The ontogeny of acidic digestive tract in larval turbot Scophthalmus maximus based on H+/K+-ATPase and pepsinogen

作     者：Yanfeng WANG Shulan LIU Teng GUO Shihong XU Qinghua LIU Zhizhong XIAO Daoyuan MA Jun LI Liang CHI Yanfeng WANG;Shulan LIU;Teng GUO;Shihong XU;Qinghua LIU;Zhizhong XIAO;Daoyuan MA;Jun LI;Liang CHI

作者机构：Key Laboratory of Experimental Marine BiologyCentre for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdao 266071China Qingdao Agricultural UniversityQingdao 266109China Laboratory for Marine Biology and BiotechnologyPilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Southern Marine Science and Engineering Guangdong LaboratoryGuangzhou 511458China Conson Oriental(Yantai)Recirculating Aquaculture Technology Co.Ltd.Yantai 264000China 

出 版 物：《Journal of Oceanology and Limnology》 (海洋湖沼学报（英文）)

年 卷 期：2023年第41卷第3期

页      面：1100-1108页

核心收录：

学科分类：0908[农学-水产] 09[农学] 

基　　金：Supported by the National Key Research and Development Program(No.2018YFD0901204) the Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou)(No.GML2019ZD0402) the Major Agricultural Application Technology Innovation Project of Shandong Province(No.SD2019YY011) the Qingdao National Laboratory for Marine Science and Technology(No.2018SDKJ0502-2) the China Agriculture Research System(No.CARS-47),the Major Science and Technology for Scientific and Technological Innovation Projects(Shandong)(No.2019JZZY020710) the STS Project(Nos.KFZD-SW-106,ZSSD-019,2017T3017,2019T3022) 

主　　题：turbot Scophthalmus maximus acidic digestion H+/K+-ATPase pepsinogen 

摘      要：Acidic digestion is an important digestive process of marine *** fish stomach,two enzymes are involved in the secretion of hydrochloric acid(HCl)and proteomic digestion:H^(+)/K^(+)-ATPase and ***,the starting of digestive function in fish is still *** reveal the details of acidic digestion of turbot Scophthalmus maximus in early development,a 40 day of turbot larvae culture was *** H^(+)/K^(+)-ATPase gene from the turbot ***(smH^(+)/K^(+)-ATPase)was identified and *** on our previous discription on pepsinogen of turbot ***,we combined pepsinogen and H^(+)/K^(+)-ATPase and analyzed the mechanism of acidic digestion in *** show that the spatial and temporal expression profiles of H^(+)/K^(+)-ATPase agreed with pepsinogen A and C in turbot,indicating a synergetic action between H^(+)/K^(+)-ATPase and pepsinogen during the acidic digestion *** addition,the turbot juveniles showed a faster growth after the expressions of H^(+)/K^(+)-ATPase gene and pepsinogen gene,demonstrating that pepsin had a higher digestive efficiency,for which a compound diet should be provided to the fish from Day 22 *** study provided a reference for biology research and aquaculture of turbot and other marine fishes.