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Progress on Transferring Elite Genes from Non-AA Genome Wild Rice into Oryza sativa through interspecific hybridization

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Rice science 2008年第2期15卷 79-87页

作者： Fu Xue-lin LU Yong-gen LIU Xiang-dong LI Jin-quan Guangdong Provincial Key Laboratory of Plant Molecular Breeding South China Agricultural University Guangzhou 510642 China

The progress of research on transferring elite genes from non-AA genome wild rice into Oryza sativa through interspecific hybridization are in three respects, that is, breeding monosomic alien addition lines （MAALs）, constructing introgression lines （ILs） and analyzing the heredity of the characters and mapping the related genes. There are serious reproductive barriers, mainly incrossability and hybrid sterility, in the interspecific hybridization of O. sativa with non-AA genome wild rice. These are the ＇bottleneck＇ for transferring elite genes from wild rice to O. sativa. Combining traditional crossing method with biotechnique is a reliable way to overcome the reproductive barriers and to improve the utilizing efficiency of non-AA genome wild rice.

关键词： Oryza sativa non-AA genome wild rice interspecific hybridization monosomic alien addition line introgression line reproductive barrier gene mapping

Cytogenetic Analysis of the Primary Amphidiploid Derived from interspecific hybridization in Cucumis and Its Selfed Progenies

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Agricultural Sciences in China 2005年第5期4卷 336-342页

作者： CHENJin-feng QIANChun-tao JEStaub LUOXiang-dong ZHUANGFei-yun NationalKeyLaboratoryofCropGeneticsandGermplasmEnhancement CollegeofHorticultureNanjingAgriculturalUniversityNanjing210095P.R.China USDA／ARS DepartmentofHorticultureUniversityofWisconsinMadisonWI53706USA

Studies on the reproduction and cytogenetic characterization of a primary amphidiploid Cucumis species C. hytivus Chen and Kirkbride (2n = 4x = 38) indicated that a more comprehensive cytogenetic analysis of this species and its first selfed progeny would increase its potential utility in cucumber improvement. With tendrils used as source materials for mitotic analysis, chromosome numbers in all selfed progenies were 2n = 38, confirming chromosomal stability in this synthetic amphidiploid species. Detailed meiotic processes were described by comparing the primary and the selfed amphidiploids. Meiotic abnormalities, such as chromosome lagging, unequal separation, chromosome multi-polarization and polyads were observed frequently in all amphidiploid plants except for the selfed no.8, in which meiosis was arrested prior to metaphase Ⅰ. Generally, the frequency of multivalents was higher and the configurations were more complex in the selfed progenies, demonstrating a more extensive genetic exchange between cucumber and C. hystrix Chakr. Genome separation between cucumber and C. hystrix was observed through prophase Ⅰ to anaphase Ⅰ in both generations of the amphidiploids. Consequently, in addition to n = 19, a new gamete with n = 7 was produced, which was confirmed by the chromosome counts 2n = 14 in the backcrossing progenies from cucumber × amphidiploid mating. Fertility varied among the selfed amphidiploid plants. The selfed plant no.1 was found to have an improved fertility (e.g., pollen staining ability 40.8% and 25.6 seeds per fruit) and then was used as source germplasm in further introgression and gene exchange experiments.

关键词： interspecific hybridization Cucumis Chromosome pairing Genome separation Amphidiploid

interspecific hybridization between two oyster species,Crassostrea hongkongensis and C. angulata

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Interspecific hybridization between two oyster species,Crass...

Induction of Tetraploid Male Sterile Tagetes erecta by Colchicine Treatment and Its Application for interspecific hybridization

中国动物学会·中国海洋湖沼学会贝类学分会第九次会员代表...

作者： Yuehuan Zhang1,2,Zhaoping Wang1,*a Xiwu Yan2 Zhifei Yu2 Ruihai Yu3 Zhongming She3 1.Ocean University of China,Fisheries College,Qingdao 266003 2.College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023 3.Shenzhen Bao An Fisheries Research Institute,Shenzhen,518104

interspecific hybridization can generate transgressive hybrid phenotypes with extreme trait values exceeding the combined range of the parental *** understand potential heterosis between Crassostrea hongjongensis and ***,three successful replicates of 2×2 factorial cross experiments were conducted between these two species on May 2011 in Shenzhen(Guangdong Province).The results revealed an asymmetry in fertilization;*** eggs could be fertilized by *** sperm,and fertilization success in *** female×*** male(HA) crosses were significantly lower than the two intraspecific *** larvae had a slower growth rate than these of two parental species because of genome *** weakness of growth was observed in larval stage,whereas spat growth heterosis was negative in Dalian but positive in Shenzhen during grow out *** results may be come from difference in temperature between South and North *** survival heterosis of hybrids was examined all the time excepted day 3,day6 and *** analysis by complex COI and ITS confirmed that the HA spat were true *** of hybrids was generally functional sterile,only a small of them was *** analysis demonstrates that interspecific hybridization between female *** and male *** may be a promising method for genetic improvement of oysters in South China.

