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Characterization of human αβTCR repertoire and discovery of D-D fusion in TCRβ chains

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Protein & Cell 2014年第8期5卷 603-615页

作者： Peipei Liu Di Liu Xi Yang Jing Gaoai Ma3, Fangqing Zhaos, Xuyu Zhou~'2~, George F. Gao1'2'4'5~, Baoli Zhu~'2~ Yan Chen Xue Xiao Fei Liu Jing Zou Jun Wu Juncai Ma Fangqing Zhao Xuyu Zhou George F. Gao Baoli Zhu CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China Network Information Center Institute of Microbioiogy Chinese Academy of Sciences Beijing 100101 China Chinese Center for Disease Control and Prevention (China cdc) Beijing 102206 China Research Network of Immunity and Health (RNIH) Beijing Institutes of Life Science Chinese Academy of SciencesBeijing 100101 China

The characterization of the human T-cell receptor （TCR） repertoire has made remarkable progress, with most of the work focusing on the TCRβ chains. Here, we ana- lyzed the diversity and complexity of both the TCRa and TCRβ repertoires of three healthy donors. We found that the diversity of the TCRα repertoire is higher than that of the TCRβ repertoire, whereas the usages of the V and J genes tended to be preferential with similar TRAV and TRAJ patterns in all three donors. The V-J pairings, like the V and J gene usages, were slightly preferential. We also found that the TRDV1 gene rearranges with the majority of TRAJ genes, suggesting that TRDV1 is a shared TRAV/DV gene （TRAV42/DV1）. Moreover, we uncovered the presence of tandem TRBD （TRB D gene） usage in -2% of the productive human TCRβ CDR3 sequences.

关键词： TCR repertoire next-generationsequencing V/J usage V-J pairing CDR3 D-D fusion

Peroxidase-like active Cu-ZIF-8 with rich copper(I)-nitrogen sites for excellent antibacterial performances toward drug-resistant bacteria

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Nano Research 2024年第8期17卷 7427-7435页

作者： Xiaoze Wang Hui Wang Jingkun Zhang Ning Han Wenjun Ma Donghai Zhang Mingshui Yao Xianliang Wang Yunfa Chen State Key Laboratory of Mesoscience and Engineering Institute of Process EngineeringChinese Academy of SciencesBeijing 100190China University of the Chinese Academy of Sciences Beijing 100049China Laboratory of Theoretical and Computational Nanoscience CAS Key Laboratory of Nanosystem and Hierarchial FabricationCAS Center for Excellence in NanoscienceNational Center for Nanoscience and TechnologyBeijing 100190China China cdc Key Laboratory of Environment and Population Health National Institute of Environmental HealthChinese Center for Disease Control and PreventionBeijing 100021China

Bacterial pathogens pose a serious threat to human health,and there is an urgent need to develop highly effective antibacterial materials to eliminate the increasingly serious contamination of drug-resistant ***,a Cu-doped ZIF-8 particle with unsaturated copper exhibits high peroxidase-like activity.99.998%antibacterial efficiency against *** can be achieved for 30 min at a low concentration of 50μg·mL^(−1),as well as complete sterilization against ***(up to 8 log).99.999%antibacterial efficiency against Methicillin-resistant Staphylococcus aureus can be achieved,performing orders of magnitude higher than *** mechanism shows that the unsaturated Cu-Nx sites are enzyme-like active centers,which could promote the consumption of bacteria reducing substances by H_(2)O_(2),and the generated*OH further aggravates bacterial oxidative stress and membrane *** importantly,the oxidation activity of adsorbed oxygen species on Cu-ZIF-8 is enhanced by charge transfer and structural changes between the ligand and copper center like natural ***-doped ZIF-8 with peroxidase-like activity shows great potential in antibacterial application and the revealed catalytic mechanism is helpful for understanding the high antibacterial activity of nanoparticles with Cu-Nx sites.

