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Stocking density effects on growth and stress response of juvenile turbot(Sco.hthalmus maximus) reared in land-based recirculating aquaculture system

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Acta Oceanologica Sinica 2017年第10期36卷 31-38页

作者： LIU Baoliang JIA Rui ZHAO Kuifeng WANG Guowen LEI Jilin HUANG Bin Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Key Laboratory of Sustainable Development of Marine Fisheries Ministry of Agriculture Qingdao Key Laboratory for Marine Fish Breeding and biotechnology Wuxi Fisheries co.lege Nanjing Agricultural University shandong Oriental Ocean Sci-Tech co. Ltd

Stocking density is widely reco.nized as a critical factor in aquaculture and a potential source of long-term *** influence of stocking density on growth and stress response of juvenile turbot（Sco.hthalmus maximus, ~3–75g, initial to final weight） was examined in fish held under low（LD, ~0.21–5.31 kg/m^2, initial to final density）,medium（MD, ~0.42–10.81 kg/m^2） and high stocking density（HD, ~0.63–14.27 kg/m^2） for 120 days in a recirculating aquaculture system（RAS）. In this trial, the growth curve for weight of juvenile turbot in RAS, all fitted by the Schnute model. No significant difference was found in growth performance among the three densities until at the final sampling（Day 120）. The final weight and body weight increase（BWI） in the HD group were significantly lower than in other groups（P〈0.05, weight：（75.83±2.49） g,（75.39±2.08） g,（65.72±2.86） g and BWI：（2 436.12±28.10）%,（2 421.29±4.64）%,（2 097.88±20.99）% in LD, MD and HD groups, respectively）. Similarly, the specific growth rate（SGR）, feed co.version ratio（FCR） and co.fficient of variation for weight（CV_w） were adversely affected by high stocking density（P〈0.05）. However, there was no difference in survival and Fulton＇s co.dition factor（K） of turbot among the different groups. Physiological analyses demonstrated a clear increase in the plasma co.tisol level and an obvious decrease in growth hormone（GH） co.centration in the HD group on Day120（P〈0.05）. There was no significant effect of stocking density on plasma gluco.e, Cl– and protein levels. All these findings would provide a reference for selecting the optimal stocking density of juvenile turbot in RAS.

关键词： growth performance recirculating aquaculture system Sco.hthalmus maximus stress physiology stocking density

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Cut-dip-budding delivery system enables genetic modifications in plants without tissue culture

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The Innovation 2023年第1期4卷 1-8页

作者： Xuesong Cao Hongtao Xie Minglei Song Jinghua Lu Ping Ma Boyu Huang Mugui Wang Yifu Tian Fan Chen Jun Peng Zhaobo Lang Guofu Li Jian-Kang Zhu Shanghai Center for Plant Stress Biology Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 201602China shandong shunfeng biotechnology co.Ltd. Jinan 250000China University of the Chinese Academy of Sciences Beijing 100049China Institute of Advanced biotechnology and School of Life Sciences Southern University of Science and TechnologyShenzhen 518055China Center for Advanced Bioindustry Technologies Chinese Academy of Agricultural SciencesBeijing 100081China Hainan Yazhou Bay Seed Laboratory Sanya 572025China

Of the more than 370000 species of higher plants in nature,fewer than 0.1%can be geneticallymodified due to limitations of the current gene delivery *** for those that can be genetically modified,the modification involves a tedious and co.tly tissue culture ***,we describe an extremely simple cut-dip-budding(CDB)delivery system,which uses Agrobacterium rhizogene to inoculate explants,generating transformed roots that produce transformed buds due to root *** have successfully used CDB to achieve the heritable transformation of plant species inmultiple plant families,including two herbaceous plants(Taraxacum kok-saghyz and co.onilla varia),a tuberous root plant(sweet potato),and three woody plant species(Ailanthus altissima,Aralia elata,and Clerodendrum chinense).These plants have previously been difficult or impossible to transform,but the CDB method enabled efficient transformation or gene editing in them using a very simple explant dipping protoco.,under non-sterile co.ditions and without the need for tissue *** work suggests that large numbers of plants co.ld be amenable to genetic modifications using the CDB method.

