentrations from 0.1 to 10 mol/L, but the proliferation of the cells decreased at 100 mol/L. ROS levels increased in all groups treated with nicotine at concentrations of 0.1, 1, 10, or 100 mol/L for 24h. Prx 1 and GST mRNA and protein expression were up-regulated in cells treated with nicotine for the same time at different concentrations or at the same concentration for different times (P0.05). NF-B was translocated from cytoplasm to nucleus, the expression of NF- B was increased in nucleus. These results suggest that up-regulation of Prx1 expression appears to be associated with tobacco-induced oxidative stress, which may play an important role in the pathogenesis of OSCC.