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Occurrence of viable but non-culturable(VBNC)pathogenic bacteria in tap water of public places

Frontiers of Environmental Science & Engineering 2024年第3期18卷 115-129页

作者： Lizheng Guo Xinyan Xiao Kassim Chabi Yiting Zhang Jingjing Li Su Yao Xin Yu China National Research Institute of Food and Fermentation Industries Co. Ltd.China Center of Industrial Culture CollectionBeijing 100015China Key Laboratory of Urban Environment and Health Institute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China College of Environment&Ecology Xiamen UniversityXiamen 361102China

Viable but non-culturable(VBNC)bacteria have been detected in source water and effluent of drinking water treatment processes,leading to significant underestimation of viable cell *** information exists on VBNC bacteria in tap water,particularly in public *** address this gap,a comprehensive nine-month study was conducted in a major city in south-eastern China,using culture-based and quantitative PCR with propidium monoazide(PMA)dye ***-five samples were collected from five representative public places(railway station,campus,hospital,shopping mall,and institution).The findings revealed that culturable bacteria represented only 0–17.51%of the viable 16S rRNA genes,suggesting that the majority of viable bacteria existed in an uncultured or VBNC ***,opportunistic pathogens such as Escherichia coli,Enterococcus faecalis,Pseudomonas aeruginosa,Salmonella sp.,and Shigella *** primarily detected as VBNC cells,with concentrations ranging from 1.03×10^(0)to 3.01×10^(3),1.20×10^(0)to 1.42×10^(2),1.32×10^(0)to 8.82×10^(0),1.00×10^(0)to 6.71×10^(1),and 2.07×10^(0)to 1.93×10^(2)cell equivalent/100 mL,*** *** was observed in tap water after prolonged stagnation,indicating potential risks associated with bacterial *** and temporal factors accounted for 17.1%and 26.0%,respectively,of the variation in tap water community structure during the sampling period,as revealed by 16S rRNA amplicon *** study provides quantitative insights into the occurrence of VBNC bacteria in tap water and highlights the need for more sensitive monitoring methods and microbial control techniques to enhance tap water safety in public locations.

关键词： VBNC pathogenic bacteria PMA treatment Public tap water Community structure

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Influencing factors on the preservation of lytic bacteriophage VP3

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Biosafety and Health 2022年第5期4卷 314-320页

作者： Yue Xiao Pin Huang Zhenzhou Huang Keyi Yu Yang Song Ning Guo Hang Dai Mengnan Jiang Yi Xu Duochun Wang Qiang Wei center for human pathogenic culture collection National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Institute of Biothermal Science&Technology the University of Shanghai for Science and TechnologyShanghai 200093China National Pathogen Resource center Chinese Center for Disease Control and PreventionBeijing 102206China

Long-term and stable preservation of bacteriophages is of crucial *** many efforts have been made in the past decades to explore the influence of external factors on bacteriophage preservation,there is still little understanding,and a systematic description is *** this study,we explored the influence of different factors on the preservation of lytic bacteriophage VP3,one of the typing bacteriophages of Vibrio cholerae O1 biotype El Tor,and attempted to optimize its *** examined external factors,including temperature,solution,and cryoprotectant,in stable cooling/freezing conditions or alternate cooling/freezing and *** found that whether in Luria-Bertani(LB)medium or SM buffer,in terms of 20-week stable cooling or freezing,−20℃ was the most damaging while 4℃,−80℃,and−196℃ were *** cycles of alternate cooling/freezing and thawing caused a loss in the survival rates of *** addition of cryoprotectant,glycerol(30%,w/v)or dimethyl sulfoxide(DMSO,10%,w/v)significantly improved the survival rates of bacteriophages preserved at−20℃.However,at 4℃,−80℃,and−196℃,the cryoprotectant effect was only slightly positive or even *** summary,for bacteriophage VP3,the best preservation method is to directly preserve the bacteriophage stocks in LB medium at−80℃ or−196℃ instead of storing them in SM buffer or adding *** results provided insights into the external influencing factors on bacteriophage VP3 during preservation at low temperature and can be applied to the optimization of bacteriophage preservation in the future.

