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Effects of Cryofrequency in the Different Swine Skin Tissue Layers: <i>In Vitro</i>and <i>in Vivo</i>Study

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Journal of Biosciences and Medicines 2021年第3期9卷 94-104页

作者： Rafaella Rêgo Maia Patrícia Froes Meyer Josebel da Costa Emerenciano Joseela Joyce Barbosa da Silva Júlio Davi Costa e Silva Liliane Santos de Vasconcellos Rodrigo Marcel Valentim da Silva Tarciso Bruno Montenegro Sampaio Ciro Dantas Soares Federal universityof Rio Grande do Norte (UFRN) Natal Brazil university Centre of Rio Grande do Norte (UNI-RN) Natal Brazil potiguar university (unp) Natal Brazil Maurício de Nassau College Natal Brazil universityof Campinas (UNICAMP) S&atilde o Paulo Brazil

Introduction: Cryofrequency is a novel therapy which was developed with the combination of cold (cryotherapy) and heat (radiofrequency) application, causing a thermal shock effect on skin tissue. The thermal shock is believed to alter local metabolism. Objective: To investigate the effect of cryofrequency on the skin tissue of a mini pig in an in vitro and in vivo experimental model. Methodology: A small swine of the S. domesticus species, a male mini-pig (30 kg weight) was elected for the in vivo procedure subject. The in vitro step was performed on the extracted abdominal part of another subject of the same species and breed. Then, after the application of cryofrequency in the monopolar and bipolar form in vivo, a histological analysis was performed. Results: A higher temperature variation was observed comparing the pilot study in vitro with respect to the experimental one in vivo. Microscopically, an increase of collagen fibers and blood supply and decreased adipose tissue was observed after cryofrequency application. Conclusion: With the bipolar cryofrequency, no reduction of adipose tissue was observed, but regarding the connective tissue, extensive areas of collagen deposition were observed, as well as a great amount of new-formed collagen fibers.

关键词： Cryotherapy Radio Frequency Adiposity Collagen Temperature

Effects of Tecar Therapy on Adipose Tissue: Clinical Trial

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Journal of Biosciences and Medicines 2022年第4期10卷 169-180页

作者： Patrícia Froes Meyer Rodrigo Marcel Valentim Da Silva Eneida De Morais Carreiro Fábio dos Santos Borges Stephany Queiroga Felice Picariello Centro Universitario do Rio Grande do Norte UNI-RN Natal Brazil Universidade Federal do Rio Grande do Norte UFRN Natal Brazil Estácio de Sa university Rio de Janeiro Brazil potiguar university UnP Natal Brazil university of the Study of Naples Federico II Nápoles Itália

Introduction: Localised adiposity is one of the leading aesthetic alteration complaints. Tecar therapy has been used in clinical practice for the treatment of fat located in the abdominal region. Objective: The objective is to analyse the effect of Tecar therapy on abdominal adiposity. Methodology: This is a clinical trial in which the participants were 32 female volunteers who have flaccidity and fat located in the abdominal region. The volunteers were divided into three groups, which respectively received applications of the capacitive mode, the resistive mode, and combined therapy of the two modalities. We have performed ten applications and analysed the anthropometric measures, the ultrasonography of the adipose layer, and the level of satisfaction. Results: All groups presented a reduction in perimetry compared to the baseline (p = 0.001). The ultrasonography showed a significant reduction in the combined therapy group compared to the baseline (p = 0.04 and p = 0.03). The level of satisfaction was good in all groups, with few adverse responses. In the bodyweight and percentage of fat analyses, we observed an increase in measurements, probably due to the restriction measures related to the new coronavirus pandemic in 2020. Conclusion: In this present study, we have concluded that Tecar therapy resulted in a reduction of the fat located in the abdominal region.