关键词： process. enable transformed

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Cas12a-based on-site, rapid detection of genetically modified crops

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Journal of Integrative Plant Biology 2022年第10期64卷 1856-1859页

作者： Zhiqiang Duan Xiaoliang Yang Xingkun Ji Ying Chen Xiaomu Niu Anping Guo Jian‐Kang Zhu Feng Li Zhaobo Lang Hui Zhao Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off‐Season Reproduction Regions Institute of Tropical Bioscience and Biotechnology&San Ya Research InstituteChinese Academy of Tropical Agricultural SciencesHaikou 571101China Bellagen biotechnology co.Ltd. Jinan 250000China co.lege of Life Sciences Shandong Normal UniversityJinan 250014China Institute of Advanced biotechnology and School of Life Sciences Southern University of Science and TechnologyShenzhen 518055China Center for Advanced Bioindustry Technologies Chinese Academy of Agricultural SciencesBeijing 100081China Hainan Yazhou Bay Seed Lab SanyaHainan 572024China Shanghai Center for Plant Stress Biology CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 201602China

Genetically modified(GM)crops that impart herbicide resistance,improved tolerance to biotic and abiotic stresses,and enhanced yield or nutritional quality have been developed and grown worldwide(ISAAA Brief 55-2019:International Servicefor theAcquisition ofAgri-biotech Applications(ISAAA),2019).In response topublic co.cern on genetically modified organisms(GMOs),DNA-based and protein-based methods have been developed to detect GMOs infood/feed samples(Fraitureetal.,2015).These methods,however,require expensive equipment and technical expertise and are unsuitable for field diagnosis(DNA-based methods)or cannot detect non-co.ing sequences such as promoters or terminators(protein-based methods).

关键词： crops modified diagnosis

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Eco.omic value and exploiting approaches of sea asparagus, a seawater-irrigated vegetable

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Agricultural Sciences 2013年第9期4卷 40-44页

作者： Litian Feng Bin Ji Bin Su Department of Eco.ogical Science Faculty of Earth and Life Sciences VU University Amsterdam The Netherlands Jinan Winsir biotechnology co. Ltd. Jinan China shandong Seawater Vegetable biotechnology Development co. Ltd. Jinan shandong Seawater Vegetable biotechnology Development co. Ltd.

Sea asparagus is a newly emerging seawater-irrigated vegetable. Firstly, the eco.omic value of sea asparagus was introduced, and then, someexploiting approaches of sea asparagus werefully reviewed. The exploiting approaches mainly include fresh vegetable, pickle, bio-salt, health-care products, animal feed, vegetable oil, bio-diesel and skin-care products.

关键词： Sea Asparagus Seawater-Irrigated Vegetable Eco.omic Value Exploiting Approaches Bio-Salt

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Development of environmentally friendly biological algicide and biochemical analysis of inhibitory effect of diatom Skeletonema co.tatum

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Chinese Chemical Letters 2022年第3期33卷 1358-1364页

作者： Jie Yang Qingzheng Zhu Jinlong Chai Feng Xu Yunfei Ding Qiang Zhu Zhaoxin Lu Kuan Shiong Khoo Xiaoying Bian Shujun Wang Pau Loke Show Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine biotechnology Jiangsu Ocean UniversityLianyungang 222005China Jiangsu Marine Resources Development Research Institute Lianyungang 222005China co.Innovation Center of Jiangsu Marine Bio-industry Technology Jiangsu Ocean UniversityLianyungang 222005China Helmholtz Institute of biotechnology State Key Laboratory of Microbial TechnologyShandong UniversityQingdao 266237China co.lege of Food Science and Technology Nanjing Agricultural UniversityNanjing 210095China Department of Chemical and Environmental Engineering Faculty of Science and EngineeringUniversity of Nottingham MalaysiaJalan Broga43500 SemenyihMalaysia

Skeletonema co.tatum is a diatom widely distributed in red tide microalgae blooms and as one of the main algae causing harmful algal blooms,because of their rapid reproduction and production of toxic and harmful substances,often play a negative role in aquatic eco.ystems,and human health and *** D is a nonribosomal cyclic antifungal lipopeptide in the iturins *** this study,Bacillomycin D was tested for its ability to inhibit the growth of *** EC_(50 24h) of Bacillomycin D on *** was 24.70μg/*** chlorophyll fluorescence parameters F_(v)/F_(m),F_(v)/F_(o),and yield of the diatoms decreased significantly with increasing co.centrations of Bacillomycin *** of the mechanism showed that Bacillomycin D induced cell death by changing cell membrane permeability,promoting the release of cellular *** this study,transcriptomic analysis showed Bacillomycin D significantly inhibited the photosynthesis and metabolism of *** findings investigated the inhibitory effect of Bacillomycin D on the growth of *** and provided a theoretical foundation for the development of new environmentally friendly biological algicide.

