Identification of the nitrogen-fixing Pseudomonas stutzeri major flagellar gene regulator FleQ and its role in biofilm formation and root colonization

作     者：MA Yao ZHANG Qiu-lei YANG Zhi-min LI Yun YAN Yong-liang PING Shu-zhen ZHANG Li-wen LIN Min LU Wei 

作者机构：College of Biological SciencesChina Agricultural University Biotechnology Research InstituteChinese Academy of Agricultural Sciences The Key Laboratory of Plant Pathology of Hubei Province/College of Plant Science and TechnologyHuazhong Agricultural University 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2016年第15卷第2期

页      面：339-348页

核心收录：

学科分类：09[农学] 0903[农学-农业资源与环境] 

基　　金：supported by grants from the National Basic Research(973) Program of China(2015CB755700) the National High-Tech R&D(863) Program of China (2012AA02A703) the National Natural Science Foundation of China(31170081) the Special Fund for Agro-scientific Research in the Public Interest,China(201103007) the Guangdong Innovative and Entrepreneurial Research Team Program,China(2013S033) 

主　　题：FleQ Pseudomonas stutzeri flagellar biosynthesis biofilm formation root colonization 

摘      要：Flagellar biosynthesis and motility are subject to a four-tiered transcriptional regulatory circuit in Pseudomonas,and the master regulator FleQ appears to be the highest-level regulator in this hierarchical regulatory *** stutzeri A1501 is motile by a polar flagellum;however,the motility and regulatory mechanisms involved in this process are ***,we searched the A1501 genome for flagella and motility genes and found that approximately 50 genes,which were distributed in three non-contiguous chromosomal regions,contribute to the formation,regulation and function of the *** non-polar mutation of fleQ impaired flagellar biosynthesis,motility and root colonization but enhanced biofilm *** positively regulates the expression of flagellar class Ⅱ-Ⅳ genes,suggesting a regulatory cascade that is coordinated similar to that of the well-known *** on our results,we propose that flagellar genes in *** A1501 are regulated in a cascade regulated by FleQ and that flagellum-driven motility properties may be necessary for competitive rhizosphere colonization.