Effects of different plasma expanders on rats subjected to severe acute normovolemic hemodilution

作     者：Guo-Xing You Bing-Ting Li Zhen Wang Quan Wang Ying Wang Jing-Xiang Zhao Lian Zhao Hong Zhou Guo-Xing You;Bing-Ting Li;Zhen Wang;Quan Wang;Ying Wang;Jing-Xiang Zhao;Lian Zhao;Hong Zhou

作者机构：Institute of Health Service and Transfusion MedicineBeijing 100850China 

出 版 物：《Military Medical Research》 (军事医学研究（英文版）)

年 卷 期：2021年第8卷第2期

页      面：183-195页

核心收录：

学科分类：1002[医学-临床医学] 1010[医学-医学技术（可授医学、理学学位）] 100215[医学-康复医学与理疗学] 10[医学] 

基　　金：supported by the National Key Research and Development Program of China(2018YFC1106503) 

主　　题：Acute normovolemic hemodilution Plasma expander Acid-base Lactate Blood gas Physiological arameters 

摘      要：Background:Plasma expanders are widely used for acute normovolemic hemodilution(ANH).However,existing studies have not focused on large-volume infusion with colloidal plasma expanders,and there is a lack of studies that compare the effects of different plasma ***:The viscosity,hydrodynamic radius(Rh)and colloid osmotic pressure(COP)of plasma expanders were determined by a cone-plate viscometer,Zetasizer and cut-off membrane,*** male rats were randomized into five groups with Gelofusine(Gel),Hydroxyethyl Starch 200/0.5(HES200),Hydroxyethyl Starch 130/0.4(HES130),Hydroxyethyl Starch 40(HES40),and Dextran 40(Dex40),with 12 rats used in each group to build the ANH *** was performed by the withdrawal of blood and simultaneous infusion of plasma ***-base,lactate,blood gas and physiological parameters were ***:Gel had a lower intrinsic viscosity than HES200 and HES130(P0.01),but at a low shear rate in a mixture of colloids,red cells and plasma,Gel had a higher viscosity(P0.05 or P0.01,respectively).For hydroxyethyl starch plasma expanders,the COP at a certain concentration decreases from 11.1 mmHg to 6.1 mmHg with the increase of Rh from 10.7 nm to 20.2 nm.A severe ANH model,with the hematocrit of 40%of the baseline level,was established and accompanied by disturbances in acid-base,lactate and blood gas *** the end of ANH and 60 min afterward,the Dex40 group showed a worse outcome in maintaining the acid-base balance and systemic oxygenation compared to the other *** systolic blood pressure(SBP),diastolic blood pressure(DBP),and mean arterial pressure(MAP)decreased significantly in all groups at the end of *** DBP and MAP in the Dex40 group further decreased 60 min after the end of *** the process of ANH,the Dex40 group showed a drop and recovery in SBP,DBP and *** DBP and MAP in the HES200 group were significantly higher than those in the other groups at some time points(P0.05 o