关键词： Bacillomycin D Skeletonema co.tatum Transcriptome Growth inhibition Photosynthesis

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The ornithine-urea cycle involves fumaric acid biosynthesis in Aureobasidium pullulans ***,a green and eco.friendly process for fumaric acid production

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Synthetic and Systems biotechnology 2023年第1期8卷 33-45页

作者： Xin Wei Miao Zhang Guang-Yuan Wang Guang-Lei Liu Zhen-Ming Chi Zhe Chi co.lege of Marine Life Sciences Ocean University of ChinaYushan RoadNo.5QingdaoChina Laboratory for Marine Biology and biotechnology Qingdao National Laboratory for Marine Science and Technology266003China co.lege of Life Science Shandong Province Key Laboratory of Applied MycologyQingdao Agricultural UniversityQingdao266109China

The current petroleum chemical methods for fumaric acid production can cause heavy pollution and global *** this study,the engineered strains of *** *** were found to be suitable for green fumaric acid *** and co.plementation of the relevant genes showed only the ornithine-urea cycle(OUC)was involved in high level fumarate biosynthesis which was co.trolled by the Ca^(2+)signaling *** of both the GOX gene enco.ing gluco.e oxidase and the PKS1 gene enco.ing the polyketide synthase for 3,5-dihydroxydecanoic acid biosynthesis and overexpression of the PYC gene enco.ing pyruvate carboxylase made the strain e-PYC produce 88.1±4.3 g/L of fumarate at flask level and 93.9±0.8 g/L of fumarate during the fed-batch *** a yeast-like fungal strain,it was very easy to cultivate *** *** DH177 and their mutants in the bioreactor and to edit its genomic DNAs to enhance fumarate *** was found that 2 mol of co.(2) co.ld be fixed during a maximal theoretical yield of 2 mol of fumarate per mole of gluco.e co.sumed in the ***,the OUC-mediated fumarate biosynthesis pathway in *** *** was a green and eco.friendly process for the global sustainable development and carbon neutrality.

关键词： Fumaric acid A.pullulans var.aubasidani Ornithine-urea cycle Ca^(2+)signaling pathway

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Redesigning transcription factor Cre1 for alleviating carbon catabolite repression in Trichoderma reesei

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Synthetic and Systems biotechnology 2020年第3期5卷 230-235页

作者： Lijuan Han Kuimei Liu Wei Ma Yi Jiang Shaoli Hou Yinshuang Tan Quanquan Yuan Kangle Niu Xu Fang State Key Laboratory of Microbial Technology Shandong UniversityQingdao266237China Rongcheng Campus Harbin University of Science and TechnologyWeihai264300China shandong Henglu Biological Technology co. LtdJinan250000China

Carbon catabolite repression(CCR),which is mainly mediated by Cre1 and triggered by gluco.e,leads to a decrease in cellulase production in Trichoderma *** studies have focused on modifying Cre1 for alleviating *** on the homologous alignment of CreA from wild-type Penicillium oxalicum 114–2(Po-0)and cellulase hyperproducer JUA10-1(Po-1),we co.structed a C-terminus substitution strain—Po-2—with decreased transcriptional levels of cellulase and enhanced *** revealed that the C-terminal domain of CreAPo−1 plays an important role in alleviating ***,we replaced the C-terminus of Cre1 with that of CreAPo−1 in ***(Tr-0)and generated *** a co.trol,the C-terminus of Cre1 was truncated and Tr-2 was *** transcriptional profiles of these transformants revealed that the C-terminal chimera greatly improves cellulase transcription in the presence of gluco.e and thus upregulates cellulase in the presence of gluco.e and weakens CCR,co.sistent with truncating the C-terminus of Cre1 in ***,we propose co.structing a C-terminal chimera as a new strategy to improve cellulase production and alleviate CCR in the presence of gluco.e.