关键词： Cu-doped ZIF-8 peroxidase-like reactive oxygen species(ROS) catalytic mechanism antibacterial activity

Structural Basis of Glycan Recognition in Globally Predominant Human P[8]Rotavirus

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Virologica Sinica 2020年第2期35卷 156-170页

作者： Xiaoman Sun Lei Dang Dandi Li Jianxun Qi Mengxuan Wang Wengang Chai Qing Zhang Hong Wang Ruixia Bai Ming Tan Zhaojun Duan National Health Commission Key Laboratory for Medical Virology and Viral Diseases Beijing 102206China National Institute for Viral Disease Control and Prevention China CDC.Beijing 102206China Inner Mongolia Medical University Huhehaote 010059China Institute of Microbiology Chinese Academy of SciencesBeijing 100101China Glycosciences Laboratory Department of MedicineImperial College LondonLondonUK Division of Infectious Diseases University of Cincinnati College of MedicineCincinnatiOHUSA

Rotavirus(RV)causes acute gastroenteritis in infants and children *** studies showed that glycans such as histo-blood group antigens(HBGAs)function as cell attachment factors affecting RV host susceptibility and prevalence.P[8]is the predominant RV genotype in humans,but the structural basis of how P[8]RVs interact with glycan ligands remains *** this study,we characterized the interactions between P[8]VP8~*s and glycans which showed that VP8~*,the RV glycan binding domain,recognized both mucin core 2 and H type 1 antigens according to the ELISA-based oligosaccharide binding ***,we determined the structural basis of P[8]RV-glycans interaction from the crystal structures of a Rotateq P[8]VP8~*in complex with core 2 and H type 1 glycans at 1.82.3?,respectively,revealing a common binding pocket and similar binding *** and sequence analysis demonstrated that the glycan binding site is conserved among RVs in the P[Ⅱ]genogroup,while genotype-specific amino acid variations determined different glycan binding *** data elucidated the detailed structural basis of the interactions between human P[8]RVs and different host glycan factors,shedding light on RV infection,epidemiology,and development of anti-viral agents.

关键词： Rotavirus(RV)·P Glycan binding specificity VPS*structure Mucin core 2 Lacto-N-fucopentaose 1(LNFPl)

Origin and molecular characterization of the human-infecting H6N1 infl uenza virus in Taiwan

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Protein & Cell 2013年第11期4卷 846-853页

作者： Weifeng Shi Yi Shi Ying Wu Di Liu George F.Gao School of Medical Sciences Taishan Medical CollegeTaian 271016China CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Beijing Institutes of Life Sciences Chinese Academy of SciencesBeijing 100101China Network Information Center Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Offi ce of Director-General Chinese Center for Disease Control and Prevention(China CDC)Beijing 102206China

In June 2013,the fi rst human H6N1 infl uenza virus infec-tion was confirmed in ***,the origin and molecular characterization of this virus,A/Taiwan/2/2013(H6N1),have not been well studied thus *** the present report,we performed phylogenetic and coalescent analy-ses of this virus and compared its molecular profi le/char-acteristics with other closely related *** characterization of H6N1 revealed that it is a typical avian infl uenza virus of low pathogenicity,which might not rep-licate and propagate well in the upper airway in *** analysis revealed that the virus clusters with A/chicken/Taiwan/A2837/2013(H6N1)in seven genes,except *** the PB1 gene,A/Taiwan/2/2013 was clus-tered with a different H6N1 lineage from A/chicken/Taiwan/A2837/*** a previous study demonstrated that the PB2,PA,and M genes of A/Taiwan/2/2013 might be derived from the H5N2 viruses,coalescent analyses revealed that these H5N2 viruses were derived from more recent strains than that of the ancestor of A/Taiwan/2/***,we propose that A/Taiwan/2/2013 is a reassor-tant from different H6N1 lineages circulating in chickens in ***,compared to avian isolates,a sin-gle P186L(H3 numbering)substitution in the hemaggluti-nin H6 of the human isolate might increase the mammali-an receptor binding and,hence,this strain’s pathogenicity in ***,human infection with this virus seems an accidental event and is unlikely to cause an infl uenza ***,its co-circulation and potential reas-sortment with other infl uenza subtypes are still worthy of attention.

