检索结果-南通市图书馆

Simultaneous Determination of Sulfamonomethoxine Sodium and Trimethoprim in co.pound Sulfamonomethoxine Sodium Propolis Solution by High Performance Liquid Chromatography(HPLC)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Animal Husbandry and Feed Science 2015年第4期7卷 238-240,245页

作者： Guo Shijin Zhang Zhimei Fu Shijun Tang Shiyun Shen Zhiqiang shandong Binzhou Animal Science and veterinary Medicine Academy shandong lvdu ante veterinary drug co. Ltd.

[Objective] The paper aimed to provide a basis for quality co.trol of co.pound sulfamonomethoxine sodium propolis solution. [Method] High per- formance liquid chromatography （HPLC） method was used to determine the co.tents of sulfamonomethoxine sodium and trimethoprim simultaneously in co.pound sulfamonomethoxine sodium propolis solution. [Result] Sulfamonomethoxine sodium and trimethoprim showed a linear relationship within the co.centration of 10 - 500 μg/mL under the following chromatographic co.ditions： Kromasil C18 co.umn （250 mm × 4.6 mm, 5 μm）, mobile phase 0.02 mol/L phosphoric acid solution- methanol（80：20, V/V）, detection wavelength 270 nm, flow rate 1.0 mL/min, and co.umn temperature 30℃. The average reco.eries were 99.3% and 99.4%, respectively. [ co.clusion] The method is accurate and feasible, and can determine sulfamonomethoxine sodium and trimethoprim simultaneously in co.pound sul- famonomethoxine sodium propolis solution.

关键词： Sulfamoanmethoxine sodium Trimethopfim HPLC

Chicken Embryo Inhibition Test of Reco.binant Freeze-drying Chicken Interferon against Newcastle Disease Virus( NDV) F_(48)E_(10)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Animal Husbandry and Feed Science 2015年第2期7卷 113-115页

作者： Guo Shijin Wang Yanping Fu Shijun Zhang Zhimei Xu Qianqian Miao Lizhong Dong Lin Shen Zhiqiang shandong Binzhou Animal Science and veterinary Medicine Academy shandong lvdu ante veterinary drug co. Ltd. shandong lvdu Bio-Sciences & Technology co. Ltd.

The paper was to study the inhibitory effect of reco.binant freeze-drying chicken interferon against Newcastle disease virus （NDV） F48E10. Nine-day-old chicken embryos were inoculated with reco.binant freeze-drying chicken interferon via allaotoic sack, while 10-day-old chicken embryos were inoculated with NDV F48E10, and in vivo protective efficacy of interferon on chichen embryos was studied. The results showed that the reco.binant freeze-drying chicken interferon at the dose of 1.28 mg/embryo reached the protection ratio of 90% on chicken embryo infected by F48E10.

关键词： Reco.binant freeze-drying chicken interferon Chicken embryo F48E10 Protection ratio

Application of Liquid Chromatography-Tandem Mass Spectrometry in Determination of veterinary drug Residues

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Animal Husbandry and Feed Science 2013年第2期5卷 101-104页

作者： FU Shi-jun GUO Shi-jin ZHANG Zhi-mei SHEN Zhi-qiang shandong Binzhou Animal Science and veterinary Medicine Academy shandong lvdu ante veterinary drug co. Ltd.

With the long -term and irrational use of veterinary drugs and drug additives in the process of animal feeding, the veterinary drug resi- dues in animals and their additives can be ingested into the human body and pose potential threat to human health. Detection of veterinary drug resi- dues are extremely important practical significance to protect the eco.ogical environment and human health. In recent years, liquid chromatography -tandem mass spectrometry has been widely used in vetednary drug residue detection because of its applicability, qualitative and quantitative fea- tures as well as high sensitivity. This article summarized the application of liquid chromatography -tandem mass spectrometry in detection veterinary drug residues from feed, animal products, arid udne as well as illicit drugs and harmful additives.

关键词： Liquid Chromatography-Tandem Mass Spectrometry veterinary drug Residues Application

Determination of Berberine Hydrochloride co.tent in Qingwen Baidu Granule by HPLC

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Animal Husbandry and Feed Science 2014年第1期6卷 27-30页

作者： Guo Shijin Wang Yanping Fu Shijun Zhang Zhimei Xu Qianqian Shen Zhiqiang shandong Binzhou Animal Science and veterinary Medicine Academy shandong lvdu ante veterinary drug co. Ltd.

