Molecular Characteristics and Phylogenetic Analysis of N Gene of Human Derived Rabies Virus

作     者：CAI Liang TAO XiaoYan LIU YunZhi ZHANG Hong GAO LiDong HU ShiXiong LIU FuQiang LI Hao SHEN XinXin LIU JiaHui WANG ShiQing TANG Qing 

作者机构：Hunan Center for Disease Control and Prevention Key Laboratory of Microbial Molecular Biology of Hunan Province Changsha 410005 Hunan China State Key Laboratory for Molecular Virology and Genetic Engineering Institute for Viral Disease Control and Prevention China CDC Beijing 102206 China 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2011年第24卷第4期

页      面：431-437页

核心收录：

学科分类：090601[农学-基础兽医学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 09[农学] 0906[农学-兽医学] 

基　　金：supported by the science foundation of the Health Department of Hunan Province (Grant No A2007008 and B2009-088) the National Department Public Benefit Research Foundation (Grant No 200803014) 

主　　题：Rabies N gene Molecular Characteristics Phylogenetic 

摘      要：Objective To investigate the relationship between the molecular characteristics and phylogenetic evolution of rabies N *** Saliva samples were collected from rabies cases,and RT-PCR was used to amplify the N gene of rabies virus with the specific *** amplifying product of RT-PCR was cloned to pUCm-T vector and transformed into *** XL1-Blue and then the blue-white selection,PCR screening and gene sequencing were carried out to identify the positive ***,ExPASy and other bioinformatics softwares were used to analyze and predict the structure and biological characteristics of the N *** The amplification product of RT-PCR was 1 353 bp,the recombinant plasmid pUCm-T/N was constructed,the whole length of the N gene open reading frame was composed of 1 353 nucleotide residues to code 450 amino acids （20 kinds）,the accession number submitted to the Genbank was HM756692,its sequence homology of nucleotides and amino acids compared with the vaccine strain CTN-1-V was 90% and 99% *** evolutionary analysis showed that the isolated strain belonged to genotype I with certain geographic *** The characteristics investigation and bioinformatics analysis of Hunan0806 N gene will provide fundament data to reveal the significance of the N gene characteristics for rabies epidemiology and its prevention control.