关键词： Radiofrequency Abdominal Fat Ultrasonography

Effects of Semi-Ablative Fractional Radiofrequency Associated with Growth Factors in Stretch Marks: A Randomized Controlled Trial

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Open Journal of Applied Sciences 2021年第4期10卷 379-394页

作者： Patricia F. Meyer Rodrigo Marcel V. Da Silva Liliane S. Vasconcelos Louhanne Christine B. Bezerra Maria Paula M. Oliveira Eneida M. Carreiro Ciro D. Soares Fabio S. Borges Monica Etrusco Joyce Rodrigues Silene B. Ribeiro Hernan Andrés B. Ortiz Federal university of Rio Grande do Norte (UFRN) Natal Brazil Physiotherapy Course at UNIRN Natal Brazil Mauricio de Nassau Natal Natal Brazil Physiotherapy Course at potiguar university (unp) Natal Brazil potiguar university (unp) Natal Brazil Graduate Program in Stomatopathology State University of Campinas S&#227 o Paulo Brazil Postgraduate Program in Dermatofunctional Physiotherapy Estácio de Sá University Rio de Janeiro Brazil Minas Gerais Faculty of Medical Sciences Minas Gerais Brazil Sã o Judas Tadeu university Butant&#227 Brazil FSG Centro Universitário Caxias do Sul Brazil Physiotherapy Course Escuela de Kinesiología Facultad de Ciencias de la Rehabilitación Universidad Andrés Bello Santiago Chile

Background: To investigate the effects of the association of semi-ablative fractional radiofrequency (RFF) with growth factors in stretch marks. Methods: This is a randomized controlled trial. The sample consisted of 28 female patients with complaints of gluteus or abdomen striae and divided into 2 equitable groups, named G1 (semi-ablative RFF without active principle association) and G2 (semi-ablative RFF with active principle association), being subdivided into G-1A and G-2A (abdominal applications) and G-1B and G-2B (gluteus applications). A photographic and histological analysis was performed using questionnaires about inflammation, presence of adverse effects and satisfaction of volunteers. Photographic analysis showed a reduction in the amount of striae alba in all groups. Results: The histological analysis showed an increase in fibroblast activity, collagen type I, and blood vessels, especially in the groups that associated the RFF with the active principle. In the inflammation analysis, the groups that performed intervention with active ingredients showed lower inflammatory signal. All groups had a reasonable level of satisfaction after the interventions;however, the group that used active ingredients reported greater results. Conclusion: The association of semi ablative RFF with growth factors showed a reduction in clinical inflammatory signs, mainly in the first 15 days, as well as higher collagen production, verified in 30 days, with satisfactory clinical results in all groups.

关键词： Striae Distensae Collagen Wound Healing

Comparative Study of Collagen Stimulation Using Semi-Ablative and Non-Ablative Radiofrequency

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Journal of Biosciences and Medicines 2022年第6期10卷 79-89页

作者： Patrícia Froes Meyer Rodrigo Marcel Valentim da Silva Eneida De Morais Carreiro Rafaella Rêgo Maia Stephany Luanna Queiroga Farias Ciro Dantas Soares Yuri Alexander Afonso Gustavo Felipe Pasqual Silene Bazi Ribeiro Roberto Tiago Alves Pinheiro university Centre of Rio Grande do Norte (UNIRN) Natal Brazil Estácio de Sá university Natal Brazil Federal university of Rio Grande do Norte Natal Brazil potiguar university (unp) Natal Brazil Campinas State university (UNICAMP) Campinas Brazil Brazilian Society of Plastic Surgery Brazil FSG university Centre of Serra Gaúcha Caxias do Sul Brazil MARC Institute Miami USA

Objective: Comparison between semi-ablative and non-ablative radiofrequency for collagen stimulation. Method: Three volunteers with scheduled surgical procedures of abdominoplasty were selected. After the evaluation, the abdominal area was divided into three quadrants, with the right region receiving monopolar non-ablative radiofrequency (RF) application. In contrast, the left region received fractionated radiofrequency (FRF) application, using three different needle sizes (0.5, 1.5, and 3.0 mm), and the intermediate area was kept as a control. After 24 hours, surgery was performed, and the skin flaps were collected for histological and immunohistochemical analyses. Results: An increase in total collagen and type I collagen was observed in the treated groups. There was also an increase in the expression of COX-2, CD68 positive cells (macrophages), and lymphocyte markers (CD3, CD20, and NK-CD57). Furthermore, it was seen that only the use of 3.0-mm needles kept the channels open in the superficial tissue after 24 hours. There were no significant differences between the modalities. Conclusion: Non-ablative and sub-ablative radiofrequency have positive and effective results for flaccidity, demonstrating the success in collagen production. Furthermore, this study is the first to present channel opening and permanence time, which are important to optimize the action of drug delivery.

