Characterization of human αβTCR repertoire and discovery of D-D fusion in TCRβ chains

作     者：Peipei Liu Di Liu Xi Yang Jing Gaoai Ma3, Fangqing Zhaos, Xuyu Zhou~'2~, George F. Gao1'2'4'5~, Baoli Zhu~'2~ Yan Chen Xue Xiao Fei Liu Jing Zou Jun Wu Juncai Ma Fangqing Zhao Xuyu Zhou George F. Gao Baoli Zhu 

作者机构：CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China Network Information Center Institute of Microbioiogy Chinese Academy of Sciences Beijing 100101 China Chinese Center for Disease Control and Prevention (China CDC) Beijing 102206 China Research Network of Immunity and Health (RNIH) Beijing Institutes of Life Science Chinese Academy of SciencesBeijing 100101 China 

出 版 物：《Protein & Cell》 (蛋白质与细胞（英文版）)

年 卷 期：2014年第5卷第8期

页      面：603-615页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 080802[工学-电力系统及其自动化] 0808[工学-电气工程] 08[工学] 0906[农学-兽医学] 09[农学] 0703[理学-化学] 0836[工学-生物工程] 

基　　金：We thank Dr. Christopher J. Vavrickafor and Boris Tefsen for their critical reading and revision of the manuscript and Dr. Miles P. Dav- enport for his inspiring discussions. This work is supported by the National Natural Science Foundation of China (NSFC  Grant No. 31030030)  the National Basic Research Program (973 Program) (No. 2013CB531500) and the National Natural Science Foundation of China (Grant No. 81373141 ). G.F.G. is a leading principal investigator of the NSFC Innovative Research Group (Grant No. 81321063) 

主　　题：TCR repertoire next-generationsequencing V/J usage V-J pairing CDR3 D-D fusion 

摘      要：The characterization of the human T-cell receptor （TCR） repertoire has made remarkable progress, with most of the work focusing on the TCRβ chains. Here, we ana- lyzed the diversity and complexity of both the TCRa and TCRβ repertoires of three healthy donors. We found that the diversity of the TCRα repertoire is higher than that of the TCRβ repertoire, whereas the usages of the V and J genes tended to be preferential with similar TRAV and TRAJ patterns in all three donors. The V-J pairings, like the V and J gene usages, were slightly preferential. We also found that the TRDV1 gene rearranges with the majority of TRAJ genes, suggesting that TRDV1 is a shared TRAV/DV gene （TRAV42/DV1）. Moreover, we uncovered the presence of tandem TRBD （TRB D gene） usage in -2% of the productive human TCRβ CDR3 sequences.