An Effective Platform for Exploring Rotavirus Receptors by Bacterial Surface Display System

作     者：Danlei Liu Haoran Geng Zilei Zhang Yifan Xing Danlu Yang Zhicheng Liu Dapeng Wang Danlei Liu;Haoran Geng;Zilei Zhang;Yifan Xing;Danlu Yang;Zhicheng Liu;Dapeng Wang

作者机构：Department of Food Science and TechnologySchool of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China State Key Laboratory of Applied Microbiology Southern ChinaGuangdong Provincial Key Laboratory of Microbial Culture Collection and ApplicationGuangdong Open Laboratory of Applied MicrobiologyGuangdong Institute of MicrobiologyGuangzhou 510070China Shanghai Food Safety and Engineering Technology Research CenterSchool of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2020年第35卷第1期

页      面：103-109页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：the National Key Research and Development Program of China(2017YFF0210200) the National Natural Science Foundation of China(31772078) 

主　　题：Rotavirus Bacterial surface display system Receptors Histo-blood group antigens(HBGAs) Saliva 

摘      要：Rotavirus(RV) is a major foodborne pathogen. For RV prevention and control, it is a key to uncover the interaction mechanism between virus and its receptors. However, it is hard to specially purify the viral receptors, including histo-blood group antigens(HBGAs). Previously, the protruding domain protein(P protein) of human norovirus(genotype Ⅱ.4) was displayed on the surface of Escherichia coli, and it specifically recognized and captured the viral ligands. In order to further verify the feasibility of the system, P protein was replaced by VP8* of RV(G9 P[8]) in this study. In the system, VP8*could be correctly released by thrombin treatment with antigenicity retaining, which was confirmed by Western blot and Enzyme-Linked Immunosorbent Assays. Type A HBGAs from porcine gastric mucin(PGM) were recognized and captured by this system. From saliva mixture, the captured viral receptor bound with displayed VP8* was confirmed positive with monoclonal antibody against type A HBGAs. It indicated that the target ligands could be easily separated from the complex matrix. These results demonstrate that the bacterial surface display system will be an effective platform to explore viral receptors/ligands from cell lines or food matrix.