关键词： Influencing factors Preservation Lytic bacteriophage Cryoprotectant

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HIV complicated with Rhodococcus equi infection:A case report

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Infectious Medicine 2022年第4期1卷 281-284页

作者： Xinmin Xu Hongyuan Liang Yang Song Duochun Wang Qiang Wei Yajie Wang Department of Clinical Laboratory Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China The National Clinical Department of Infectious Diseases Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China National Pathogen Resource center Chinese Center for Disease Control and Prevention(China CDC)Beijing 102206China center for human pathogenic culture collection National Institute for Communicable Disease Control and PreventionChina CDCBeijing 102206China

Rhodococcus equi is a zoonotic opportunistic pathogen that mainly infects immunodeficient individuals,such as those with HIV *** ***-infected individuals,serious lung lesions can develop and death may result without appropriate antiviral *** bacterium is rare in clinic and there is little information regarding its diagnosis and *** improve our understanding,this case report describes the diagnosis and treatment of a patient with HIV complicated with *** infection from Ditan Hospital,Beijing,China.

关键词： Rhodococcus equi Pulmonary infection Case report

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Advances in technology for the laboratory diagnosis of individuals with HIV/AIDS coinfected with Mycobacterium tuberculosis

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Biosafety and Health 2024年第3期6卷 133-142页

作者： Jin Sun Xiaoxu Han Hongxia Yan Xin Zhang Taiyi Jiang Tong Zhang Hao Wu Grigory Kaminskiy Yingmin Ma Eduard Karamov Bin Su Beijing Key Laboratory for HIV/AIDS Research Clinical and Research Center for Infectious DiseasesBeijing Youan HospitalCapital Medical UniversityBeijing 100069China State Health Organization Tula Regional center for Control and Prevention of AIDS and Infectious Diseases Tula 300002Russia Department of Respiratory and Critical Care Medicine Beijing Youan HospitalCapital Medical UniversityBeijing 100069China Gamaleya National Research Centre for Epidemiology and Microbiology of the Ministry of Health of the Russian Federation Moscow 123098Russia

The high morbidity and mortality rate of individuals with human immunodeficiency virus(HIV)/acquired immunodeficiency syndrome(AIDS)coinfected with Mycobacterium tuberculosis(MTB)is a tough challenge for current global tuberculosis prevention and control ***/MTB coinfection is more complex than a single infection,and the interaction between the two diseases aggravates the deterioration caused by the disease,resulting in increased hospitalizations and *** screening and early diagnosis facilitate the timely initiation of anti-tuberculosis treatment in HIV/MTB coinfected individuals,thereby reducing transmission and the incidence of adverse *** date,pathogenic detection has remained the gold standard for diagnosing tuberculosis,but its sensitivity and specificity are greatly affected by the body's immune status,which limits its application in the diagnosis of HIV/MTB ***,immunology and molecular detection technology has developed *** detection technologies,such as interferon-γ release assays,interferon-gamma inducible protein 10,and GeneXpert MTB/RIF assay have overcome the limitations of traditional detection methods,significantly improved the sensitivity and specificity of tuberculosis diagnosis,and brought new hope to the detection of HIV/MTB *** this article,the principle,scope of application,and latest research progress of relevant detection methods are reviewed to provide a reference for the early diagnosis of HIV/MTB coinfection.

