Genomic similarity and antibody-dependent enhancement of immune serum potentially affect the protective efficacy of commercial MLV vaccines against NADC30-like PRRSV

作     者：Yang Li Lele Xu Dian Jiao Zifang Zheng Zhihao Chen Yang Jing Zhiwei Li Zhiqian Maa Yingtong Feng Xuyang Guo Yumiao Wang Yuan He Haixue Zheng Shuqi Xiao Yang Li;Lele Xu;Dian Jiao;Zifang Zheng;Zhihao Chen;Yang Jing;Zhiwei Li;Zhiqian Ma;Yingtong Feng;Xuyang Guo;Yumiao Wang;Yuan He;Haixue Zheng;Shuqi Xiao

作者机构：State Key Laboratory for Animal Disease Control and PreventionCollege of Veterinary MedicineLanzhou UniversityLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou730046China College of Veterinary MedicineNorthwest A&F UniversityYangling712100China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2023年第38卷第5期

页      面：813-826页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学] 

基　　金：This research was supported by the National Natural Science Foundation of China(32172846) the Earmarked Fund for CARS-35,the Science and Technology Major Project of Gansu Province(22ZD6NA001) the Science Foundation for Distinguished Young Scholars of Shaanxi Province(2021JC-18) the Natural Science Foundation of Gansu Province(23JRRA1153) the Science and Technology Plan Project of Gansu Province(23JRRA561) the Chinese Academy of Agricultural Science and Technology Innovation Project(CAAS-ASTIP-JBGS-20210602) the Strategic Priority Research Program of the National Center of Technology Innovation for Pigs(NCTIP-XD/C03) 

主　　题：NADC30-like PRRSV Modified live virus(MLV)vaccines Genomic similarity Antibody dependent enhancement(ADE) Cross protection 

摘      要：Porcine reproductive and respiratory syndrome(PRRS)is one of the most significant diseases affecting the pig industry *** PRRSV mutation rate is the highest among the RNA *** date,NADC30-like PRRSV and highly pathogenic PRRSV(HP-PRRSV)are the dominant epidemic strains in China;however,commercial vaccines do not always provide sufficient cross-protection,and the reasons for insufficient protection are *** study isolated a wild-type NADC30-like PRRSV,SX-YL1806,from Shaanxi *** challenge experiments in piglets showed that commercial modified live virus(MLV)vaccines provided good protection against ***,it could not provide sufficient protection against the novel strain *** explore the reasons for this phenomenon,we compared the genomic homology between the MLV strain and HP-PRRSV or NADC30-like PRRSV and found that the MLV strain had a lower genome similarity with NADC30-like *** neutralization assay showed that MLV-immune serum slightly promoted the homologous HP-PRRSV replication and significantly promoted the heterologous NADC30-like PRRSV strain replication in vitro,suggesting that antibody-dependent enhancement(ADE)might also play a role in decreasing MLV protective *** findings expand our understanding of the potential factors affecting the protective effect of PRRSV MLV vaccines against the NADC30-like strains.