Cas12a-based on-site, rapid detection of genetically modified crops

作     者：Zhiqiang Duan Xiaoliang Yang Xingkun Ji Ying Chen Xiaomu Niu Anping Guo Jian‐Kang Zhu Feng Li Zhaobo Lang Hui Zhao Zhiqiang Duan;Xiaoliang Yang;Xingkun Ji;Ying Chen;Xiaomu Niu;Anping Guo;Jian-Kang Zhu;Feng Li;Zhaobo Lang;Hui Zhao

作者机构：Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off‐Season Reproduction RegionsInstitute of Tropical Bioscience and Biotechnology&San Ya Research InstituteChinese Academy of Tropical Agricultural SciencesHaikou 571101China Bellagen Biotechnology Co.Ltd.Jinan 250000China College of Life SciencesShandong Normal UniversityJinan 250014China Institute of Advanced Biotechnology and School of Life SciencesSouthern University of Science and TechnologyShenzhen 518055China Center for Advanced Bioindustry TechnologiesChinese Academy of Agricultural SciencesBeijing 100081China Hainan Yazhou Bay Seed LabSanyaHainan 572024China Shanghai Center for Plant Stress BiologyCAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 201602China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2022年第64卷第10期

页      面：1856-1859页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 0828[工学-农业工程] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：funded by Hainan Province's Major Scientific and Technological Project“Biosafety Preventionand Control in Southern Breeding Area"(ZDKJ202002) the researchwas also supported by the innovation platform for Academicians of Hainan Province 

主　　题：crops modified diagnosis 

摘      要：Genetically modified(GM)crops that impart herbicide resistance,improved tolerance to biotic and abiotic stresses,and enhanced yield or nutritional quality have been developed and grown worldwide(ISAAA Brief 55-2019:International Servicefor theAcquisition ofAgri-biotech Applications(ISAAA),2019).In response topublic concern on genetically modified organisms(GMOs),DNA-based and protein-based methods have been developed to detect GMOs infood/feed samples(Fraitureetal.,2015).These methods,however,require expensive equipment and technical expertise and are unsuitable for field diagnosis(DNA-based methods)or cannot detect non-coding sequences such as promoters or terminators(protein-based methods).