Occurrence of viable but non-culturable(VBNC)pathogenic bacteria in tap water of public places
作者机构：China National Research Institute of Food and Fermentation Industries Co.Ltd.China Center of Industrial Culture CollectionBeijing 100015China Key Laboratory of Urban Environment and HealthInstitute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China College of Environment&EcologyXiamen UniversityXiamen 361102China
出 版 物：《Frontiers of Environmental Science & Engineering》 (环境科学与工程前沿（英文）)
年 卷 期：2024年第18卷第3期
页 面：115-129页
核心收录：
学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 10[医学]
基 金：supported by the National Natural Science Foundation of China(Nos.41861144023 and U2005206) the Xiamen Municipal Bureau of Science and Technology(No.YDZX20203502000003)
主 题：VBNC Pathogenic bacteria PMA treatment Public tap water Community structure
摘 要：Viable but non-culturable(VBNC)bacteria have been detected in source water and effluent of drinking water treatment processes,leading to significant underestimation of viable cell *** information exists on VBNC bacteria in tap water,particularly in public *** address this gap,a comprehensive nine-month study was conducted in a major city in south-eastern China,using culture-based and quantitative PCR with propidium monoazide(PMA)dye ***-five samples were collected from five representative public places(railway station,campus,hospital,shopping mall,and institution).The findings revealed that culturable bacteria represented only 0–17.51%of the viable 16S rRNA genes,suggesting that the majority of viable bacteria existed in an uncultured or VBNC ***,opportunistic pathogens such as Escherichia coli,Enterococcus faecalis,Pseudomonas aeruginosa,Salmonella sp.,and Shigella *** primarily detected as VBNC cells,with concentrations ranging from 1.03×10^(0)to 3.01×10^(3),1.20×10^(0)to 1.42×10^(2),1.32×10^(0)to 8.82×10^(0),1.00×10^(0)to 6.71×10^(1),and 2.07×10^(0)to 1.93×10^(2)cell equivalent/100 mL,*** *** was observed in tap water after prolonged stagnation,indicating potential risks associated with bacterial *** and temporal factors accounted for 17.1%and 26.0%,respectively,of the variation in tap water community structure during the sampling period,as revealed by 16S rRNA amplicon *** study provides quantitative insights into the occurrence of VBNC bacteria in tap water and highlights the need for more sensitive monitoring methods and microbial control techniques to enhance tap water safety in public locations.
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