Differentiation of human embryonic stem cells along a hepatocyte lineage and its application in liver regeneration

作     者：PEI HaiYun WANG YunFang PEI XueTao 

作者机构：Department of Stem Cell and Regenerative Medicine Beijing Institute of Transfusion Medicine Beijing 100850 China 

出 版 物：《Chinese Science Bulletin》 (Chin. Sci. Bull.)

年 卷 期：2008年第53卷第1期

页      面：1-7页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：the National Key Project for Basic Research of China (Grant No. 2005CB522702) State 863 High Technology R&D Project of China (Grant No. 2006AA02A107) the National Natural Science Foundation of China (Grant No. 30671098) 

主　　题：胚胎干细胞 肝细胞 分化 肝再生 

摘      要：Hepatocyte transplantation and bioartificial liver (BAL) as alternatives to liver transplantation offer the possibility of effective treatment for many inherited and acquired hepatic disorders. Unfortunately, the limited availability of donated livers and the variability of their derived hepatocytes make it difficult to obtain enough viable human hepatocytes for the hepatocyte-based therapies. Embryonic stem cells (ESCs), which could be isolated directly from the blastocyst inner cell mass, have permanent self-renewal capability and developmental pluripotency and therefore might be an ideal cell source in the treatment of hepatic discords. However, differentiation of hESCs into hepatocytes with significant numbers remains a challenge. This review updates our current understanding of differentiation of ESCs into hepatic lineage cells, their future therapeutic uses and problems in liver regeneration.