Effects of Cryofrequency in the Different Swine Skin Tissue Layers: <i>In Vitro</i>and <i>in Vivo</i>Study

作     者：Rafaella Rêgo Maia Patrícia Froes Meyer Josebel da Costa Emerenciano Joseela Joyce Barbosa da Silva Júlio Davi Costa e Silva Liliane Santos de Vasconcellos Rodrigo Marcel Valentim da Silva Tarciso Bruno Montenegro Sampaio Ciro Dantas Soares Rafaella Rêgo Maia;Patrícia Froes Meyer;Josebel da Costa Emerenciano;Joseela Joyce Barbosa da Silva;Júlio Davi Costa e Silva;Liliane Santos de Vasconcellos;Rodrigo Marcel Valentim da Silva;Tarciso Bruno Montenegro Sampaio;Ciro Dantas Soares

作者机构：Federal Universityof Rio Grande do Norte (UFRN) Natal Brazil University Centre of Rio Grande do Norte (UNI-RN) Natal Brazil Potiguar University (UNP) Natal Brazil Maurício de Nassau College Natal Brazil Universityof Campinas (UNICAMP) Sã o Paulo Brazil 

出 版 物：《Journal of Biosciences and Medicines》 (生物科学与医学（英文）)

年 卷 期：2021年第9卷第3期

页      面：94-104页

学科分类：0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学] 

主　　题：Cryotherapy Radio Frequency Adiposity Collagen Temperature 

摘      要：Introduction: Cryofrequency is a novel therapy which was developed with the combination of cold (cryotherapy) and heat (radiofrequency) application, causing a thermal shock effect on skin tissue. The thermal shock is believed to alter local metabolism. Objective: To investigate the effect of cryofrequency on the skin tissue of a mini pig in an in vitro and in vivo experimental model. Methodology: A small swine of the S. domesticus species, a male mini-pig (30 kg weight) was elected for the in vivo procedure subject. The in vitro step was performed on the extracted abdominal part of another subject of the same species and breed. Then, after the application of cryofrequency in the monopolar and bipolar form in vivo, a histological analysis was performed. Results: A higher temperature variation was observed comparing the pilot study in vitro with respect to the experimental one in vivo. Microscopically, an increase of collagen fibers and blood supply and decreased adipose tissue was observed after cryofrequency application. Conclusion: With the bipolar cryofrequency, no reduction of adipose tissue was observed, but regarding the connective tissue, extensive areas of collagen deposition were observed, as well as a great amount of new-formed collagen fibers.