关键词： Crassotrea gigas C.hongkongensis interspecific hybridization heterosis Hybrid sterile

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Horticultural Plant Journal 2016年第5期2卷 284-292页

作者： HE Yanhong SUN Yalin ZHENG Riru AI Ye CAO Zhe BAO Manzhu Key Laboratory of Horticultural Plant Biology Ministry of Education College of Horticulture and Forestry Sciences Huazhong Agricultural University Institute of Vegetable Science Wuhan Academy of Agricultural Sciences College of Landscape Architecture Fujian Agriculture and Forestry University Gulf Coast Research and Education Center Institute of Food and Agricultural Sciences University of Florida

Tagetes erecta is an annual multifunctional plant which can be cultivated under a broad range of climatic conditions. Polyploidization and interspecific hybridization are applied to facilitate breeding cultivars of T. erecta with improved ornamental qualities. Colchicine treatment to the germinating seeds was proved to be a useful tool for chromosome doubling of the male sterile two-type line ‘M525AB', with the resulting frequency of polyploid seedlings ranging from 88.89%(following 0.05% w/v colchicine applied for a 3–6 h exposure period) to a maximum of100.00%(following 0.1% for 3–6 h, or 0.2% for 3 h). Morphological observation, stomatal size and density analysis, flow cytometric analysis and chromosome counting were conducted to identify the tetraploid plants. Distinctive morphological changes were observed in a notable proportion of polyploid plants. The colchicine-treated polyploid T. erecta plants showed dwarfed and more robust growth, thicker, larger and greener leaves, larger inflorescences and florets. The mutant plants identified through morphological observation all aligned as polyploid plants, thus morphological observation could be an effective method for the detection of polyploidy. The polyploid plants had significant larger stomata size over the abaxial leaf surface, whereas the density of stomata distribution was remarkably reduced. The survival rate of tetraploid cuttings(i.e. 38%)was greatly reduced compared to that of diploid plants. The fertility of tetraploid plants was also decreased, as shown by cross-pollination *** hybridizations between colchicine-induced tetraploid plants of a male sterile T. erecta line and the naturally tetraploid fully fertile Tagetes patula species resulted in hybrid progeny. Most of these hybrids displayed the dwarfed growth stature and compact, larger-flower morphology which is the typical ideotype of herbaceous flowers. Thus, polyploidization may be employed effectively as a means to facilitate in

关键词： Tagetes erecta Tagetes patula chromosome doubling colchicine tetraploid interspecific hybridization

Artificial interspecific hybridization between two oyster species,Crassostrea hongkongensis and C. gigas

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Artificial interspecific hybridization between two oyster sp...

Contemporary Asymmetric Genetic Introgression Between Two Pelophylax Species in Shanghai

中国动物学会·中国海洋湖沼学会贝类学分会第九次会员代表...

作者： Yuehuan Zhang~(1,2) Zhaoping Wang~(1,*a) Xiwu Yan~2, Ruihai Yu~1 Jing Kong~1 Jian Liu~1 Ximing Guo~(3,*b) 1.Ocean University of China,Fisheries College,Qingdao 266003 2.College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023 3.Haskin Shellfish Research Laboratory,Institute of Marine and Coastal Sciences, Rutgers University,Port Norris,New Jersey 08349

＜正＞The cupped-oyster species Crassostrea hongkongensis and *** are economically and ecologically important species native to the South and North coasts of China,*** ascertain the feasibility of interspecific hybridization between these two species,131 times 2×2 factorial cross experiments were conducted from May 2009 to December *** results revealed an asymmetry in fertilization;*** eggs could be fertilized by *** sperm,while the reciprocal cross did not result in *** success in C. hongkongensis female×*** male（HG） crosses were always lower than the two intraspecific crosses,and the HG larvae had a growth rate similar to the maternal species during the first several days of development because of maternal ***,genome incompatibility suppressed on growth rates of *** weakness of growth was observed at all times, whereas survival heterosis was fluctuant under diverse commercial culture *** analysis confirmed that the HG spat contained DNA from both species and thus were true hybrids. When hybrids grew to sex mature by one year culture,we observed that most of hybrids were generally functional sterile,but with a small proportion being *** analysis explicitly demonstrates that interspecific hybridization between *** and *** is possible in one direction and their hybrids are generally sterile,resulting in partial reproductive isolation in postmating and postzygotic isolation.

