Cytogenetic Analysis of the Primary Amphidiploid Derived from Interspecific Hybridization in Cucumis and Its Selfed Progenies

作     者：CHENJin-feng QIANChun-tao JEStaub LUOXiang-dong ZHUANGFei-yun 

作者机构：NationalKeyLaboratoryofCropGeneticsandGermplasmEnhancementCollegeofHorticultureNanjingAgriculturalUniversityNanjing210095P.R.China USDA／ARSDepartmentofHorticultureUniversityofWisconsinMadisonWI53706USA 

出 版 物：《Agricultural Sciences in China》 (中国农业科学（英文版）)

年 卷 期：2005年第4卷第5期

页      面：336-342页

学科分类：09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：This research was partially supported by the Trans-century Training Program Foundation for the Talents by the State Education Commission of China to Dr.Chen Jinfeng,by the National Natural Science Founda-tion of China(301 70644 and 30470120) by National Hi-Tech R&D Program(2002AA241251 and 2004 AA241120) by Tang's Cornell-China Scholar Program and by Pickle Seed Research Foundation of PPI 

主　　题：Interspecific hybridization Cucumis Chromosome pairing Genome separation Amphidiploid 

摘      要：Studies on the reproduction and cytogenetic characterization of a primary amphidiploid Cucumis species C. hytivus Chen and Kirkbride (2n = 4x = 38) indicated that a more comprehensive cytogenetic analysis of this species and its first selfed progeny would increase its potential utility in cucumber improvement. With tendrils used as source materials for mitotic analysis, chromosome numbers in all selfed progenies were 2n = 38, confirming chromosomal stability in this synthetic amphidiploid species. Detailed meiotic processes were described by comparing the primary and the selfed amphidiploids. Meiotic abnormalities, such as chromosome lagging, unequal separation, chromosome multi-polarization and polyads were observed frequently in all amphidiploid plants except for the selfed no.8, in which meiosis was arrested prior to metaphase Ⅰ. Generally, the frequency of multivalents was higher and the configurations were more complex in the selfed progenies, demonstrating a more extensive genetic exchange between cucumber and C. hystrix Chakr. Genome separation between cucumber and C. hystrix was observed through prophase Ⅰ to anaphase Ⅰ in both generations of the amphidiploids. Consequently, in addition to n = 19, a new gamete with n = 7 was produced, which was confirmed by the chromosome counts 2n = 14 in the backcrossing progenies from cucumber × amphidiploid mating. Fertility varied among the selfed amphidiploid plants. The selfed plant no.1 was found to have an improved fertility (e.g., pollen staining ability 40.8% and 25.6 seeds per fruit) and then was used as source germplasm in further introgression and gene exchange experiments.