Active DNA demethylation regulates MAMP-triggered immune priming in Arabidopsis

作     者：Mengling Huang Ying Zhang Ying Wang Jiatao Xie Jiasen Cheng Yanping Fu Daohong Jiang Xiao Yu Bo Li Mengling Huang;Ying Zhang;Ying Wang;Jiatao Xie;Jiasen Cheng;Yanping Fu;Daohong Jiang;Xiao Yu;Bo Li

作者机构：State Key Laboratory of Agricultural MicrobiologyHuazhong Agricultural UniversityWuhanHubei 430070China The Provincial Key Lab of Plant Pathology of Hubei ProvinceCollege of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanHubei 430070China Hubei Hongshan LaboratoryWuhanHubei 430070China 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2022年第49卷第8期

页      面：796-809页

核心收录：

学科分类：09[农学] 0903[农学-农业资源与环境] 

基　　金：supported by the National Natural Science Foundation of China (31770278, 31970125 to B.L.) Fundamental Research Funds for the Central Universities, Huazhong Agricultural University Scientific & Technological Self-innovation Foundation (2021ZKPY011, 2017RC001 to B.L.) 

主　　题：Pattern-triggered immunity Immune priming Defense gene expression DNA demethylation Immune signal transduction 

摘      要：Plants recognize microbe-associated molecular patterns(MAMPs)to activate immune responses and defense priming to defend against pathogen *** regulation of gene expression is crucial for plant immunity and is mediated by multiple factors,including DNA ***,it remains unknown whether and how DNA demethylation contributes to immune responses in MAMPtriggered ***,we report that active DNA demethylation is required for MAMP-triggered immunity to bacterial *** rdd-2 triple mutant carrying mutations in ROS1,DML2,and DML3 that encode DNA glycosylases,which are key DNA demethylation enzymes,exhibits compromised immune responses triggered by the MAMPs fig22 and ***-wide methylome analysis reveals that fig22 induces rapid and specific DNA demethylation in an RDD-dependent *** expression levels of salicylic acid signaling-related and phytoalexin biosynthesis-related genes are tightly associated with the fig22-induced promoter *** compromised accumulation of priming compounds and antimicrobial metabolites ultimately leads to a defense priming defect in the rdd-2 *** results reveal the critical role of active DNA demethylation in the MAMP-triggered immune response and provide unique insight into the molecular mechanism of fig22-modulated DNA demethylation.