关键词： Crassotrea hongkongensis C.gigas interspecific hybridization heterosis hybrid sterile

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Asian Herpetological Research 2024年第1期15卷 1-11页

作者： Xu WEI Wenli LIU Gang WANG Rui MA Meiling HUANG Jiaxin ZHENG Tingwei DONG Changqing YUAN Shunqi BO Xiao YUAN Ben LI Shuo MA Qu YUE Youzhong DING Zhenghuan WANG School of Life Sciences East China Normal UniversityShanghai 200241China Shanghai Landscaping and City Appearance Administrative Bureau Shanghai 200041China State Key Laboratory of Estuarine and Coastal Research East China Normal UniversityShanghai 200241China Shanghai Key Lab for Urban Ecological Processes and Eco-Restoration East China Normal UniversityShanghai 200241China Yangtze Delta Estuarine Wetland Ecosystem Observation and Research Station Ministry of Education&Shanghai Science and Technology CommitteeShanghai 202162China

Human disturbances are considered to break reproduction barriers among *** increases in hybridization events have been reported among a large number of taxonomic groups in anthropogenic environments,providing novel insights into species evolution mechanisms and conservation management in the *** Eastern Golden Frog(Pelophylax plancyi)and BlackSpotted Frog(***)are two sympatric anuran species with a long history of mitochondrial genome introgression in highly urbanized continental East ***,there is only limited understanding of the pattern of their contemporary hybridization and factors influencing their interspecific relationship under anthropogenic ***,interspecific hybridization between *** and *** at the population level was investigated in *** except two haplotypes obtained from both species in Shanghai were mixed together,and located in the introgression clade,implying multiple ancient mitochondrial introgression events occurred in the populations of our study *** genetic introgression was detected by microsatellite markers,with 0.7%of *** and 14.6%of *** identified as contemporary admixed *** with the trend of population density,higher genetic diversity of neutral microsatellite loci was found in the more abundant ***;however,variation in mitochondrial(Cyt-b)and nuclear(POMC)genes was higher in relatively rare *** population density of *** and number of water patches within local habitats were significantly positively correlated with both occurrences and proportions of admixed individuals in the populations of *** and *** the prevalent transformation of habitats in urbanized areas,these results imply that a high population density in isolated artificially altered habitats is likely to increase interspecific ***,population monitoring and improveme

关键词： anthropogenic disturbances interspecific hybridization Pelophylax species abundance urban landscape urbanization

Comparative Embryological Studies on Infertility of interspecific hybridizations Between Oryza sativa with Different Ploidy Levels and O. officinalis

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Rice science 2009年第1期16卷 58-64页

作者： FU Xue-lin LU Yong-gen LIU Xiang-dong LI Jin-quan ZHAO Xing-juan Guangdong Provincial Key Laboratory of Plant Molecular Breeding South China Agricultural University Guangzhou 510642 China

As maternal parents, diploid （L202-2x） and autotetraploid （L202-4x） of Oryza sativa cv. L2O2 were crossed with O. officinalis. Embryo development and fertilization in these two crosses were comparatively studied. There were no mature hybrid seeds obtained because all the hybridized spikelets died 30 days after pollination. The main reasons for no seed set were abnormal fertilization and development of the embryos and endosperms in the interspecific hybrids. There were doublefertilization, egg cell single-fertilization and non-fertilization in these crosses. Although 59.45% and 54.87% of hybrid embryos produced in the crosses of L202-2x/O. officinalis and L202-4x/O. officinalis, respectively, hybrid embryos ceased to develop or degenerated and plenty of free endosperm nuclei were in disaggregating state without developing cellular endosperms three days after pollination. Besides, some embryological differences in these two crosses were found, that is, the rate of double-fertilization and total rate of doubleand single-fertilization in L202-2x/O. officinalis were higher than those in L202-4x/O. officinalis. The embryo and endosperm of hybrids developed more slowly, and embryos and free endosperm nuclei were more severely degenerated in L202-4x/O. officinalis than in L202-2x/O. officinalis. Five days after pollination, a few of embryos in L202-2x/O. officinalis developed into pear-shaped ones, however, embryos in L202-4x/O. officinalis were all degenerated. Therefore, it is more difficult to obtain interspecific hybrids by wide crosses between autotetraploid of O. sativa and O. officinalis.