关键词： human immunodeficiency virus(HIV) Mycobacterium tuberculosis(MTB)coinfection Mycobacterium tuberculosis(MTB) Early diagnosis

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HCV NS5A and NS5B Enhance Expression of human Ceramide Glucosyltransferase Gene

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Virologica Sinica 2012年第1期27卷 38-47页

作者： Jia Guo Ran Yan Guo-dong Xu Cong-yi Zheng State Key Laboratory of Virology College of Life SciencesWuhan UniversityWuhan 430072China China center for Type culture collection Wuhan UniversityWuhan 430072China

Host genes involved in lipid metabolism are differentially affected during the early stages of hepatitis C virus (HCV) infection. Here we demonstrate that artificial up-regulation of fatty acid biosynthesis has a positive effect on the replication of the HCV full-length replicon when cells were treated with nystatin. Conversely, the HCV RNA replication was decreased when fatty acid biosynthesis was inhibited with 25-hydroxycholesterol and PDMP(D-threo-1-phenyl-2-decanoylamino-3- morpholino-1-propanol). In agreement with these results, the expression level of GlcT-1(ceramide glucosyltransferase), a host glucosyltransferase in the first step of GSL (glycosphingolipid) biosynthesis, was found to be closely associated with the expression and replication of HCV RNA. On the other hand, the viral RNA can also activate GlcT-1 in the early stage of viral RNA transfection in vitro. To identify viral factors that are responsible for GlcT-1 activation, we constructed ten stable Vero cell lines that express individual HCV proteins. Based on the analyses of these cell lines and transient transfection assay of the GlcT-1 promoter regions, we conclude that HCV proteins, especially NS5A and NS5B, have positive effects on the expression of GlcT-1. It is possible that NS5A and NS5B stimulate transcription factor(s) to activate the expression of GlcT-1 by increasing its transcription level.

关键词：丙型肝炎病毒神经酰胺葡萄糖基因表达脂肪酸合成人力马尔可夫过程早期阶段

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A broadly neutralizing human monoclonal antibody against the hemagglutinin of avian influenza virus H7N9

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Chinese Medical Journal 2022年第7期135卷 799-805页

作者： Jingxin Li Li Zhang Linlin Bao Yuxiao Wang Lin Qiu Jialei Hu Rong Tang Huiyan Yu Jun Shan Yan Li Chuan Qin Fengcai Zhu Department for Vaccine Clinical Evaluation NHC Key Laboratory of Enteric Pathogenic MicrobiologyJlangsu Provincial Center for Diseases Control and PreventionNanjingJiangsu 210009China Institute of Laboratory Animal Science Chinese Academy of Medical Sciences(CAMS)&Comparative Medicine CenterPeking Union Medical Collage(PUMC)Key Laboratory of Human Disease Comparative MedicineMinistry of HealthBeijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious DiseasesBeijing 100101China Department of Public Health Southeast UniversityNanjingJiangsu 210009China Shanghai Institute for Biological Sciences Chinese Academy of SciencesShanghai 200031China

Background:The new emerging avian influenza A H7N9 virus,causing severe human infection with a mortality rate of around 41%.This study aims to provide a novel treatment option for the prevention and control of ***:H7 hemagglutinin(HA)-specific B cells were isolated from peripheral blood plasma cells of the patients previously infected by H7N9 in Jiangsu Province,*** human monoclonal antibodies(mAbs)were generated by amplification and cloning of these HA-specific B ***,all human mAbs were screened for binding activity by enzyme-linked immunosorbent ***,those mAbs,exhibiting potent affinity to recognize H7 HAs were further evaluated by hemagglutination-inhibiting(HAI)and microneutralizationin vitro ***,the lead mAb candidate was selected and tested against the lethal challenge of the H7N9 virus using murine ***:The mAb 6-137 was able to recognize a panel of H7 HAs with high affinity but not HA of other subtypes,including H1N1 and *** mAb 6-137 can efficiently inhibit the HA activity in the inactivated H7N9 virus and neutralize 100 tissue culture infectious dose 50(TCID_(50))of H7N9 virus(influenza A/Nanjing/1/2013)invitro,with neutralizing activity as low as 78 ng/*** addition,the mAb 6-137 protected the mice against the lethal challenge of H7N9 prophylactically and ***:The mAb 6-137 could be an effective antibody as a prophylactic or therapeutic biological treatment for the H7N9 exposure or infection.

