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Effects of different plasma expanders on rats subjected to severe acute normovolemic hemodilution

Military Medical Research 2021年第2期8卷 183-195页

作者： Guo-Xing You Bing-Ting Li Zhen Wang Quan Wang Ying Wang Jing-Xiang Zhao Lian Zhao Hong Zhou institute of Health Service and transfusion medicine Beijing 100850China

Background:Plasma expanders are widely used for acute normovolemic hemodilution(ANH).However,existing studies have not focused on large-volume infusion with colloidal plasma expanders,and there is a lack of studies that compare the effects of different plasma ***:The viscosity,hydrodynamic radius(Rh)and colloid osmotic pressure(COP)of plasma expanders were determined by a cone-plate viscometer,Zetasizer and cut-off membrane,*** male rats were randomized into five groups with Gelofusine(Gel),Hydroxyethyl Starch 200/0.5(HES200),Hydroxyethyl Starch 130/0.4(HES130),Hydroxyethyl Starch 40(HES40),and Dextran 40(Dex40),with 12 rats used in each group to build the ANH *** was performed by the withdrawal of blood and simultaneous infusion of plasma ***-base,lactate,blood gas and physiological parameters were ***:Gel had a lower intrinsic viscosity than HES200 and HES130(P<0.01),but at a low shear rate in a mixture of colloids,red cells and plasma,Gel had a higher viscosity(P<0.05 or P<0.01,respectively).For hydroxyethyl starch plasma expanders,the COP at a certain concentration decreases from 11.1 mmHg to 6.1 mmHg with the increase of Rh from 10.7 nm to 20.2 nm.A severe ANH model,with the hematocrit of 40%of the baseline level,was established and accompanied by disturbances in acid-base,lactate and blood gas *** the end of ANH and 60 min afterward,the Dex40 group showed a worse outcome in maintaining the acid-base balance and systemic oxygenation compared to the other *** systolic blood pressure(SBP),diastolic blood pressure(DBP),and mean arterial pressure(MAP)decreased significantly in all groups at the end of *** DBP and MAP in the Dex40 group further decreased 60 min after the end of *** the process of ANH,the Dex40 group showed a drop and recovery in SBP,DBP and *** DBP and MAP in the HES200 group were significantly higher than those in the other groups at some time points(P<0.05 o

关键词： Acute normovolemic hemodilution Plasma expander Acid-base Lactate Blood gas Physiological arameters

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CAPE promotes the expansion of human umbilical cord blood-derived hematopoietic stem and progenitor cells in vitro

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Science China(Life Sciences) 2014年第2期57卷 188-194页

作者： LIU YiMing ZHANG BoWen ZHANG Jing WANG SiHan YAO HaiLei HE LiJuan CHEN Lin YUE Wen LI YanHua PEI XueTao Stem Cell and Regenerative medicine Lab Beijing Institute of Transfusion Medicine

Due to the low number of collectable stem cells from single umbilical cord blood(UCB)unit,their initial uses were limited to pediatric *** applications of UCB hematopoietic stem and progenitor cells(HSPCs)would become feasible if there were a culture method that can effectively expand HSPCs while maintaining their self-renewal *** recent years,numerous attempts have been made to expand human UCB HSPCs in *** this study,we report that caffeic acid phenethyl ester(CAPE),a small molecule from honeybee extract,can promote in vitro expansion of *** with CAPE increased the percentage of HSPCs in cultured mononuclear ***,culture of CD34+HSPCs with CAPE resulted in a significant increase in total colony-forming units and high proliferative potential colony-forming ***-forming unit-erythroid was the mostly affected colony type,which increased more than 3.7-fold in 1μg mL 1CAPE treatment group when compared to the *** appears to induce HSPC expansion by upregulating the expression of SCF and HIF1-α.Our data suggest that CAPE may become a potent medium supplement for in vitro HSPC expansion.

关键词： hematopoietic stem and progenitor cells caffeic acid phenethyl ester expansion

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Advances in cell lineage reprogramming

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Science China(Life Sciences) 2013年第3期56卷 228-233页

作者： ZHOU JunNian YUE Wen PEI XueTao Stem Cell and Regenerative medicine Lab Beijing Institute of Transfusion Medicine

As a milestone breakthrough of stem cell and regenerative medicine in recent years, somatic cell reprogramming has opened up new applications of regenerative medicine by breaking through the ethical shackles of embryonic stern cells. However, induced pluripo- tent stem （iPS） cells are prepared with a complicated protocol that results in a low reprogramming rate. To obtain differentiated target cells, iPS cells and embryonic stem cells still need to be induced using step-by-step procedures. The safety of induced target cells from iPS cells is currently a further concerning matter. More broadly conceived is lineage reprogramming that has been investigated since 1987. Adult stem cell plasticity, which triggered interest in stem cell research at the end of the last century, can also be included in the scope of lineage reprogramming. With the promotion of iPS cell research, lineage reprogramming is now considered as one of the most promising fields in regenerative medicine, will hopefully lead to customized, personalized therapeutic options for patients in the future.