关键词： Oryza sativa Oryza officinalis diploid rice autotetraploid rice wild rice interspecific hybridization embryo development infertility

A hybrid sterile locus leads to the linkage drag of interspecific hybrid progenies

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Plant Diversity 2020年第5期42卷 370-375页

作者： Mianmian Wang Jun Yang Jinpeng Wan Dayun Tao Jiawu Zhou Diqiu Yu Peng Xu Key Laboratory of Tropical Plant Resources and Sustainable Use Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesKunming650223YunnanChina University of Chinese Academy of Sciences Beijing100049China Center of Economic Botany Core Botanical GardensChinese Academy of SciencesMenglunMengla666303YunnanChina Yunnan Key Laboratory for Rice Genetic Improvement Food Crops Research InstituteYunnan Academy of Agricultural SciencesKunming650200YunnanChina

interspecific hybridization plays an important role in rice breeding by broadening access to desirable traits such as disease resistance and improving ***,interspecific hybridization is often hindered by hybrid sterility,linkage drag,and distorted *** mine for favorable genes from Oryza glaberrima,we cultivated a series of BC4 introgression lines(ILs)of *** in the japonica rice variety background(Dianjingyou 1)in which the IL-2769(BC4F10)showed longer sterile lemmas,wider grains and spreading panicles compared with its receptor parent,suggesting that linkage drag may have *** on the BC5F2 population,a hybrid sterility locus,S20,a long sterile lemma locus,G1-g,and a new grain width quantitative trait locus(QTL),qGW7,were mapped in the linkage region about 15 centimorgan(cM)from the end of the short arm of chromosome *** hybrid sterility locus S20 from *** eliminated male gametes of Oryza sativa,and male gametes carrying the alleles of *** in the heterozygotes were aborted *** addition,the homozygotes presented a genotype of ***,and homozygous *** were not ***,the linked traits G1-g and qGW7 showed similar segregation *** results indicate that S20 was responsible for the linkage *** a large number of detected hybrid sterility loci are widely distributed on rice chromosomes,we suggest that hybrid sterility loci are the critical factors for the linkage drag in interspecific and subspecific hybridization of rice.

关键词： interspecific hybridization Linkage drag Hybrid sterility Segregation distortion

High-resolution linkage and quantitative trait locus mapping using an interspecific cross between Argopecten irradians irradians(♀) and A. purpuratus (♂)

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Marine Life Science & Technology 2020年第2期2卷 123-134页

作者： Junxia Mao Qifan Zeng Zujing Yang Haoyuan Pan Lijie Yao Zhenmin Bao Chunde Wang Shi Wang MOE Key Laboratory of Marine Genetics and Breeding College of Marine Life SciencesOcean University of ChinaQingdao 266003China Key Laboratory of Mariculture and Stock Enhancement in North China's Sea Ministry of Agriculture and Rural AffairsDalian Ocean UniversityDalian 116023China Laboratory for Marine Biology and Biotechnology Pilot National Laboratory for Marine Science and TechnologyQingdao 266237China Laboratory for Marine Fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and TechnologyQingdao 266237China Marine Science and Engineering College Qingdao Agricultural UniversityQingdao 266109China

The bay scallop and Peruvian scallop are economically important *** hybrids of these two scallops outperformed both of their parent species in multiple growth traits but exhibited decreased fertility,which provides good models for the study of heterosis and species *** mapping serves as a chromosomal-level framework to investigate the molecular mechanisms of hybridization and *** this study,high-resolution linkage maps were constructed for the bay and Peruvian scallops with an interspecific hybrid *** linkage map of the bay scallop covered over 98.9% of the whole genome with 2994 mapped markers and the average marker interval of 0.32 *** the Peruvian scallop,1585 markers were mapped with the average maker interval of 0.51 cM,covering 97.7% of the *** the two linkage maps have 16 linkage groups,corresponding to the haploid chromosome number of the two ***,54.5% of markers exhibited significant deviation from the expected Mendelian ratio of segregation,lending in sights into the intrinsic incompatibility between the two *** related to growth and shell coloration were detected,which could explain 13.1%and 74.9% of the phenotypic variance,*** represents important information for further *** findings are an important addition to the genomic resources for scallop genetic studies,and are especially useful for investigations on genomic incompatibility for hybridization,genome evolution of closely related species,and genetic enhancement programs in aquaculture.

关键词： Scallop Genetic map 2b-RAD genotyping Quantitative trait locus mapping interspecific hybridization