关键词： Avian influenza H7N9 Monoclonal antibody Neutralizing activity

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The monkey microbial biobank brings previously uncultivated bioresources for nonhuman primate and human gut microbiomes

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mLife 2022年第2期1卷 210-217页

作者： Danhua Li Chang Liu Rexiding Abuduaini Mengxuan Du Yujing Wang Haizhen Zhu Honghe Chen Nan Zhou Yuhua Xin Linhuan Wu Juncai Ma Yuguang Zhou Yong Lu Chengying Jiang Qiang Sun Shuang-Jiang Liu State Key Laboratory of Microbial Resources and Environmental Microbiology Research center at Institute of Microbiology Chinese Academy of SciencesBeijingChina College of Life Science University of Chinese Academy of SciencesBeijingChina State Key Laboratory of Microbial Biotechnology Shandong UniversityQingdaoChina China General Microorganism culture collection Institute of MicrobiologyChinese Academy of SciencesBeijingChina Microbial Resources and Big Data center Institute of MicrobiologyChinese Academy of SciencesBeijingChina State Key Laboratory of Neuroscience CAS Key Laboratory of Primate NeurobiologyCAS Center for Excellence in Brain Science and Intelligence TechnologyChinese Academy of SciencesInstitute of NeuroscienceShanghaiChina

Impact statement Nonhuman primates(NHPs)such as monkeys are the closest living relatives to humans and are the best available models for causative studies of human health and *** microbiomes are intensively involved in host *** this study,by large-scale cultivation of microbes from fecal samples of monkeys,we obtained previously uncultured bacterial species and constructed a Macaca fascicularis Gut Microbial Biobank(MfGMB).The MfGMB consisted of 250 strains that represent 97 species of 63 genera,25 families,and 4 *** information of the 250 strains and the genomes of 97 cultured species are publicly *** MfGMB represented nearly 50% of core gut microbial compositions at the genus level and covered over 80% of the KO-based known gut microbiome functions of *** mining showed that the bacterial species in the MfGMB were prevalent not only in NHPs gut microbiomes but also in human gut *** study will help the understanding and future investigations on how gut microbiomes interact with their mammalian hosts.

关键词： statement monkey gut

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Detection and evaluation of different morphological forms of Mycoplasma hominis in human semen

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Asian Journal of Andrology 2024年第1期26卷 71-76页

作者： Elizaveta Bragina Tatyana Sorokina Avtandil Chogovadze Yulia Shevchenko Vyacheslav Chernykh Olga Barkhatova Irina Rakovskaya Research Centre for Medical Genetics Moscow 115522Russia A.N.Belozersky Institute of Physico-Chemical Biology Lomonosov Moscow State University Moscow 119992Russia Reprolab Limited Liability Company Moscow 119333Russia N.I.Pirogov Russian National Research Medical University Moscow 117997Russia Federal Government Budgetary Institution"National Research center for Epidemiology and Microbiology Named after Honorary Academician N.F.Gamaleya of the Ministry of Health of the Russian Federation" Moscow 123098Russia

Recently discovered microcolonial forms of Mycoplasma hominis(***)and their impact on human spermatogenesis are *** spermatozoa of 125 fertile men(sperm donors;from Reprobank[Reproductive Tissue Bank,Moscow,Russial)and of 93 patients with fertility problems(from the Federal State Budgetary Institution"Research Centre for Medical Genetics[RCMG]",Moscow,Russia)were *** colonies of *** and microcolonies were detected by molecular biological methods,culture of bacteria,and transmission electron *** unique structure of microcolonial cells,in which the cytoplasmic cylinder is surrounded by concentric electron-dense and electron-light layers with a periodicity of 12-14 nm,and the ability of microcolonial cells to attach to spermatozoa are *** patients with lower sperm quality,microcolonies of *** were detected 2.5 times more frequently than classical *** detection of microcolonies in the ejaculate and the frequent isolation of microcolonies from sperm samples of patients with fertility problems suggest that microcolonial cells may be one cause of infertility.

