Comparative Embryological Studies on Infertility of Interspecific Hybridizations Between Oryza sativa with Different Ploidy Levels and O. officinalis

作     者：FU Xue-lin LU Yong-gen LIU Xiang-dong LI Jin-quan ZHAO Xing-juan FU Xue-lin, LU Yong-gen, LIU Xiang-dong, LI Jin-quan, ZHAO Xing-juan (Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, China)

作者机构：Guangdong Provincial Key Laboratory of Plant Molecular Breeding South China Agricultural University Guangzhou 510642 China 

出 版 物：《Rice science》 (水稻科学（英文版）)

年 卷 期：2009年第16卷第1期

页      面：58-64页

学科分类：0710[理学-生物学] 0907[农学-林学] 07[理学] 08[工学] 0829[工学-林业工程] 09[农学] 071002[理学-动物学] 

基　　金：supported by the Guangdong Provincial Key Project of Natural Science Foundation, China (Grant No. 021037) Guangdong Province Natural Science Foundation, China (Grant No. 7301008) South China Agricultural University President Foundation, China (Grant No. 2007K036) The Confocal Laser Scanning Microscope was provided by the Testing Center of South China Agricultural University, Guangzhou, China 

主　　题：Oryza sativa Oryza officinalis diploid rice autotetraploid rice wild rice interspecific hybridization embryo development infertility 

摘      要：As maternal parents, diploid （L202-2x） and autotetraploid （L202-4x） of Oryza sativa cv. L2O2 were crossed with O. officinalis. Embryo development and fertilization in these two crosses were comparatively studied. There were no mature hybrid seeds obtained because all the hybridized spikelets died 30 days after pollination. The main reasons for no seed set were abnormal fertilization and development of the embryos and endosperms in the interspecific hybrids. There were doublefertilization, egg cell single-fertilization and non-fertilization in these crosses. Although 59.45% and 54.87% of hybrid embryos produced in the crosses of L202-2x/O. officinalis and L202-4x/O. officinalis, respectively, hybrid embryos ceased to develop or degenerated and plenty of free endosperm nuclei were in disaggregating state without developing cellular endosperms three days after pollination. Besides, some embryological differences in these two crosses were found, that is, the rate of double-fertilization and total rate of doubleand single-fertilization in L202-2x/O. officinalis were higher than those in L202-4x/O. officinalis. The embryo and endosperm of hybrids developed more slowly, and embryos and free endosperm nuclei were more severely degenerated in L202-4x/O. officinalis than in L202-2x/O. officinalis. Five days after pollination, a few of embryos in L202-2x/O. officinalis developed into pear-shaped ones, however, embryos in L202-4x/O. officinalis were all degenerated. Therefore, it is more difficult to obtain interspecific hybrids by wide crosses between autotetraploid of O. sativa and O. officinalis.