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interspecific hybridization Between Crassostrea angulata and C. ariakensis

Journal of Ocean University of China 2015年第4期14卷 710-716页

作者： YAO Tuo ZHANG Yuehuan YAN Xiwu WANG Zhaoping LI Dongchun SU Jiaqi YU Ruihai Fisheries College Ocean University of China Key Laboratory of Tropical Marine Bio-resources and Ecology South China Sea Institute of Oceanology Chinese Academy of Sciences Engineering Research Center of Shellfish Culture and Breeding of Liaoning Province College of Fisheries and Life Science Dalian Ocean University

lnterspecific hybridization can generate heterosis, which is proven to be a useful tool in selective breeding programs for oyster culture. Crassostrea angulata and C. ariakensis are two important economic shellfish species in China. We conducted 2 × 2 reciprocal crosses to determine whether these two species can cross-fertilize and their hybrids can hatch, survive and perform hetero- sis. Fertilization was found symmetrical without delay. The rate of fertilization success of C. angulata ♀× C. ariakensis ♂ was lower than that of C. ariakensis ♀ x C. angulata ♂, and the success rate of both hybridizations was lower than that of two intraspecific crosses each. During the planktonic period, survival rate of the progeny was lower in the hybrid crosses than in the intraspecific crosses. On day 360, mean shell height of the progeny of C. angulata ♀ x C. angulata ♂ was highest, which was followed by that of C. angulata ♀ × C. ariakensis ♂, C. ariakensis ♀x C. ariakensis ♂ and C. ariakensis ♀× C. angulata ♂ in a descending order. Mor- phology of adults produced by the hybrid crosses was similar to that of C. angulata. Both hybrids underwent normal gonad devel- opment and produced mature gametes in the mating season. This study provided new insights into the quantitative traits in interspeci- fic crosses of Crassostrea species, thus being of guidance value for selective breeding of oyster.

关键词： Crassostrea angulata Crassostrea ariakensis interspecific hybridization gonad development heterosis

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Crossability Barriers in the interspecific hybridization between Oryza sativa and ***

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Journal of Integrative Plant Biology 2009年第1期51卷 21-28页

作者： Xue-Lin Fu Yong-Gen Lu Xiang-Dong Liu Jin-Quan Li Guangdong Provincial Key Laboratory of Plant Molecular Breeding South China Agricultural University Guangzhou 510642 China

Oryza meyeriana Baill （GG genome） is a precious germplasm in the tertiary gene pool of cultivated rice （AA genome）, and possesses important traits such as resistance and tolerance to biotic and abiotic stress. However, interspeciflc crossability barrier, a critical bottleneck restricting genes transfer from O. meyeriana to cultivars has led to no hybrids through conventional reproduction. Therefore, the reasons underlying incrossability were investigated in the present report. The results showed that：（i） at 3-7 d after pollination （DAP）, many hybrid embryos degenerated at the earlier globular-shaped stage, and could not develop into the later pear-shaped stage. Meanwhile, free endosperm nuclei started to degenerate at 1 DAP, and cellular endosperm could not form at 3 DAP, leading to nutrition starvation for young embryo development; （ii） at 11-13 DAP, almost all hybrid ovaries aborted. Even though 72.22% of hybrid young embryos were produced in the interspecific hybridization between O. sativa and O. meyeriana, young embryos were not able to further develop into hybrid plantlets via culturing in vitro. The main reason for the incrossability was hybrid embryo inviability, presenting as embryo development stagnation and degeneration since 3 DAP. Some possible approaches to overcome the crossability barriers in the interspecific hybridization between O. sativa and O. meyeriana are discussed.

关键词： crossability embryo development interspecific hybridization Oryza sativa O. meyeriana.

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Progress on Transferring Elite Genes from Non-AA Genome Wild Rice into Oryza sativa through interspecific hybridization

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Rice science 2008年第2期15卷 79-87页

作者： Fu Xue-lin LU Yong-gen LIU Xiang-dong LI Jin-quan Guangdong Provincial Key Laboratory of Plant Molecular Breeding South China Agricultural University Guangzhou 510642 China

The progress of research on transferring elite genes from non-AA genome wild rice into Oryza sativa through interspecific hybridization are in three respects, that is, breeding monosomic alien addition lines （MAALs）, constructing introgression lines （ILs） and analyzing the heredity of the characters and mapping the related genes. There are serious reproductive barriers, mainly incrossability and hybrid sterility, in the interspecific hybridization of O. sativa with non-AA genome wild rice. These are the ＇bottleneck＇ for transferring elite genes from wild rice to O. sativa. Combining traditional crossing method with biotechnique is a reliable way to overcome the reproductive barriers and to improve the utilizing efficiency of non-AA genome wild rice.

