Detection and evaluation of different morphological forms of Mycoplasma hominis in human semen

作     者：Elizaveta Bragina Tatyana Sorokina Avtandil Chogovadze Yulia Shevchenko Vyacheslav Chernykh Olga Barkhatova Irina Rakovskaya Elizaveta Bragina;Tatyana Sorokina;Avtandil Chogovadze;Yulia Shevchenko;Vyacheslav Chernykh;Olga Barkhatova;Irina Rakovskaya

作者机构：Research Centre for Medical GeneticsMoscow 115522Russia A.N.Belozersky Institute of Physico-Chemical Biology Lomonosov Moscow State UniversityMoscow 119992Russia Reprolab Limited Liability CompanyMoscow 119333Russia N.I.Pirogov Russian National Research Medical UniversityMoscow 117997Russia Federal Government Budgetary Institution"National Research Center for Epidemiology and Microbiology Named after Honorary Academician N.F.Gamaleya of the Ministry of Health of the Russian Federation"Moscow 123098Russia 

出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)

年 卷 期：2024年第26卷第1期

页      面：71-76页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：Ultrastructural research supported by the Moscow State University Development Program(PNR 5.13) The present study was performed within the framework of the project Multicenter Research Bioresource Collection Human Reproductive Health,No.15.BRK.21.0008 of the Ministry of Science and Higher Education of the Russian Federation 

主　　题：ejaculate fertility Mycoplasma hominis spermatogenesis transmission electron microscopy 

摘      要：Recently discovered microcolonial forms of Mycoplasma hominis(***)and their impact on human spermatogenesis are *** spermatozoa of 125 fertile men(sperm donors;from Reprobank[Reproductive Tissue Bank,Moscow,Russial)and of 93 patients with fertility problems(from the Federal State Budgetary InstitutionResearch Centre for Medical Genetics[RCMG],Moscow,Russia)were *** colonies of *** and microcolonies were detected by molecular biological methods,culture of bacteria,and transmission electron *** unique structure of microcolonial cells,in which the cytoplasmic cylinder is surrounded by concentric electron-dense and electron-light layers with a periodicity of 12-14 nm,and the ability of microcolonial cells to attach to spermatozoa are *** patients with lower sperm quality,microcolonies of *** were detected 2.5 times more frequently than classical *** detection of microcolonies in the ejaculate and the frequent isolation of microcolonies from sperm samples of patients with fertility problems suggest that microcolonial cells may be one cause of infertility.