关键词： molecular characterization phylogenetic analysis coalescent analysis H6N1 infl uenza virus Taiwan

Double-endpoint Genotoxicity Quantification and PAHs Characterization of Drinking Water Source alongside Polluted Yinghe River with High Tumor Mortality

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Current Medical Science 2021年第2期41卷 189-198页

作者： Wei ZHANG Chen GUO Xiao-li WANG Zhan-lu LV Lin FAN Yu-yan YANG Xu LI Jing QI Shu-li ZHAO Xian-liang WANG China cdc Key Laboratory of Environment and Population Health National Institute of Environmental HealthChinese Center for Disease Control and PreventionBeijing 100021China China State Key Laboratory of Environmental Criteria and Risk Assessment Chinese Research Academy of Environmental SciencesBeijing 100012China College of Life Science and Bioengineering Beijing University of TechnologyBeijing 100124China China National Environmental Monitoring Center Beijing 100012China

The etiology for the high tumor mortality in heavy polluted Yinghe river basin is still unclear and polycyclic aromatic hydrocarbons(PAHs)belong to the priority pollutants in water based on the former surveillance *** order to explore the potential genotoxicants contributing to the double-endpoint genotoxicity of polluted drinking water source,12 groundwater and 3 surface water samples were collected from 3 villages and the nearby rivers alongside Yinghe river basin,respectively and their comprehensive genotoxicity was estimated with a bioassay group of sOS/umu test and micronucleus(MN)test(MNT).Some groundwater samples showed positive genotoxicity and all surface water samples were highly *** groundwater samples showed DNA genotoxic effct with the average 4-NQO equivalent concentration(TEQ_(4-NQO))of 0.067μg/L and 0.089μg/L in wet and dry season,*** average MN ratios of groundwater samples were 14.19‰ and 17.52‰ in wet and dry season,*** samples showed different genotoxic effect among 3 *** total PAHs concentrations in all water samples ranged from 8.98 to 25.17 ng/L with an average of 14.97±4.85 ng/***,CHR,BkF,BaP and DBA were the main carcinogenic PAHs contributing to the genotoxicity of water *** conclusion,carcinogenic PAHs are possibly related to the high tumor mortality in the target *** of carcinogenic PAHs to genotoxicity of drinking water source may shed light on the etiology study for high tumor mortality in Yinghe river *** words:genotoxicity test;drinking water source;high tumor mortality;Yinghe river basin;polycyclic aromatic hydrocarbons(PAHs)

关键词： genotoxicity test drinking water source high tumor mortality Yinghe river basin polycyclic aromatic hydrocarbons(PAHs)

Molecular Characteristics and Phylogenetic Analysis of N Gene of Human Derived Rabies Virus

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Biomedical and Environmental Sciences 2011年第4期24卷 431-437页

作者： CAI Liang TAO XiaoYan LIU YunZhi ZHANG Hong GAO LiDong HU ShiXiong LIU FuQiang LI Hao SHEN XinXin LIU JiaHui WANG ShiQing TANG Qing Hunan Center for Disease Control and Prevention Key Laboratory of Microbial Molecular Biology of Hunan Province Changsha 410005 Hunan China State Key Laboratory for Molecular Virology and Genetic Engineering Institute for Viral Disease Control and Prevention China CDC Beijing 102206 China

Objective To investigate the relationship between the molecular characteristics and phylogenetic evolution of rabies N *** Saliva samples were collected from rabies cases,and RT-PCR was used to amplify the N gene of rabies virus with the specific *** amplifying product of RT-PCR was cloned to pUCm-T vector and transformed into *** XL1-Blue and then the blue-white selection,PCR screening and gene sequencing were carried out to identify the positive ***,ExPASy and other bioinformatics softwares were used to analyze and predict the structure and biological characteristics of the N *** The amplification product of RT-PCR was 1 353 bp,the recombinant plasmid pUCm-T/N was constructed,the whole length of the N gene open reading frame was composed of 1 353 nucleotide residues to code 450 amino acids （20 kinds）,the accession number submitted to the Genbank was HM756692,its sequence homology of nucleotides and amino acids compared with the vaccine strain CTN-1-V was 90% and 99% *** evolutionary analysis showed that the isolated strain belonged to genotype I with certain geographic *** The characteristics investigation and bioinformatics analysis of Hunan0806 N gene will provide fundament data to reveal the significance of the N gene characteristics for rabies epidemiology and its prevention control.

