Determination of Berberine Hydrochloride Content in Qingwen Baidu Granule by HPLC

作     者：Guo Shijin Wang Yanping Fu Shijun Zhang Zhimei Xu Qianqian Shen Zhiqiang 

作者机构：Shandong Binzhou Animal Science and Veterinary Medicine Academy Shandong Lvdu Ante Veterinary Drug Co. Ltd. 

出 版 物：《Animal Husbandry and Feed Science》 (动物与饲料科学（英文版）)

年 卷 期：2014年第6卷第1期

页      面：27-30页

学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：Supported by Technology Innovation Project of Shandong Province(201210916001) 

主　　题：Berberine hydrochloride HPLC Qingwen baidu granule 

摘      要：The paper was to establish a simple and sensitive HPLC method for determination of berbefine hydrochloride content in Qingwen Baidu granule. The HPLC system consisted of Stable Bond - C18 column （4.6 × 150 mm, 5um） and with a mobile phase of acetonitrile -0.05mol/L potassium dihydrogen phosphate solution （50：50,v：v） mixture. Berberine hydrochloride was detected at the wavelength of 345 nm, with a flow rate of 1.0 ml/min and a column temperature of 30 ℃. The excipients and solvents in the granule could be well separated from the drug under such a designated chromatogram condition and did not interfere with the assay. A good linear relationship was found between peak area and the concentration of berberine hydrochloride in the range of 16.0 -48.0 I^g/mL. The average recovery of berberine hydrochloride was 99.09%. The established approach was specific, accurate, reliable, prompt, sensitive and applicable, and could be used to control the quality of Qingwen Baidu granule.