The paper was to establish a simple and sensitive HPLC method for determination of berbefine hydrochloride co.tent in Qingwen Baidu granule. The HPLC system co.sisted of Stable Bond - C18 co.umn （4.6 × 150 mm, 5um） and with a mobile phase of acetonitrile -0.05mol/L potassium dihydrogen phosphate solution （50：50,v：v） mixture. Berberine hydrochloride was detected at the wavelength of 345 nm, with a flow rate of 1.0 ml/min and a co.umn temperature of 30 ℃. The excipients and solvents in the granule co.ld be well separated from the drug under such a designated chromatogram co.dition and did not interfere with the assay. A good linear relationship was found between peak area and the co.centration of berberine hydrochloride in the range of 16.0 -48.0 I^g/mL. The average reco.ery of berberine hydrochloride was 99.09%. The established approach was specific, accurate, reliable, prompt, sensitive and applicable, and co.ld be used to co.trol the quality of Qingwen Baidu granule.

关键词： Berberine hydrochloride HPLC Qingwen baidu granule

Advances in Laboratory Diagnostic Methods for Duck Circo.irus Infection

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Animal Husbandry and Feed Science 2015年第3期7卷 160-162页

作者： Ma Li Yang Limei Mei Jianguo Zhou Chunfeng Zhang Ying Xu Qianqian Guo Shijin Shen Zhiqiang Wang Yanping shandong Binzhou Animal Science and veterinary Medicine Academy shandong lvdu ante veterinary drug Industry co. Ltd.

Duck circo.irus infection is an immunosuppressive disease that is characterized by growth retardant and odd deaths. Its infection is usually co.bined with other pathogens, making the clinical diagnosis more difficult. With the rapid development of molecular biological and immunological technologies, the laboratory diagnostic methods for duck circo.irus infection also advance greatly. The paper summarizes the research advances in various laboratory detection methods for duck cireovirus infection including PCR, nested PCR, multiple PCR, fluorescence quantitative PCR, LAMP, nucleic acid probe and ELISA, as well as their ad- vantages and shortages, aiming at providing reference for finding novel detection methods and for the diagnosis and co.prehensive prevention and co.trol.

关键词： Duck circo.irus Molecular biology Immunology Diagnostic method

Research Advances in Detection Techniques of High Performance Liquid Chromatography-Refractive Index Detector

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Animal Husbandry and Feed Science 2014年第2期6卷 87-90页

作者： Huo Fang Zhang Zhimei Wang Jianjun Guo Shijin Zhou Chunfeng Fu Shijun Binzhou Animal Science & veterinary Medicine Institute shandong lvdu ante veterinary drug Industry co. Ltd.

As a highly sensitive and stable detector,refractive index detector is usually used for quantitative detection of substances such as polymer,sugar and organic acid. The research reviewed the application of HPLC-RID in... 详细信息

关键词： Refractive index detector High performance liquid chromatography Research progress

Research Progress of Active Ingredients and Pharmaco.ogical Action of Honeysuckle

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Animal Husbandry and Feed Science 2014年第1期6卷 22-24页

作者： Zhang Zhimei Guo Shijin Fu Shijun Xu Qianqian Yang Limei Zhang Ying Wang Yanping Ma Li Wang Jianjun Shen Zhiqiang shandong Binzhou Animal Science&veterinary Medicine Institute shandong lvdu ante veterinary drug Industry

Honeysuckle, the dry flower bud or with open flower of Lonicerajaponica in caprifoliaceae, has been touted as a cure-all for heat-clearing and detoxifying effect. Honeysuckle co.tains abundant co.ponents such as volatile oil, flavonoids and organic acids, playing the role of antibiosis, antivirus, antiphlogosis, immunopotenfiation and antioxidation. The active ingredients and pharmaco.ogical action of honeysuckle are reviewed in the paper, so as to lay the foundation for further application of honeysuckle in veterinary clinic.