关键词： Collagen Flaccidity Radiofrequency Drug Delivery

Effect of Microfocused Ultrasound on Facial Rejuvenation: Clinical and Histological Evaluation

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Journal of Biosciences and Medicines 2021年第7期9卷 112-125页

作者： Patricia Froes Meyer Michelle Meleck Fabio dos Santos Borges Esteban Fortuny Stephany Luanna Queiroga Farias Felipe Alexander Caldas Afonso Ciro Dantas Soares Eneida de Morais Carreiro Rodrigo Marcel Valentim da Silva André Luiz Miranda Barbosa Federal university of Rio Grande do Norte (UFRN) Natal Brazil university Centre of Rio Grande do Norte (UNI-RN) Natal Brazil Michelle Meleck Advanced Aesthetics S&atilde o Paulo Brazil Estácio de Sá university Rio de Janeiro Brazil Universidad Finis Terrae Santiago Chile potiguar university (unp) Natal Brazil Campinas State university (UNICAMP) Campinas Brazil Fellow da West Virginia university Morgantown USA

Introduction: Micro focused ultrasound (MFU) is formed by mechanical waves emitted in a focused manner, concentrating thermal energy on a certain point or region, promoting a clinical improvement in wrinkles. Objective: evaluate the effects of micro focused ultrasound on facial rejuvenation. Methodology: This is an experimental study. The sample consisted of 30 volunteers, who were evaluated before treatment, 45 and 90 days after its beginning. A single application of the MFU was performed across the facial region. The parameters used were: 10 MHz cartridge with depth of 1.5 mm, 7 MHz with depth of 3 mm, and 4 MHz with focal depth of 4.5 mm;doses ranged from 0.1 to 2.0 J, according to the sensitivity of the volunteers treated, and the application time was of approximately 90 minutes. Clinical photographic analysis and facial symmetry analysis using Dolphin Imaging software were performed, and validated questionnaires. Two volunteers were submitted to blepharoplasty surgery, and one to rhytidoplasty surgery 45 days after the application of the MFU. Samples of the treated skin were taken after the surgical procedures and submitted to histological and immunohistochemical analyses. The volunteers answered a questionnaire to assess adverse reactions and satisfaction. Results: We observed a visible clinical improvement on the images by photography, and an improvement of facial symmetry in the measurements of the paralateronasal projection (p = 0.007), left lateral mandibular (p = 0.01), and right lateral mandibular (p = 0.008). The histological analysis showed an increase in the amount of fibroblasts (p = 0.02), blood vessels (p = 0.0062) and inflammatory cells (p = 0.0036), in addition to the production of type 1 collagen. In the immunohistochemical analysis, we observed the presence of the macrophage marker IHQ: CD68, indicating phagocytosis of adipose tissue and the presence of fibrosis. In the analysis of adverse reactions, only transient hyperemia was observed with t

关键词： Skin Aging Mechanical Waves Sagging Wrinkles

Endothelin and neonatal capsaicin regulate gastric resistance to injury in BDL rats

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World Journal of Gastrointestinal Pathophysiology 2012年第4期3卷 85-91页

作者： Paula RS Cmara Gerson JN Ferraz Licio A Velloso José Murilo R Zeitune Fernando AB Suassuna Jose Geraldo P Ferraz Department of Medicine Potiguar University GI Research Lab Gastrocentro Department of Medicine Faculty of Medical Sciences University of Campinas Division of Gastroenterology Faculty of Medicine University of Calgary

