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A 2-kb Mycovirus Converts a Pathogenic Fungus into a Beneficial Endophyte for Brassica Protection and Yield Enhancement

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Molecular plant 2020年第10期13卷 1420-1433页

作者： Hongxiang Zhang Jiatao Xie Yanping Fu Jiasen Cheng Zheng Qu Zhenzhen Zhao Shufen Cheng Tao Chen Bo Li Qianqian Wang Xinqiang Liu Binnian Tian David B.Collinge Daohong Jiang State key laboratory of Agricultural Microbiology College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanHubei Province 430070China The Provincial key lab of plant pathology of hubei province Huazhong Agricultural UniversityWuhanChina Department of plant and Environmental Sciences University of Copenhagen1871 Frederiksberg CDenmark

Mycoviruses are viruses that infect fungi,and hypovirulence-associated mycoviruses have the potential to control fungal ***,it is unclear how mycovirus-mediated hypovirulent strains live and survive in the field,and no mycovirus has been applied for field crop *** this study,we found that a previously identified small DNA mycovirus(SsHADV-1)can convert its host,Sclerotinia sclerotiorum,from a typical necrotrophic pathogen to a beneficial endophytic ***-1 downregulates the expression of key pathogenicity factor genes in *** during *** growing in rapeseed,the SsHADV-1-infected strain DT-8 significantly regulates the expression of rapeseed genes involved in defense,hormone signaling,and circadian rhythm *** a result,plant growth is promoted and disease resistance is *** experiments showed that spraying DT-8 at the early flowering stage can reduce the disease severity of rapeseed stem rot by 67.6%and improve yield by 14.9%.Moreover,we discovered that SsHADV-1 could also infect other *** strains on DT-8-inoculated plants and that DT-8 could be recovered from dead *** findings suggest that the mycoviruses may have the ability to shape the origin of *** discoveries suggest that mycoviruses may influence the origin of endophytism and may also offer a novel strategy for disease control in which mycovirus-infected strains are used to improve crop health and release mycoviruses into the field.

关键词： mycovirus Sclerotinia sclerotiorum endophyte rapeseed stem rot biological control Brassica napus

MoLrp1-mediated signaling induces nuclear accumulation of MoMsn2 to facilitate fatty acid oxidation for infectious growth of the rice blast fungus

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plant Communications 2023年第4期4卷 160-173页

作者： Ting Zhang Xingyu Wang Xue Li Ya-Nan Li Yuhe Li Shuang Wu Lele Xu Ruiwen Zhou Jing Yang Guotian Li Xinyu Liu Xiaobo Zheng Zhengguang Zhang Haifeng Zhang Department of plant pathology College of Plant ProtectionNanjing Agricultural Universityand Key Laboratory of Integrated Management of Crop Diseases and PestsMinistry of EducationNanjingChina The key laboratory of plant Immunity Nanjing Agricultural UniversityNanjingChina State key laboratory of Agricultural Microbiology Hubei Hongshan Laboratorythe Provincial Key Laboratory of Plant Pathology of Hubei ProvinceCollege of Plant Science&TechnologyHuazhong Agricultural UniversityWuhanChina

Fatty acid b-oxidation is critical for fatty acid degradation and cellular *** the rice blast fungus Magnaporthe oryzae,fatty acid b-oxidation is reported to be important mainly for turgor generation in the ***,the role of fatty acid b-oxidation during invasive hyphal growth is rarely *** demonstrated that blocking peroxisomal fatty acid b-oxidation impaired lipid droplet(LD)degradation and infectious growth of *** found that the key regulator of pathogenesis,MoMsn2,which we identified previously,is involved in fatty acid b-oxidation by targeting MoDCI1(encoding dienoyl-coenzyme A[CoA]isomerase),which is also important for LD degradation and infectious *** observations revealed that MoMsn2 accumulated from the cytosol to the nucleus during early infection or upon treatment with *** determined that the low-density lipoprotein receptor-related protein MoLrp1,which is also involved in fatty acid b-oxidation and infectious growth,plays a critical role in the accumulation of MoMsn2 from the cytosol to the nucleus by activating the cyclic AMP signaling *** results provide new insights into the importance of fatty acid oxidation during invasive hyphal growth,which is modulated by MoMsn2 and its related signaling pathways in ***.

