The ornithine-urea cycle involves fumaric acid biosynthesis in Aureobasidium pullulans ***,a green and eco-friendly process for fumaric acid production
作者机构：College of Marine Life SciencesOcean University of ChinaYushan RoadNo.5QingdaoChina Laboratory for Marine Biology and BiotechnologyQingdao National Laboratory for Marine Science and Technology266003China College of Life ScienceShandong Province Key Laboratory of Applied MycologyQingdao Agricultural UniversityQingdao266109China
出 版 物：《Synthetic and Systems Biotechnology》 (合成和系统生物技术（英文）)
年 卷 期：2023年第8卷第1期
页 面：33-45页
核心收录：
学科分类：081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术]
基 金：supported by Grant 31970058 from National Natural Science Foundation of China
主 题：Fumaric acid A.pullulans var.aubasidani Ornithine-urea cycle Ca^(2+)signaling pathway
摘 要：The current petroleum chemical methods for fumaric acid production can cause heavy pollution and global *** this study,the engineered strains of *** *** were found to be suitable for green fumaric acid *** and complementation of the relevant genes showed only the ornithine-urea cycle(OUC)was involved in high level fumarate biosynthesis which was controlled by the Ca^(2+)signaling *** of both the GOX gene encoding glucose oxidase and the PKS1 gene encoding the polyketide synthase for 3,5-dihydroxydecanoic acid biosynthesis and overexpression of the PYC gene encoding pyruvate carboxylase made the strain e-PYC produce 88.1±4.3 g/L of fumarate at flask level and 93.9±0.8 g/L of fumarate during the fed-batch *** a yeast-like fungal strain,it was very easy to cultivate *** *** DH177 and their mutants in the bioreactor and to edit its genomic DNAs to enhance fumarate *** was found that 2 mol of CO_(2) could be fixed during a maximal theoretical yield of 2 mol of fumarate per mole of glucose consumed in the ***,the OUC-mediated fumarate biosynthesis pathway in *** *** was a green and eco-friendly process for the global sustainable development and carbon neutrality.
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