关键词： Oryza sativa non-AA genome wild rice interspecific hybridization monosomic alien addition line introgression line reproductive barrier gene mapping

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Cytogenetic Analysis of the Primary Amphidiploid Derived from interspecific hybridization in Cucumis and Its Selfed Progenies

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Agricultural Sciences in China 2005年第5期4卷 336-342页

作者： CHENJin-feng QIANChun-tao JEStaub LUOXiang-dong ZHUANGFei-yun NationalKeyLaboratoryofCropGeneticsandGermplasmEnhancement CollegeofHorticultureNanjingAgriculturalUniversityNanjing210095P.R.China USDA／ARS DepartmentofHorticultureUniversityofWisconsinMadisonWI53706USA

Studies on the reproduction and cytogenetic characterization of a primary amphidiploid Cucumis species C. hytivus Chen and Kirkbride (2n = 4x = 38) indicated that a more comprehensive cytogenetic analysis of this species and its first selfed progeny would increase its potential utility in cucumber improvement. With tendrils used as source materials for mitotic analysis, chromosome numbers in all selfed progenies were 2n = 38, confirming chromosomal stability in this synthetic amphidiploid species. Detailed meiotic processes were described by comparing the primary and the selfed amphidiploids. Meiotic abnormalities, such as chromosome lagging, unequal separation, chromosome multi-polarization and polyads were observed frequently in all amphidiploid plants except for the selfed no.8, in which meiosis was arrested prior to metaphase Ⅰ. Generally, the frequency of multivalents was higher and the configurations were more complex in the selfed progenies, demonstrating a more extensive genetic exchange between cucumber and C. hystrix Chakr. Genome separation between cucumber and C. hystrix was observed through prophase Ⅰ to anaphase Ⅰ in both generations of the amphidiploids. Consequently, in addition to n = 19, a new gamete with n = 7 was produced, which was confirmed by the chromosome counts 2n = 14 in the backcrossing progenies from cucumber × amphidiploid mating. Fertility varied among the selfed amphidiploid plants. The selfed plant no.1 was found to have an improved fertility (e.g., pollen staining ability 40.8% and 25.6 seeds per fruit) and then was used as source germplasm in further introgression and gene exchange experiments.

关键词： interspecific hybridization Cucumis Chromosome pairing Genome separation Amphidiploid

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interspecific hybridization between two oyster species,Crassostrea hongkongensis and C. angulata

Interspecific hybridization between two oyster species,Crass...

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中国动物学会·中国海洋湖沼学会贝类学分会第九次会员代表大会暨第十五次学术讨论会

作者： Yuehuan Zhang1,2,Zhaoping Wang1,*a Xiwu Yan2 Zhifei Yu2 Ruihai Yu3 Zhongming She3 1.Ocean University of China,Fisheries College,Qingdao 266003 2.College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023 3.Shenzhen Bao An Fisheries Research Institute,Shenzhen,518104

interspecific hybridization can generate transgressive hybrid phenotypes with extreme trait values exceeding the combined range of the parental *** understand potential heterosis between Crassostrea hongjongensis and ***,three successful replicates of 2×2 factorial cross experiments were conducted between these two species on May 2011 in Shenzhen(Guangdong Province).The results revealed an asymmetry in fertilization;*** eggs could be fertilized by *** sperm,and fertilization success in *** female×*** male(HA) crosses were significantly lower than the two intraspecific *** larvae had a slower growth rate than these of two parental species because of genome *** weakness of growth was observed in larval stage,whereas spat growth heterosis was negative in Dalian but positive in Shenzhen during grow out *** results may be come from difference in temperature between South and North *** survival heterosis of hybrids was examined all the time excepted day 3,day6 and *** analysis by complex COI and ITS confirmed that the HA spat were true *** of hybrids was generally functional sterile,only a small of them was *** analysis demonstrates that interspecific hybridization between female *** and male *** may be a promising method for genetic improvement of oysters in South China.