关键词： transcription factor MoMsn2 target gene fatty acid b-oxidation cAMP signaling infectious growth

bZIP Transcription Factor UvATF21 Mediates Vegetative Growth,Conidiation,Stress Tolerance and Is Required for Full Virulence of Rice False Smut Fungus Ustilaginoidea virens

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Rice science 2023年第1期30卷 50-57页

作者： LIU Yueran QU Jinsong WANG Yufu YIN Weixiao LUO Chaoxi hubei key laboratory of plant pathology/College of plant Science and Technology Huazhong Agricultural UniversityWuhan 430070China

bZIP proteins are widely distributed in eukaryotic organisms and regulate a diverse range of physiological *** bZIP proteins have previously been identified in Ustilaginoidea ***,the biological roles of these bZIP proteins in this pathogen are still ***,one of these bZIP protein coding genes,UvATF21,was functionally *** deletion of UvATF21resulted in reduced conidiation and pathogenicity despite of the increased vegetative *** deletion mutants also significantly decreased the sensitivity to osmotic and oxidative ***,deletion of UvATF21 exhibited different performances to cell wall integrity *** results indicated that UvATF21 played crucial roles in vegetative growth,conidiation,stress response,and full virulence in ***.

关键词： Ustilaginoidea virens bZIP protein conidiation stress response pathogenicity

Enrichment of bacteria involved in the nitrogen cycle and plant growth promotion in soil by sclerotia of rice sheath blight fungus

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Stress Biology 2022年第1期2卷 299-311页

作者： Mirza Abid Mehmood Yanping Fu Huizhang Zhao Jiasen Cheng Jiatao Xie Daohong Jiang State key laboratory of Agricultural Microbiology Huazhong Agricultural UniversityHubei ProvinceWuhan 430070China plant pathology Institute of Plant ProtectionMNS University of AgricultureMultan 60000Pakistan Provincial key laboratory of plant pathology of hubei province College of Plant Science and TechnologyHuazhong Agricultural UniversityHubei ProvinceWuhan 430070China hubei Hongshan laboratory Wuhan 430070China

Rice sheath blight pathogen,Rhizoctonia solani,produces numerous sclerotia to *** a rich source of nutrients in the soil,sclerotia may lead to the change of soil *** this purpose,we amended the sclerotia of *** in soil and analyzed the changes in bacterial microbiota within the soil at different time *** the phyla level,Proteobacteria,Acidobacteria,Bacteroidetes,Actinobacteria,Chloroflexi and Firmicutes showed varied abundance in the amended soil samples compared to those in the *** increased abundance of ammonia-oxidizing bacterium(AOB)Nitrosospira and Nitrite oxidizing bacteria(NOB)i.e.,Nitrospira was observed,where the latter is reportedly involved in the nitrifier ***,Thiobacillus,Gemmatimonas,Anaeromyxobacter and Geobacter,the vital players in denitrification,N2O reduction and reductive nitrogen transformation,respectively,depicted enhanced abundance in *** sclerotia-amended ***,asymbiotic nitrogen-fixing bacteria,notably,Azotobacter as well as Microvirga and Phenylobacterium with nitrogen-fixing potential also enriched in the amended samples compared to the *** growth promoting bacteria,such as Kribbella,Chitinophaga and Flavisolibacter also enriched in the sclerotia-amended *** per our knowledge,this study is of its kind where pathogenic fungal sclerotia activated microbes with a potential role in N transformation and provided clues about the ecological functions of *** sclerotia on the stimulation of bacterial genera involved in different processes of N-cycle within the soil in the absence of host plants.

