One-step polymerized lanthanide-based polystyrene microsphere for sensitive lateral flow immunoassay

作     者：Zhenhua Li Qingyun Liu Yongfang Li Wei Yuan Fuyou Y.Li Zhenhua Li;Qingyun Liu;Yongfang Li;Wei Yuan;Fuyou Y.Li

作者机构：State Key Laboratory of Molecular Engineering of PolymersDepartment of ChemistryFudan UniversityShanghai200433China 

出 版 物：《Journal of Rare Earths》 (稀土学报（英文版）)

年 卷 期：2021年第39卷第1期

页      面：11-18,I0001页

核心收录：

学科分类：081704[工学-应用化学] 1002[医学-临床医学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 100214[医学-肿瘤学] 0703[理学-化学] 10[医学] 

基　　金：Project supported by the National Key R&D Program of China(2017YFA0205100) 

主　　题：Eu(Ⅲ)chelate Polystyrene microsphere Mini-emulsion polymerization Lateral flow immunoassays Alpha-fetoprotein Rare earths 

摘      要：Lateral flow immunoassays(LFIAs)provide a powerful tool for rapid real-time assay of cancer biomarkers,which is vital for cancer detection and treatment ***-based LFIAs is one of the most widely used methods,especially Eu(Ⅲ)chelates,which possess distinctive and attractive characteristics,such as time-resolved fluorescence and large Stokes ***,we adopted a new onestep mini-emulsion polymerization method to synthesize carboxyl-modified fluorescent microsphere(OS-EuCM),which shows good stability,resistance to non-specific adhesion and uniform particle size distribution compared with traditional microspheres synthesized through the swelling *** from the above advantages,OS-EuCM was successfully used in LFIAs to detect tumor marker Alpha-fetoprotein with high sensitivity and selectivity in concentration as high as 320 ng/mL,as well as a detection limit of 0.683 ng/mL This lanthanide-based microsphere holds great potential for rapid pointof-care screening and clinical application.