AIM: To investigate the relationship between primary afferent neurons, endothelin (ET) and the role of its receptors on ethanol-induced gastric damage in cirrhotic rats. METHODS: Cirrhosis and portal hypertension were induced in rats by bile duct ligation (BDL) while controls had a sham operation. The association between ET and afferent neurons on the gastric mucosa was evaluated by capsaicin treatment in newborn rats, the use of ET agonists or antagonists, gastric ET-1 and -3 mRNA and synthetic capacity. Ethanol-induced damage was assessed using ex vivo gastric chamber *** blood flow was measured by laser-Doppler flow-metry. RESULTS: ET-3 and an ETB receptor antagonist sig- nificantly reduced the extent of ethanol-induced gastric damage in BDL rats. Gastric ET-1 and -3 levels were 30% higher in BDL rats compared to control rats. Cap-saicin treatment restored the gastric resistance and blood flow responses to topical application of ethanol in BDL rats and ET-1 and -3 production to levels observed in controls. CONCLUSION: Our results suggest that the reduced resistance of the gastric mucosa of cirrhotic rats to ethanol-induced injury is a phenomenon modulated by ET through the ET B receptor and by sensory afferent neurons.

关键词： Endothelins Tachykinins Portal hypertension Gastropathy Cirrhosis

Effects of Hydrolysed Collagen Associated or Not with Physical Agents on Tissue and Muscle Flaccidity—Firmez Method: Case Study

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Open Journal of Applied Sciences 2022年第1期12卷 82-98页

作者： Rafaella Rêgo Maia Patrícia Froes Meyer Rodrigo Marcel Valentim Da Silva Eneida De Morais Carreiro Thaynar Gonzaga Da Silva Lígia De Oliveira Revorêdo Stephany Luanna Queiroga Farias Ciro Dantas Soares Michelle Cordeiro Sanz Mariana Adélia Girotto Bussade José Dos Santos Bussade Mônica Etrusco Pedrosa Federal university of Rio Grande do Norte Natal Brazil university Centre of Rio Grande do Norte (UNIRN) Natal Brazil Estácio de Sá university Natal Brazil potiguar university—unp Natal Brazil Campinas State university (UNICAMP) Campinas Brazil Higher School of Sciences of Santa Casa de Misericórdia Vitória Brazil university of Säo Judas Tadeu S&#228o Paulo Brazil Federal university of Rio de Janeiro Rio de Janeiro Brazil university of Medical Sciences of Minas Gerais Minas Gerais Brazil

The combination of therapies may be the promising future for the treatment of esthetic alterations more efficiently and in less time, in order to obtain an excellent result. Our objective was to evaluate the effectiveness of hydrolysed collagen associated with radiofrequency and electrostimulation in the treatment of flaccidity in the abdominal and gluteal regions. The sample consisted of 6 women, evaluated through evaluation protocols, questionnaires, dynamometry, ultrasound exams, and histological analysis. The volunteers were distributed into three subgroups: hydrolysed collagen group (G-1), radiofrequency + hydrolysed collagen group (G-2) and radiofrequency + hydrolysed collagen + electrostimulation group (G-3). The ultrasound analysis of the abdominal muscles showed an increase in muscle thickness in all groups, but not significant (p > 0.05). The histological analysis showed an evident increase in type I collagen in groups G-2 and G-3, with proliferating cells (Ki67+). The results of dynamometry showed significant values in different times (p < 0.05). There was a significant result in the infraumbilical perimetry analysis, when compared between the groups with 30 days (p = 0.03) and with 60 days (p = 0.04). All participants rated the treatment positively. The use of combined therapies has shown superior results for the treatment of abdominal and gluteal flaccidity when compared with its isolated use, being the combination of therapies important in the treatment of flaccidity.

关键词： Sagging Collagen Radiofrequency Electro-Stimulation

Left-Handed Students and Clinical Practice in Dentistry: Adaptations, Difficulties and Realities Experienced in the Academic Environment

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Open Journal of Preventive Medicine 2016年第11期6卷 247-259页

作者： Elisa Marianna Abreu Silva Itanielly Dantas Silveira Cruz Iris do Céu Clara Costa Kenio Costa de Lima Georgia Costa de Araújo Souza Maria Alice Pimentel Fuscella Fábia Barbosa de Andrade Odontology Department Federal University of Rio Grande do Norte Natal Brazil State university of Rio Grande do Norte Caicó Brazil Odontology Course Potiguar Faculty Natal Brazil Faculty of Health Sciences of Trairi Federal University of Rio Grande do Norte Santa Cruz Brazil