关键词： Crassotrea gigas C.hongkongensis interspecific hybridization heterosis Hybrid sterile

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interspecific hybridization between Cucumis ssp. Plants and SRAP Analysis of Hybrids

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Agricultural Science & Technology 2011年第8期12卷 1118-1120,1128页

作者：王吉明包文风尚建立马双武樊辉中国农业科学院郑州果树研究所河南郑州450009

To understand the inter-hybridization between Cucumis ***,we used 150 melon varieties as female parents to cross with Cucumis metuliferus and Cucumis *** melon accessions V2 and V129 set fruits,but seeds from fruits V2（V129）×*** were abortive.A few of seeds from the bottom of fruit V2（V129）×*** were ***-related amplified polymorphism（SRAP）molecular markers were used to analyze the progenies of inter-specific hybridization between *** and melon *** pair primer（E14/M2）was found effective in amplification on male parent characteristic bands from the hybrids,suggesting that some DNA exchange had happened between *** and melon *** study provided data for analyzing the mechanism of inter-hybridization between Cucumis plants.

关键词： Cucumis ssp. interspecific hybridization SRAP Markers

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Induction of Tetraploid Male Sterile Tagetes erecta by Colchicine Treatment and Its Application for interspecific hybridization

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Horticultural Plant Journal 2016年第5期2卷 284-292页

作者： HE Yanhong SUN Yalin ZHENG Riru AI Ye CAO Zhe BAO Manzhu Key Laboratory of Horticultural Plant Biology Ministry of Education College of Horticulture and Forestry Sciences Huazhong Agricultural University Institute of Vegetable Science Wuhan Academy of Agricultural Sciences College of Landscape Architecture Fujian Agriculture and Forestry University Gulf Coast Research and Education Center Institute of Food and Agricultural Sciences University of Florida

Tagetes erecta is an annual multifunctional plant which can be cultivated under a broad range of climatic conditions. Polyploidization and interspecific hybridization are applied to facilitate breeding cultivars of T. erecta with improved ornamental qualities. Colchicine treatment to the germinating seeds was proved to be a useful tool for chromosome doubling of the male sterile two-type line ‘M525AB', with the resulting frequency of polyploid seedlings ranging from 88.89%(following 0.05% w/v colchicine applied for a 3–6 h exposure period) to a maximum of100.00%(following 0.1% for 3–6 h, or 0.2% for 3 h). Morphological observation, stomatal size and density analysis, flow cytometric analysis and chromosome counting were conducted to identify the tetraploid plants. Distinctive morphological changes were observed in a notable proportion of polyploid plants. The colchicine-treated polyploid T. erecta plants showed dwarfed and more robust growth, thicker, larger and greener leaves, larger inflorescences and florets. The mutant plants identified through morphological observation all aligned as polyploid plants, thus morphological observation could be an effective method for the detection of polyploidy. The polyploid plants had significant larger stomata size over the abaxial leaf surface, whereas the density of stomata distribution was remarkably reduced. The survival rate of tetraploid cuttings(i.e. 38%)was greatly reduced compared to that of diploid plants. The fertility of tetraploid plants was also decreased, as shown by cross-pollination *** hybridizations between colchicine-induced tetraploid plants of a male sterile T. erecta line and the naturally tetraploid fully fertile Tagetes patula species resulted in hybrid progeny. Most of these hybrids displayed the dwarfed growth stature and compact, larger-flower morphology which is the typical ideotype of herbaceous flowers. Thus, polyploidization may be employed effectively as a means to facilitate in

关键词： Tagetes erecta Tagetes patula chromosome doubling colchicine tetraploid interspecific hybridization

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Artificial interspecific hybridization between two oyster species,Crassostrea hongkongensis and C. gigas

Artificial interspecific hybridization between two oyster sp...

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中国动物学会·中国海洋湖沼学会贝类学分会第九次会员代表大会暨第十五次学术讨论会

作者： Yuehuan Zhang~(1,2) Zhaoping Wang~(1,*a) Xiwu Yan~2, Ruihai Yu~1 Jing Kong~1 Jian Liu~1 Ximing Guo~(3,*b) 1.Ocean University of China,Fisheries College,Qingdao 266003 2.College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023 3.Haskin Shellfish Research Laboratory,Institute of Marine and Coastal Sciences, Rutgers University,Port Norris,New Jersey 08349

The cupped-oyster species Crassostrea hongkongensis and *** are economically and ecologically important species native to the South and North coasts of China,*** ascertain the feasibility of interspecific hybridization between these two species,131 times 2×2 factorial cross experiments were conducted from May 2009 to December *** results revealed an asymmetry in fertilization;*** eggs could be fertilized by *** sperm,while the reciprocal cross did not result in *** success in C. hongkongensis female×*** male(HG) crosses were always lower than the two intraspecific crosses,and the HG larvae had a growth rate similar to the maternal species during the first several days of development because of maternal ***,genome incompatibility suppressed on growth rates of *** weakness of growth was observed at all times, whereas survival heterosis was fluctuant under diverse commercial culture *** analysis confirmed that the HG spat contained DNA from both species and thus were true hybrids. When hybrids grew to sex mature by one year culture,we observed that most of hybrids were generally functional sterile,but with a small proportion being *** analysis explicitly demonstrates that interspecific hybridization between *** and *** is possible in one direction and their hybrids are generally sterile,resulting in partial reproductive isolation in postmating and postzygotic isolation.