关键词： Rice sheath blight Rhizoctonia solani Sclerotia Reductive nitrogen transformation Nitrogen fixation Soil microbiome

Identification of the nitrogen-fixing Pseudomonas stutzeri major flagellar gene regulator FleQ and its role in biofilm formation and root colonization

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Journal of Integrative Agriculture 2016年第2期15卷 339-348页

作者： MA Yao ZHANG Qiu-lei YANG Zhi-min LI Yun YAN Yong-liang PING Shu-zhen ZHANG Li-wen LIN Min LU Wei College of Biological Sciences China Agricultural University Biotechnology Research Institute Chinese Academy of Agricultural Sciences The key laboratory of plant pathology of hubei province/College of plant Science and Technology Huazhong Agricultural University

Flagellar biosynthesis and motility are subject to a four-tiered transcriptional regulatory circuit in Pseudomonas,and the master regulator FleQ appears to be the highest-level regulator in this hierarchical regulatory *** stutzeri A1501 is motile by a polar flagellum;however,the motility and regulatory mechanisms involved in this process are ***,we searched the A1501 genome for flagella and motility genes and found that approximately 50 genes,which were distributed in three non-contiguous chromosomal regions,contribute to the formation,regulation and function of the *** non-polar mutation of fleQ impaired flagellar biosynthesis,motility and root colonization but enhanced biofilm *** positively regulates the expression of flagellar class Ⅱ-Ⅳ genes,suggesting a regulatory cascade that is coordinated similar to that of the well-known *** on our results,we propose that flagellar genes in *** A1501 are regulated in a cascade regulated by FleQ and that flagellum-driven motility properties may be necessary for competitive rhizosphere colonization.

关键词： FleQ Pseudomonas stutzeri flagellar biosynthesis biofilm formation root colonization

Silicon Pick-up of Interstitial-Free Steel From MgO-SiC Refractories

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Journal of Iron and Steel Research International 2008年第3期15卷 92-94,69页

作者： WEI Yao-wu LI Nan CHEN Xiao-xia HU Tie-shan RAO Jiang-ping TAO Xiao-lin hubei province key lab of Refarctories and Ceramics Wuhan University of Science and Technology Wuhan 430081 Hubei China No. 3 Steelmaking plant of Wuhan Iron and Steel Group Corporation Wuhan 430082 Hubei China

The silicon pick-up of interstitial-free steel （IF steel） from resin bonded MgO-SiC refractories is studied via a medium frequency induction furnace. Samples of IF steel were heated in MgO-SiC crucibles with mould flux cover at 1 600 ℃ for 180 min. The silicon contents of IF steel samples taken from the molten steel in every 30 min were examined and the refractories after heating were investigated by electron probe microanalysis （EPMA） with energy dispersive spectrometer （EDS）. The silicon content of steel rapidly increased in the first 90 min of beating, and then decreased because of the formation of liquid layer on the refractory. Liquid layer separated the liquid steel from the bulk refractory and stopped the pick-up of silicon. At the same time, desilication via oxygen through the liquid layer and flux started to reduce the silicon content of the liquid steel

关键词： MgO-SiC refractory silicon carbide silicon content steel

Suppression of ETI by PTI priming to balance plant growth and defense through an MPK3/MPK6-WRKYs-PP2Cs module

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Molecular plant 2023年第5期16卷 903-918页

作者： Dacheng Wang Lirong Wei Ting Liu Jinbiao Ma keyi Huang Huimin Guo Yufen Huang Lei Zhang Jing Zhao Kenichi Tsuda Yiming Wang key laboratory of Biological Interactions and Crop Health Department of Plant PathologyNanjing Agricultural UniversityNanjing 210095China key laboratory of Integrated Management of Crop Disease and Pests Ministry of EducationNanjing Agricultural UniversityNanjingChina State key laboratory of Agricultural Microbiology Hubei Hongshan LaboratoryHubei Key Lab of Plant PathologyCollege of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina

Pattern-triggered immunity(PTI)and effector-triggered immunity(ETI)are required for host defense against *** PTI and ETI are intimately connected,the underlying molecular mechanisms remain *** this study,we demonstrate that flg22 priming attenuates Pseudomonas syringae *** DC3000(Pst)AvrRpt2-induced hypersensitive cell death,resistance,and biomass reduction in ***-activated protein kinases(MAPKs)are key signaling regulators of PTI and *** absence of MPK3 and MPK6 significantly reduces pre-PTI-mediated ETI suppression(PES).We found that MPK3/MPK6 interact with and phosphorylate the downstream transcription factor WRKY18,which regulates the expression of AP2C1 and PP2C5,two genes encoding protein ***,we observed that the PTI-suppressed ETI-triggered cell death,MAPK activation,and growth retardation are significantly attenuated in wrky18/40/60 and ap2c1 pp2c5 *** together,our results suggest that the MPK3/MPK6-WRKYs-PP2Cs module underlies PES and is essential for the maintenance of plant fitness during ETI.