Objectives: To verify the existence of difficulties throughout the course of dentistry clinical practices, identify musculoskeletal pain, and evaluate frequency and intensity of pain/discomfort (if existent) in different regions of the body, experienced by left- handed students in comparison to right-handed students of the odontology courses of the state of Rio Grande do Norte (Northeast Brazil). Methods: A general questionnaire was applied to identify left-handed students in a population of 750 students, enrolled in clinical courses. An adaptation of the Grad-Corllet Diagram and a specific questionnaire on clinical practices were applied to the selected students. Descriptive statistics was utilized to analyze data of the specific questionnaire, and Mann-Whitney statistics, chi-square or Fisher’s Exact tests (with a 5% significance level) were utilized for the Grad-Corllet Diagram, searching for an association be- tween discomfort/pain in different body regions and manual laterality. Results: All students presented higher percentage of pain in the lumbar and neck/cervical re- gions. However, left-handed students presented higher values, with statistical signifi- cance levels of p = 0.041 for lower back, p = 0.002 for neck and p = 0.003 for cervical region. Conclusions: The majority of left-handed students classified “moderate” the difficulty of working with equipment designed for right-handers. There was high frequency of musculoskeletal pain/discomfort, with relevant statistical results for left- handed students regarding intensity. It is suggested that the participating institutions evaluate the adoption of inclusion politics, re-adapt the infrastructure to include equipment designed for left-handers, and finally provide adequate work conditions for all students.

关键词： Left-Hander Odontology Undergraduate Academic Orientation Musculoskeletal Pain

2015年巴西寨卡病毒感染与小头畸形之间可能存在的关联

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上海预防医学 2017年第1期29卷 82-84页

作者： Lavinia Schuler-Faccini Erlane M.Ribeiro Ian M.L.Feitosa Dafne D.G.Horovitz Denise P.Cavalcanti AndréPessoa Maria Juliana R.Doriqui Joao Ivanildo Neri Joao Monteiro de Pina Neto Hector Y.C.Wanderley Mirlene Cernach Antonette S.El-Husny Marcos V.S.Pone Cassio L.C.Serao Maria Teresa V.Sanseverino 朱秋丽(译) 陆仁敏(校) Universidade Federal do Rio Grande do Sul Brazil Hospital Infantil Albert Sabin FortalezaCEBrazil Universidade Federal de Pernambuco Brazil Fundaqao Oswaldo Cruz Rio de JaneiroBrazil university of Campinas Sao PauloBrazil Hospital Infantil Juvencio Mattos MaranhaoBrazil Universidade potiguar Rio Grande do NorteBrazil university of Sao Paulo Ribeirao PretoBrazil Secretaria de Estado da Saude do Espirito Santo Brazil Universidade Federal de Sao Paulo Brazil Centro Universitario do Estado do Para Brazil Universidade do Estado do Rio de Janeiro Brazil Hospital de Clinicas de Porto Alegre Brazil Brazilian Medical Genetics Society-Zika Embryopathy Task Force 不详

2015年初,通过伊蚊传播的寨卡病毒在登革热病毒流行地区巴西的东北部暴发,该病毒属于黄病毒属。2015年9月,寨卡病毒流行地区开始出现关于小头畸形患儿的报道,且报道数量呈现增加的趋势。与此同时,有2名妇女产前超声检查发现有胎儿畸形,... 详细信息

2015年初,通过伊蚊传播的寨卡病毒在登革热病毒流行地区巴西的东北部暴发,该病毒属于黄病毒属。2015年9月,寨卡病毒流行地区开始出现关于小头畸形患儿的报道,且报道数量呈现增加的趋势。与此同时,有2名妇女产前超声检查发现有胎儿畸形,在她们的羊水中也检测出寨卡病毒RNA。

关键词：小头畸形病毒感染报道数量登革热病毒产前超声检查胎儿畸形西寨黄病毒属病毒流行先天性异常