关键词： ejaculate fertility Mycoplasma hominis spermatogenesis transmission electron microscopy

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Transcriptomic analyses reveal new genes and networks response to H5N1 influenza viruses in duck(Anas platyrhynchos)

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Journal of Integrative Agriculture 2019年第7期18卷 1460-1472页

作者： HUANG Yin-hua FENG Hua-peng HUANG Li-ren YI Kang RONG En-guang CHEN Xiao-yun LI Jian-wen WANG Zeng ZHU Peng-yang LIU Xiao-juan WANG Xiao-xue HU Jia-xiang LIU Xin CHEN Hua-lan WANG Jun LI Ning State Key Laboratory for Agrobiotechnology China Agricultural UniversityBeijing 100193P.R. China State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China BGI-Shenzhen Shenzhen 518083P.R.China China Veterinary culture collection center China Institute of Veterinary Drug ControlBeijing 100081P.R.China

H5N1 influenza represents one of the great challenges to public *** H5N1 viruses(i.e.,A/goose/Hubei/65/05,GS/65) are weakly pathogenic,while the others(i.e.,A/duck/Hubei/49/05,DK/49) are highly pathogenic to their natural ***,we performed brain and spleen transcriptomic analyses of control ducks and ones infected by the DK/49 or the GS/65 H5N1 *** demonstrated that,compared to the GS/65 virus,the DK/49 virus infection changed more numerous immune genes’ expression and caused continuous increasing of immune pathways(i.e.,RIG-I and MDA5) in *** found that both H5N1 virus strains might escape or subvert host immune response through affecting alternative translation of immune genes,while the DK/49 virus seemed to induce alternative translation of more immune genes than the GS/65 *** also identified five co-expressional modules associated with H5N1 virus replication through the weight correlation network analysis(WGCNA).Moreover,we first demonstrated that the duck BCL2 L15 and DCSTAMP in one of these five modules inhibited both the highly pathogenic and weakly pathogenic H5N1 virus replication *** analyses,in combination with our comprehensive transcriptomic data,provided global view of the molecular architecture for the interaction between host and H5N1 viruses.

关键词： duck innate immune genes H5N1 influenza viruses transcriptomes

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An Effective Platform for Exploring Rotavirus Receptors by Bacterial Surface Display System

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Virologica Sinica 2020年第1期35卷 103-109页

作者： Danlei Liu Haoran Geng Zilei Zhang Yifan Xing Danlu Yang Zhicheng Liu Dapeng Wang Department of Food Science and Technology School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China State Key Laboratory of Applied Microbiology Southern China Guangdong Provincial Key Laboratory of Microbial Culture Collection and ApplicationGuangdong Open Laboratory of Applied MicrobiologyGuangdong Institute of MicrobiologyGuangzhou 510070China Shanghai Food Safety and Engineering Technology Research center School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China

Rotavirus(RV) is a major foodborne pathogen. For RV prevention and control, it is a key to uncover the interaction mechanism between virus and its receptors. However, it is hard to specially purify the viral receptors, including histo-blood group antigens(HBGAs). Previously, the protruding domain protein(P protein) of human norovirus(genotype Ⅱ.4) was displayed on the surface of Escherichia coli, and it specifically recognized and captured the viral ligands. In order to further verify the feasibility of the system, P protein was replaced by VP8* of RV(G9 P[8]) in this study. In the system, VP8*could be correctly released by thrombin treatment with antigenicity retaining, which was confirmed by Western blot and Enzyme-Linked Immunosorbent Assays. Type A HBGAs from porcine gastric mucin(PGM) were recognized and captured by this system. From saliva mixture, the captured viral receptor bound with displayed VP8* was confirmed positive with monoclonal antibody against type A HBGAs. It indicated that the target ligands could be easily separated from the complex matrix. These results demonstrate that the bacterial surface display system will be an effective platform to explore viral receptors/ligands from cell lines or food matrix.

关键词： Rotavirus Bacterial surface display system Receptors Histo-blood group antigens(HBGAs) Saliva

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