关键词： regenerative medicine lineage reprogramming transdifferentiation dedifferentiation transdetemination

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Down-regulation of αGal epitopes by co-transfection of α1,3-galactosidase gene and α1,2-fucosyltransferase gene

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Chinese Science Bulletin 2005年第23期50卷 2723-2727页

作者： GONG Feng ZHANG Yangpei JIA Yanjun WANG Yingli TAN Yingxia TIAN shuguang Beijing institute of transfusion medicine Beijing 100850

The polycarbohydrate structure of Galα1- 3Galβ1-4GluNAc-R (known as αGal epitopes of xenoantigen), produced by α1-3-galactosyltransferase (α1,3-GT) in the course of animal development, is the major xenoantigen on the cell surface of porcine which causes hyperacute rejection in pig-to-human xenotransplantation. Alpha-1,3-galactosi- dase (AGL), a hydrolytic enzyme, can remove the terminal α-1,3-galactosyl from the Galα1-3Galβ1-4GluNAc-R struc-ture resulting in cleaning αGal epitopes from the porcine cells. Alpha-1,2-fucosyltransferase (HT) can modify the sur-face carbohydrate phenotype of porcine cells, bringing about reduction of αGal epitopes expression. In this study, human AGL and HT gene were co-transfected to porcine fetal fibro-blast (PFFb) in equimolar concentration to reduce the xeno-antigen. Gene and protein of hAGL and HT were both de-tected to express at high level by RT-PCR and Western blot, respectively. There was an 84% reduction in αGal xenoanti-gen and an 82% increase in H antigen as assayed by flow cytometry in the AGL and HT gene co-transfected PFFb. The number and morphology of transgenic PFFb chromosome were normal. Findings indicate that Galα1-3Gal epitopes of PFFb could be down regulated by AGL and HT co-transfec- tion without deleterious effects on the chromosomal profile of the transgenic cell.

关键词：异种移植术 αGal抗原决定基减量调节聚多糖 α1-3-半乳糖苷酶 α1-2-墨角藻糖基乳糖

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Microenvironment and cell mechanics

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BIOCELL 2022年第7期46卷 1629-1632页

作者： VAN-CHIEN BUI institute for Immunology and transfusion medicine University Medicine Greifswald17475GreifswaldGermany

Microenvironment contains biophysical and biochemical elements to maintain survival,growth,proliferation,and differentiation of *** change can lead to cell response to the mechanical forces,which can be described by *** is an indicator of a cell’s state since it plays an important role in many cellular *** many cases,cell elasticity is measured by using discontinuous manner,which may not allow elucidating real-time activity of individual live cells in physiological condition or cell response against microenvironmental changes.I argue that measuring cell elasticity using continuously repetitive nanoindentation technique is important that should be *** an example,I discuss mechanics of human embryonic kidney(HEK)cells in various *** resting cells,there is an activity of the cytoskeleton whose oscillation amplitude is strongly affected by the intracellular calcium,and the collective activity of myosin motor proteins induces elasticity *** results also reveal that actin cytoskeleton and cell membrane determine cell mechanics.

关键词： Microenvironment Cell mechanics Nanoindentation

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EXPRESSION OF HUMAN α-GALACTOSIDASE AND α1,2-FUCOSYL-TRANSFERASE GENES MODIFIES THE CELL SURFACE GALα1,3GAL ANTIGEN AND CONFERS RESISTANCETO HUMAN SERUM-MEDIATED CYTOLYSIS

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Chinese Medical Sciences Journal 2004年第1期19卷 31-37页

作者：贾延军任会明高新季守平杨军刘泽鹏李素波章扬培 institute of transfusion medicine Academy of Military Medical Sciences

Objective To explore the strategies which reduce the amount of xenoantigen Galα1, 3 Gal. Methods Human α-galactosidase gene and α1,2-fucosyltransferase gene were transferred into cul-tured porcine vascular endothelial cells PEDSV.15 and human α-galactosidase transgenic mice were produced. The Galα1,3Gal on the cell surface and susceptibility of cells to human antibody-mediated lysis were analyzed. Results Human α-galactosidase gene alone reduced 78% of Galα1,3Gal on PEDSV.15 cell surface while human α-galactosidase combined with α1,2-fucosyltransferase genes removed Galα1,3Gal completely. Decrease of Galα1,3Gal could reduce susceptibility of cells to human antibody-mediated lysis, especially during co-expression of α-galactosidase gene and α1,2-fucosyltransferase gene. RT-PCR indicated positive human α-galactosidase gene expression in all organs of positive human α-galacto-sidase transgenic F1 mice including heart, liver, kidney, lung, and spleen, the amount of Galα1,3Gal antigens on which was reduced largely. 58% of spleen cells from F1 mice were destroyed by comp-lement-mediated lysis compared with 24% of those from normal mice. Conclusions Human α-galactosidase gene and α1,2-fucosyltransferase gene effectively reduce the expression of Galα1,3Gal antigens on endothelial cell surface and confers resistance to human serum-mediated cytolysis. The expression of human α-galactosidase in mice can also eliminate the Galα1,3Gal antigens in most tissues and decrease the susceptibility of spleen cells to human serum-mediated cytolysis.