关键词： PAMP-triggered immunity effector-triggered immunity MAPK,WRKY18 protein phosphatase

Melanin,DNA replication,and autophagy affect appressorium development in Setosphaeria turcica by regulating glycerol accumulation and metabolism

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Journal of Integrative Agriculture 2022年第3期21卷 762-773页

作者： GUO Xiao-yue LIU Ning LIU Bing-hui ZHOU Li-hong CAO Zhi-yan HAN Jian-min DONG Jin-gao State key laboratory of North China Crop Improvement and Regulation/key laboratory of Hebei province for plant Physiology and Molecular pathology/Mycotoxin and Molecular plant pathology laboratory Hebei Agricultural UniversityBaoding 071001P.R.China

Setosphaeria turcica(*** turcicum)is the pathogenic fungus of maize(Zea mays)that causes northern leaf blight,which is a major maize disease *** appressoria are highly specialized infection structures formed by germinated conidia of *** that infect maize *** appressorium penetrates the plant cuticle by generating turgor,and glycerol is known to be the main source of the ***,the infection position penetrated by the appressorium on maize leaves was investigated,most of the germinated conidia entered the leaf interior by directly penetrating the epidermal cells,and the appressorium structure was necessary for the infection,whether it occurred through epidermal cells or ***,to investigate the effects of key factors in the development of the appressorium,we studied the effects of three inhibitors,including a melanin inhibitor(tricyclazole,TCZ),a DNA replication inhibitor(hydroxyurea,HU),and an autophagy inhibitor(3-methyladenine,3-MA),on appressorium turgor and glycerol *** results,appressorium turgor pressure and glycerol concentration in the appressorium reached their highest levels at the mature stage of the appressorium under the control and inhibitor *** three inhibitors had the greatest effects on appressorium turgor pressure at this *** and liposomes are the main substances producing *** was also found inhibitors affected the distribution of glycogen and liposomes,which were detected in the conidia,the germ tube,and the appressorium during appressorium *** study provides profound insight into the relationship between appressorium turgor pressure and glycerol content,which was affected by the synthesis of melanin,DNA replication,and autophagy in the developing appressorium during a *** infection.

关键词： melanin DNA replication autophagy appressorium development Setosphaeria turcica glycerol

Identification and virulence test of a new pathogen that causes verticillium striping on rapeseed in northwestern China

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Oil Crop Science 2024年第1期9卷 8-19页

作者： We Si Ruisheng Wang Mingde Wu Long Yang Guoqing Li Jing Zhang State key laboratory of Agricultural Microbiology and key laboratory of plant pathology of hubei province Huazhong Agricultural UniversityWuhan 430070China Qinghai Academy of Agriculture and Forestry Sciences Qinghai UniversityXiningQinghai Province810016China

Five stems of rapeseed with abundant black microsclerotia were collected from Huangyuan County of Qinghai province,China,and fungal isolates were obtained from the *** were identified based on morphology,molecular features and specific PCR *** results showed that the 10 fungal isolates belonged to Verticillium longisporum lineage A1/*** of the 10 isolates(HW7-1)was tested for virulence on three species of rapeseed,including *** Zhongshuang 9,*** Qingyou 9 and *** Tayou 2 by conidia inoculation of HW7-1 on roots of young *** seedlings were inoculated with *** conidia or water *** seedlings of these treatments were transplanted in culture mix and incubated in a growth chamber(20℃).Results suggested that the control seedlings of three cultivars appeared quite healthy,while the seedlings inoculated with HW7-1 turned yellowing leaves,seedling stunting or even death after 22 days *** was re-isolated from he yellow leaves,thus fulfilling Koch's ***,compared to the control treatments,inoculation with HW7-1 caused flowering delay and seed yield reduction on Tayou 2 with production of microsclerotia on the *** our knowledge,this is the first report of *** lineage A1/D3 on rapeseed in northwestern China.

关键词： Verticillium longisporum lineage A1/D3 Virulence Rapeseed China