检索结果-南通市图书馆

One-step polymerized lanthanide-based polystyrene microsphere for sensitive lateral flow immunoassay

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Rare Earths 2021年第1期39卷 11-18,I0001页

作者： Zhenhua Li Qingyun Liu Yongfang Li Wei Yuan Fuyou Y.Li State Key Laboratory of Molecular Engineering of Polymers Department of ChemistryFudan UniversityShanghai200433China

Lateral flow immunoassays(LFIAs)provide a powerful tool for rapid real-time assay of cancer biomarkers,which is vital for cancer detection and treatment ***-based LFIAs is one of the most widely used methods,especially Eu(Ⅲ)chelates,which possess distinctive and attractive characteristics,such as time-resolved fluorescence and large Stokes ***,we adopted a new onestep mini-emulsion polymerization method to synthesize carboxyl-modified fluorescent microsphere(OS-EuCM),which shows good stability,resistance to non-specific adhesion and uniform particle size distribution compared with traditional microspheres synthesized through the swelling *** from the above advantages,OS-EuCM was successfully used in LFIAs to detect tumor marker Alpha-fetoprotein with high sensitivity and selectivity in concentration as high as 320 ng/mL,as well as a detection limit of 0.683 ng/mL This lanthanide-based microsphere holds great potential for rapid pointof-care screening and clinical application.

关键词： Eu(Ⅲ)chelate polystyrene microsphere Mini-emulsion polymerization Lateral flow immunoassays Alpha-fetoprotein Rare earths

Facile and Controllable Assembly of Multiwalled Carbon Nanotubes on polystyrene microspheres

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Polymer Science 2014年第6期32卷 711-717页

作者：李云兴 Yan Gao Cheng Yang Zhao-qun Wang Gi Xue The Key Laboratory of Food Colloids and Biotechnology Ministry of Education School of Chemical and Material Engineering Jiangnan University Department of Polymer Science and Engineering School of Chemistry and Chemical Engineering Nanjing University

Herein a facile and controllable heterocoagulation between polystyrene （PS） microspheres and multiwalled carbon nanotubes （MWCNTs） is introduced based on colloid thermodynamics. The MWCNTs play the role of steric stabilizer for stabilizing the metastable PS microspheres and thus immobilize spontaneously on the surface of PS microspheres. The synthesized MWCNTs-coated PS composite particles have been extensively characterized by scanning electron microscopy, transmission electron microscopy, thermogravimetry and Raman spectroscopy. The results indicate that the structure and morphology of the resultant MWCNTs-coated PS composite particles are significantly affected by the weight ratio of PS and MWNCTs and the amount of poly（vinylpyrrolidone） that is injected into PS dispersion before they are mixed with MWCNTs. Therefore, these composite particles have the potential to produce MWCNTs-based composite materials with controllable mass loading and dispersity of MWCNTs.

关键词： Multiwalled carbon nanotube polystyrene microsphere Heterocoagulation Structural control Colloidthermodynamics.

Preparation of microsized crosslinked microspheres by dispersion copolymerization of polystyrene/polyethylene glycol 200-dimethacrylae

在线全文

学校读者我要写书评

暂无评论

Journal of University of Science and Technology Beijing 2006年第4期13卷 380-384页

作者： Wenli Guo Juan Wang Tongxiang Liang Institute of Nuclear Energy Technology Tsinghua University Beijing 100084 China

Dispersion copolymerization of styrene with polyethylene glycol 200-dimethacrylae as the cross-linking agent was completed by using poly（N-vinyl pyrrolidone） and 2,2-azo-bisisobutyronitrile as the steric stabilizer and initiator, respectively. Crosslinked copolymeric microspheres were prepared directly by the one-step method of dispersion copolymerization. The effects of the content of polyethylene glycol 200-dimethacrylae on the particle morphology and the copolymerization rate were investigated. It shows that the crosslinking agent plays an important role in the particle morphology and the system stability. When the content of crosslinking reached 2.5wt%, the floriated particles were obtained.

关键词： polystyrene microsphere dispersion polymerization crosslinking agent

Fabrication of macroporous polystyrene/graphene oxide composite monolith and its adsorption property for tetracycline

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2016年第4期27卷 511-517页

作者： Le-Chen Chen Shan Lei Mo-Zhen Wang Jun Yang Xue-Wu Ge The USTC-Anhui Tobacco Joint Laboratory of Tobacco Chemistry CAS Key Laboratory of Soft Matter Chemistry Department of Polymer Science and Engineering University of Science and Technology of China The USTC-Anhui Tobacco Joint Laboratory of Tobacco Chemistry Research Center of Tobacco and Health University of Science and Technology of China

Macroporous polystyrene microsphere/graphene oxide（PS/GO） composite monolith was first prepared using Pickering emulsion droplets as the soft template. The Pickering emulsion was stabilized by PS/GO composite particles in-situ formed in an acidic water phase. With the evaporation of water and the oil phase（octane）, the Pickering emulsion droplets agglomerated and combined with each other, forming a three-dimensional macroporous PS/GO composite matrix with excellent mechanical strength. The size of the macrospores ranged from 4 mm to 20 mm. The macroporous PS/GO composite monolith exhibited high adsorption capacity for tetracycline（TC） in an aqueous solution at p H 4–6. The maximum adsorption capacity reached 197.9 mg g 1at p H 6. The adsorption behaviour of TC fitted well with the Langmuir model and pseudo-second-order kinetic model. This work offers a simple and efficient approach to fabricate macroporous GO-based monolith with high strength and adsorption ability for organic pollutants.

关键词： Graphene oxide polystyrene microsphere Macroporousm Adsorption Tetracycline

Application of Optical Tweezers in the Research of Molecular Interaction between Lymphocyte Function Associated Antigen-1 and Its Monoclonal Antibody

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2007年第3期4卷 221-225页

作者： Haodong Chen Kuikui Ge Yinmei Li Jianguang Wu Yongqiang Gu Haiming Wei Zhigang Tian Institute of Immunology Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences University of Science and Technology of China Hefei 230027 China Hefei National Laboratory for Physical Sciences at Microscale and Physics Department University of Science and Technology of China Hefei 230027 China

Lymphocyte function associated antigen-1 （CD11a/CD18, LFA-1） plays an important role in the structure of the immunological synapse and is required for efficient lysis of cytotoxic T lymphocytes （CTLs） and natural killer （NK） cells. To study the activation mode of LFA-1 on the NK cell surface, optical tweezers were used in the work. As an emerging technology, optical tweezers are widely used to manipulate microscopic objects and measure the forces of molecular interactions in the field of biological research. In our study, a new platform was constructed to study the single molecular behavior of receptor on cell surface using optical tweezers. Based on the platform, the interaction between an NK cell and a polystyrene microsphere coated with anti-LFA-1 antibody was observed. The result confirmed that the adhesion forces between an NK cell and a polystyrene bead were time-dependent. According to our findings, we propose that anti-LFA-1 antibody may cause the clustering of LFA-1 on NK cell surface. Cellular ＆ Molecular Immunology.

关键词： NK cell LFA- 1 optical tweezers polystyrene microsphere