关键词： galactosidase gene hyperacute rejection Galα1,3Gal antigen

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Differentiation of human embryonic stem cells along a hepatocyte lineage and its application in liver regeneration

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Chinese Science Bulletin 2008年第1期53卷 1-7页

作者： PEI HaiYun WANG YunFang PEI XueTao Department of Stem Cell and Regenerative medicine Beijing Institute of Transfusion Medicine Beijing 100850 China

Hepatocyte transplantation and bioartificial liver (BAL) as alternatives to liver transplantation offer the possibility of effective treatment for many inherited and acquired hepatic disorders. Unfortunately, the limited availability of donated livers and the variability of their derived hepatocytes make it difficult to obtain enough viable human hepatocytes for the hepatocyte-based therapies. Embryonic stem cells (ESCs), which could be isolated directly from the blastocyst inner cell mass, have permanent self-renewal capability and developmental pluripotency and therefore might be an ideal cell source in the treatment of hepatic discords. However, differentiation of hESCs into hepatocytes with significant numbers remains a challenge. This review updates our current understanding of differentiation of ESCs into hepatic lineage cells, their future therapeutic uses and problems in liver regeneration.

关键词：胚胎干细胞肝细胞分化肝再生

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Differentiation potential of bone marrow mesenchymal stem cells into retina in normal and laser-injured rat eye

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Science China(Life Sciences) 2004年第3期47卷 241-250页

作者： ZHANG Jie1,2, SHAN Qing1, MA Ping1, JIANG Yanming1, CHEN Peng1, WEN Jingxia1, ZHOU You1, QIAN Huanwen1 & PEI Xuetao2 1. Beijing institute of Radiation medicine, Beijing 100850, China 2. Beijing institute of transfusion medicine, Beijing 100850, China 1. Beijing institute of Radiation medicine 100850 Beijing China 2. Beijing institute of transfusion medicine 100850 Beijing China

Bone marrow mesenchymal stem cells (MSCs) can develop into hematopoietic and mesenchymal lineages but have not been known to participate in the production of retina. Here we report that bone marrow mesenchymal stem cells, after being subretinally transplanted into normal or Nd: YAG laser-injured rat eye, can integrate into RPE layer, photoreceptor layer, bi-polar cell layer and ganglion layer. DAPI-labeling detection was used to trace the origin of the repopulating cells. DAPI fluorescence was used to identify retina cells of bone marrow origin 10, 20, 35 and 50 days after transplantation. No formation of rosettes was found but some random cells were found at the end of the observation. MSCs-originated cells spread more widely in the injured retinas than in the normal ones. Immunohistochemical detection showed that though the cells could express neuronal nuclei (NeuN), neuron specific enolase (NSE), glial fibrillary acidic protein (GFAP) and cytokeratin (CK), the proteins expression in the injured transplantation group was abnormal in some region compared with that in the normal transplantation group. Elec-troretinogram (ERG) showed that ERG-b wave of the injured transplantation group is significantly higher than that of the two laser-injured control groups. These results suggest that a proportion of MSCs can differentiate into retina-like structure in vivo and the differentiation differs in normal and laser-injured retinas.

关键词： MSCs, retina, laser, differentiation.

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3D genomic organization in cancers

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Quantitative Biology 2023年第2期11卷 109-121页

作者： Junting Wang Huan Tao Hao Li Xiaochen Bo Hebing Chen institute of Health Service and transfusion medicine Beijing 100850China

Background:The hierarchical three-dimensional(3D)architectures of chromatin play an important role in fundamental biological processes,such as cell differentiation,cellular senescence,and transcriptional *** chromatin 3D structural alterations often present in human diseases and even cancers,but their underlying mechanisms remain ***:3D chromatin structures(chromatin compartment A/B,topologically associated domains,and enhancerpromoter interactions)play key roles in cancer development,metastasis,and drug *** techniques based on machine learning and deep learning have shown great potential in the study of 3D cancer ***:Current advances in the study of the 3D cancer genome have expanded our understanding of the mechanisms underlying tumorigenesis and *** will provide new insights into precise diagnosis and personalized treatment for cancers.

关键词： the three-dimensional(3D)genome chromatin compartment topologically associated domain(TAD) loop cancer

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iPS cells—Alternative pluripotent cells to embryo stem cells

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Science China(Life Sciences) 2010年第1期53卷 154-156页

作者： PEI XueTao Beijing institute of transfusion medicine,Beijing 100850,China 1. Beijing institute of transfusion medicine Beijing 100850 China

Since the first murine and human embryonic stem cell lines were established by Drs. Evans and Kaufman [1] and Thomson et al. [2], respectively, great progress has been make in the field

关键词： iPS cells cell Alternative pluripotent cells to embryo stem cells stem

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