关键词： Rabies N gene Molecular Characteristics Phylogenetic

Mechanisms of melatonin in anti-aging and its regulation effects in radiation-induced premature senescence

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Radiation Medicine and Protection 2021年第1期2卷 33-37页

作者： Liping Ma Qingjie Liu Mei Tian Xuelei Tian Ling Gao Key Laboratory of Radiation Protection and Nuclear Emergency China CDCNational Institute for Radiological ProtectionChinese Center for Disease Control and PreventionBeijing100088China

Melatonin is an effective antioxidant hormone produced mainly by the pineal *** senescence refers to senescence induced by declined cell proliferation and physiological functions when cells are stimulated by nontelomeric *** anti-aging effect of melatonin is exerted by upregulating the expression of silent information regulator 1/Sirtuin 1(SIRT1),which reduces oxidative stress damage,decreases p53 activation,and inhibits the NF-κB *** can induce premature senescence through direct or indirect oxidative stress *** mechanism by which melatonin regulates radiation-induced premature senescence includes the inhibition of the p53-mediated senescence pathway through elimination of reactive oxygen species or p53 deacetylation induced by upregulation of SIRT1 *** exhibits different regulatory effects for different cells and types of radiation.

关键词： Melatonin Premature senescence Anti-aging Radiation p53

Effects of Cotrimoxazole Prophylaxis Initiation and Discontinuation on Mortality and Attrition Rates among HIV Patients Who Initiate ART in Southwest China:An Observational Cohort Study

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Biomedical and Environmental Sciences 2021年第8期34卷 646-649页

作者： ZHU Jin Hui RUAN Yu Hua PAN Stephen YANG Wen Min ZHU Qiu Ying CHEN Huan Huan SHEN Zhi Yong LAN Guang Hua XING Hui SHAO Yi Ming LI Li Ming School of Public Health Peking UniversityBeijing 100191China Guangxi Center for Disease Control and Prevention Nanning 530028GuangxiChina State Key Laboratory of Infectious Disease Prevention and Control(SKLID) Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesChinese Center for Disease Control and Preventio n(China CDC)Beijing 100032China Department of Health and Environ mental Sciences Xi'an Jiaotong-Liverpool UniversitySuzhou 215123JiangsuChina

Combination antiretroviral therapy(ART)reduced AIDS-related mortality and increased survival among patients living with HIV by interrupting HIV replication,enhancing immune recovery,and preventing the onset of opportunistic infections[1].In China,ART has rapidly been scaled up since the beginning of the National Free Antiretroviral Treatment Program(NFATP)in 2003[2].By the end of 2016,489,411 individuals diagnosed with HIV were receiving free antiretroviral treatment in *** is firmly committed to reducing overall AIDS-related mortality and HIV incidence within the ***,similar to other low-and middle-income countries,the NFATP is challenged by high mortality and attrition shortly after patients initiate ART[3].

关键词： mortality Observation continuation

Achieving the Goals of Healthy China 2030 Depends on Increasing Smoking Cessation in China:Comparative Findings from the ITC Project in China,Japan,and the Republic of Korea

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China cdc weekly 2021年第22期3卷 463-467页

作者： Geoffrey T.Fong Jiang Yuan Lorraine V.Craig Steve Shaowei Xu Gang Meng Anne C.K.Quah Hong-Gwan Seo Sungkyu Lee Itsuro Yoshimi Kota Katanoda Takahiro Tabuchi University of Waterloo WaterlooOntarioCanada Ontario Institute for Cancer Research TorontoOntarioCanada Tobacco Control Office China CDCBeijingChina Korea National Cancer Center SeoulRepublic of Korea Korea Center for Tobacco Research and Education SeoulRepublic of Korea Japan National Cancer Center TokyoJapan Osaka International Cancer Institute OsakaJapan.

Summary Tobacco smoking is the number one preventable cause of disease and death in China as it is ***,the toll of smoking in China is much greater than its status as the world’s most populous *** is a persistent and continuing need for China to implement the measures specified in the global tobacco control treaty,the World Health Organization(WHO)Framework Convention on Tobacco Control(FCTC),which China ratified in 2005.

关键词： globally ITC specified