Application of Optical Tweezers in the Research of Molecular Interaction between Lymphocyte Function Associated Antigen-1 and Its Monoclonal Antibody

作     者：Haodong Chen Kuikui Ge Yinmei Li Jianguang Wu Yongqiang Gu Haiming Wei Zhigang Tian 

作者机构：Institute of Immunology Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences University of Science and Technology of China Hefei 230027 China Hefei National Laboratory for Physical Sciences at Microscale and Physics Department University of Science and Technology of China Hefei 230027 China 

出 版 物：《Cellular & Molecular Immunology》 (中国免疫学杂志（英文版）)

年 卷 期：2007年第4卷第3期

页      面：221-225页

核心收录：

学科分类：0710[理学-生物学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学] 

基　　金：National Natural Science Foundation of China (No. 10474094  30371308) 

主　　题：NK cell LFA- 1 optical tweezers polystyrene microsphere 

摘      要：Lymphocyte function associated antigen-1 （CD11a/CD18, LFA-1） plays an important role in the structure of the immunological synapse and is required for efficient lysis of cytotoxic T lymphocytes （CTLs） and natural killer （NK） cells. To study the activation mode of LFA-1 on the NK cell surface, optical tweezers were used in the work. As an emerging technology, optical tweezers are widely used to manipulate microscopic objects and measure the forces of molecular interactions in the field of biological research. In our study, a new platform was constructed to study the single molecular behavior of receptor on cell surface using optical tweezers. Based on the platform, the interaction between an NK cell and a polystyrene microsphere coated with anti-LFA-1 antibody was observed. The result confirmed that the adhesion forces between an NK cell and a polystyrene bead were time-dependent. According to our findings, we propose that anti-LFA-1 antibody may cause the clustering of LFA-1 on NK cell surface. Cellular ＆ Molecular Immunology.