关键词： Crassotrea hongkongensis C.gigas interspecific hybridization heterosis hybrid sterile

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Comparative Embryological Studies on Infertility of interspecific hybridizations Between Oryza sativa with Different Ploidy Levels and O. officinalis

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Rice science 2009年第1期16卷 58-64页

作者： FU Xue-lin LU Yong-gen LIU Xiang-dong LI Jin-quan ZHAO Xing-juan Guangdong Provincial Key Laboratory of Plant Molecular Breeding South China Agricultural University Guangzhou 510642 China

As maternal parents, diploid （L202-2x） and autotetraploid （L202-4x） of Oryza sativa cv. L2O2 were crossed with O. officinalis. Embryo development and fertilization in these two crosses were comparatively studied. There were no mature hybrid seeds obtained because all the hybridized spikelets died 30 days after pollination. The main reasons for no seed set were abnormal fertilization and development of the embryos and endosperms in the interspecific hybrids. There were doublefertilization, egg cell single-fertilization and non-fertilization in these crosses. Although 59.45% and 54.87% of hybrid embryos produced in the crosses of L202-2x/O. officinalis and L202-4x/O. officinalis, respectively, hybrid embryos ceased to develop or degenerated and plenty of free endosperm nuclei were in disaggregating state without developing cellular endosperms three days after pollination. Besides, some embryological differences in these two crosses were found, that is, the rate of double-fertilization and total rate of doubleand single-fertilization in L202-2x/O. officinalis were higher than those in L202-4x/O. officinalis. The embryo and endosperm of hybrids developed more slowly, and embryos and free endosperm nuclei were more severely degenerated in L202-4x/O. officinalis than in L202-2x/O. officinalis. Five days after pollination, a few of embryos in L202-2x/O. officinalis developed into pear-shaped ones, however, embryos in L202-4x/O. officinalis were all degenerated. Therefore, it is more difficult to obtain interspecific hybrids by wide crosses between autotetraploid of O. sativa and O. officinalis.

关键词： Oryza sativa Oryza officinalis diploid rice autotetraploid rice wild rice interspecific hybridization embryo development infertility

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A hybrid sterile locus leads to the linkage drag of interspecific hybrid progenies

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Plant Diversity 2020年第5期42卷 370-375页

作者： Mianmian Wang Jun Yang Jinpeng Wan Dayun Tao Jiawu Zhou Diqiu Yu Peng Xu Key Laboratory of Tropical Plant Resources and Sustainable Use Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesKunming650223YunnanChina University of Chinese Academy of Sciences Beijing100049China Center of Economic Botany Core Botanical GardensChinese Academy of SciencesMenglunMengla666303YunnanChina Yunnan Key Laboratory for Rice Genetic Improvement Food Crops Research InstituteYunnan Academy of Agricultural SciencesKunming650200YunnanChina

interspecific hybridization plays an important role in rice breeding by broadening access to desirable traits such as disease resistance and improving ***,interspecific hybridization is often hindered by hybrid sterility,linkage drag,and distorted *** mine for favorable genes from Oryza glaberrima,we cultivated a series of BC4 introgression lines(ILs)of *** in the japonica rice variety background(Dianjingyou 1)in which the IL-2769(BC4F10)showed longer sterile lemmas,wider grains and spreading panicles compared with its receptor parent,suggesting that linkage drag may have *** on the BC5F2 population,a hybrid sterility locus,S20,a long sterile lemma locus,G1-g,and a new grain width quantitative trait locus(QTL),qGW7,were mapped in the linkage region about 15 centimorgan(cM)from the end of the short arm of chromosome *** hybrid sterility locus S20 from *** eliminated male gametes of Oryza sativa,and male gametes carrying the alleles of *** in the heterozygotes were aborted *** addition,the homozygotes presented a genotype of ***,and homozygous *** were not ***,the linked traits G1-g and qGW7 showed similar segregation *** results indicate that S20 was responsible for the linkage *** a large number of detected hybrid sterility loci are widely distributed on rice chromosomes,we suggest that hybrid sterility loci are the critical factors for the linkage drag in interspecific and subspecific hybridization of rice.

关键词： interspecific hybridization Linkage drag Hybrid sterility Segregation distortion

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