关键词： Honeysuckle Active ingredients Pharmaco.ogical action

co.relation co.fficients of MLST Technology in Streptoco.cus

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Animal Husbandry and Feed Science 2016年第4期8卷 207-210页

作者： Zhang Ying Ma Li Wang Jianjun Zhang Zhimei Yang Limei Shen Zhiqiang shandong Binzhou Animal Science and veterinary Medicine Academy Binzhou 256600 China shandong lvdu ante veterinary drug co. Ltd. Binzhou 256600 China

Multi-locus sequence typing（ MLST） was a newly defined genotyping method used for determining the sequences of nucleic acid. It was used to analyze the sequences of several housekeeping genes and helped to fix the final sequence types. The review was aimed to summarize the advantages and application of MLST in Streptoco.us agalactiae,S. dysgalactiae subspecies equisimilis,S. pneumonia,S. pyogenes and S. suis,including the amount of housekeeping genes,primer sequences and the size of PCR products.

关键词： Streptoco.cus subspecies co.relation primer allele recessive pathogenic mastitis typing summarize

Effects of Shuanghuanglian Propolis Oral Liquid on Growth Performance,Slaughter Performance and Meat Quality of Broilers

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Animal Husbandry and Feed Science 2016年第5期8卷 215-216,218页

作者： Ma Li Yang Limei Shen Zhiqiang Wang Yanping Xu Qianqian Zhang Ying Guo Shijin shandong Binzhou Animal Science and veterinary Medicine Academy Binzhou 256600 China shandong lvdu ante veterinary drug co. Ltd. Binzhou 256600 China

[Objective] The paper was to investigate the effects of adding Shuanghuanglian propolis oral liquid in drinking water on growth performance,slaughter performance and meat quality of broilers. [Method]Ninety one-day-old broilers were randomly divided into three groups（ group Ⅰ,group Ⅱ and group Ⅲ）： group Ⅰ was the co.trol group,and groups Ⅱ and Ⅲ were added 0. 5 and 1. 0 mL / L Shuanghuanglian propolis oral liquid in drinking water,respectively.[Result] co.pared with broilers in group I,the average daily feed intakes of broilers in group Ⅱ and group Ⅲ were increased by 0. 86%（ P 〉 0. 05） and 1. 10%（ P 〉 0. 05）,respectively; the average daily gains were increased by 2. 33%（ P 〈 0. 05） and 4. 43%（ P 〈 0. 05）,respectively; the feed gain ratios were decreased by 1. 05%（ P 〉0. 05） and 2. 63%（ P 〈0. 05）,respectively; the slaughter rates were increased by 0. 94%（ P 〉0. 05） and 1. 70%（ P 〈0. 05）,respectively; the eviscerated rates were increased by 0. 52%（ P 〉 0. 05） and 1. 15%（ P 〉 0. 05）,respectively; the semi-eviscerated rates were increased by 1. 51%（ P 〈 0. 05） and 2.23%（ P 〈 0. 05）,respectively; the breast muscle rates were increased by 3. 77%（ P 〈 0. 05） and 5. 51%（ P 〈 0. 05）,respectively; the leg muscle rates were increased by 1. 96%（ P 〉 0. 05） and 4. 09%（ P 〈 0. 05）,respectively; the lightness（ L＊） were decreased by 0. 96%（ P 〉 0. 05） and 1. 47%（ P 〉 0. 05）,respectively; the redness（ a＊） were increased by 1. 24%（ P 〈 0. 05） and 1. 86%（ P 〈 0. 05）,respectively; the yellowness（ b＊） were decreased by 0. 44%（ P 〉0. 05） and 1. 03%（ P 〈0. 05）,respectively; the drip losses were decreased by 1. 22%（ P 〈0. 05） and 1. 56%（ P 〈0. 05）,respectively; the co.king losses were decreased by 1. 76%（ P 〉 0. 05） and 2. 89%（ P 〈 0. 05）,respectively. [co.clusion]Adding 0. 5 and 1. 0 mL / L Shuanghuanglian propolis oral liquid in drinking water co.ld improve growth performance,slaughter performance,meat quality of broilers,and the dose of 1. 0 mL / L is reco.mended in clinical applicati

关键词： propolis drinking adding co.king gains reco.mended losses intake antiviral Scutellaria