关键词： Carbon catabolite repression Chimera Cre1 cel7a Trichoderma reesei

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The ontogeny of acidic digestive tract in larval turbot Sco.hthalmus maximus based on H+/K+-ATPase and pepsinogen

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Journal of Oceanology and Limnology 2023年第3期41卷 1100-1108页

作者： Yanfeng WANG Shulan LIU Teng GUO Shihong XU Qinghua LIU Zhizhong XIAO Daoyuan MA Jun LI Liang CHI Key Laboratory of Experimental Marine Biology Centre for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdao 266071China Qingdao Agricultural University Qingdao 266109China Laboratory for Marine Biology and biotechnology Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Southern Marine Science and Engineering Guangdong Laboratory Guangzhou 511458China co.son Oriental(Yantai)Recirculating Aquaculture Technology co. Ltd.Yantai 264000China

Acidic digestion is an important digestive process of marine *** fish stomach,two enzymes are involved in the secretion of hydrochloric acid(HCl)and proteomic digestion:H^(+)/K^(+)-ATPase and ***,the starting of digestive function in fish is still *** reveal the details of acidic digestion of turbot Sco.hthalmus maximus in early development,a 40 day of turbot larvae culture was *** H^(+)/K^(+)-ATPase gene from the turbot ***(smH^(+)/K^(+)-ATPase)was identified and *** on our previous discription on pepsinogen of turbot ***,we co.bined pepsinogen and H^(+)/K^(+)-ATPase and analyzed the mechanism of acidic digestion in *** show that the spatial and temporal expression profiles of H^(+)/K^(+)-ATPase agreed with pepsinogen A and C in turbot,indicating a synergetic action between H^(+)/K^(+)-ATPase and pepsinogen during the acidic digestion *** addition,the turbot juveniles showed a faster growth after the expressions of H^(+)/K^(+)-ATPase gene and pepsinogen gene,demonstrating that pepsin had a higher digestive efficiency,for which a co.pound diet should be provided to the fish from Day 22 *** study provided a reference for biology research and aquaculture of turbot and other marine fishes.

关键词： turbot Sco.hthalmus maximus acidic digestion H+/K+-ATPase pepsinogen

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Evaluation of hydrogels for soft tissue adhesives in vitro and in vivo analyses

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Frontiers of Materials Science 2018年第1期12卷 95-104页

作者： Liu YUAN Wenshuai FAN Linyingjun HAN Changan GUO Zuoqin YAN Meifang ZHU Xiumei MO State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and Engineering Donahua Universitv. Shanahai 201620. China Biomaterials and Tissue Engineering Lab College of Chemistry Chemical Engineering and Biotechnology Donghua University Shanghai 201620 China Department of Orthopedics Zhongshan Hospital of Fudan Universily 180 FengUn Road Shanghai 200032 China shandong International biotechnology Park Development co. Ltd. Yantai 264003 China

In this study, natural materials （sodium alginate, dextran, gelatin and carboxymethyl chitosan） were modified to get aldehyde co.ponents and amino co.ponents. Upon mixing the two-co.ponent solutions together, four kinds of Schiff base hydrogels formed successfully within 5-300 s and co.ld seal the wound tissue. The cytotoxicity tests of hydrogel extraction solution co.firmed that the hydrogels are nontoxic materials. The adhesive ability was evaluated in vivo by measuring the adhesive strength after sealing the skin incisions on the back of rats. All the hydrogels showed higher adhesive strength than that of co.mercial fibrin glue and the blank co.trol. The histological staining observation by hematoxylin and eosin staining （HE） and Masson＇s trichrome staining （MTC） methods suggested that the hydrogels had good bioco.patibility and biodegradation in vivo. They have only normal initial inflammation to skin tissue and co.ld improve the formation of new co.lagen in the incision section. So, the prepared hydrogels were both safe and effective tissue adhesive, which had the great potentials to be used as skin tissue adhesive.

关键词： Schiff's base injectable hydrogels tissue adhesive

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Detection of Patulin in Dairy Products by ELISA

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Animal Husbandry and Feed Science 2018年第2期10卷 142-144页

作者： Tang Shiyun Shen Zhiqiang Fu Shijun Wu Yuxiang Li Jinlin shandong Binzhou Animal Science & Veterinary Medicine Academy Binzhou 256600 China shandong Lvdu biotechnology co. Ltd. Binzhou 256600 China

The co.tent of patulin in dairy products was detected by rapid ELISA assay. The pretreatment of samples was simple, with wide range of application. The standard product showed good linear relationship with the range of 0-5.4 μg/kg, and the linear relationship reached 0.999 3, with high sensitivity. It took shorter time, with a total time-co.sumption of 1 h. Moreover, it had good repeatability for analytical requirements.

关键词： ELISA Patulin Detection of dairy products

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