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Science China Chemistry 2025年第1期68卷 297-307页

作者： Bin Fang Limin Wang Haoqin Li Jiaxin Zhang Yang Ding Pan Pan Li Bo Peng Hua Bai Lin Li Frontiers Science Center for Flexible Electronics Xi'an Institute of Flexible Electronics (IFE) and Xi'an Institute of Biomedical Materials &Engineering Northwestern Polytechnical University Institute of Flexible Electronics (IFE Future Technologies) Xiamen University

Hepatic ischemia-reperfusion injury（HIRI） is characterized by mitochondrial dysfunction and oxidative stress. Monitoring mitochondrial hydrogen peroxide（mtH2O2） levels in real-time through super-resolution imaging is crucial for elucidating its distribution in live cells and its mechanism of action during HIRI. However, low-background fluorogenic probes have been overlooked in the context of super-resolution imaging. In this study, we developed a low-background fluorogenic probe（MitoWG） with the potential for super-resolution morphology-correlated mitochondrial identification to track the fluctuates of mtH2O2in HIRI. Activation of the desirable fluorescence properties of the probe by mtH2O2was confirmed using structural illumination microscopy（SIM）, enabling high-quality mitochondrial imaging with exceptional specificity and *** in mtH2O2levels were successfully observed in both cellular and rat models of HIRI. Furthermore, we associated the decline in mitochondrial redox homeostasis with accelerated mtH2O2production during HIRI, which triggered mitophagy deficiency and led to cell death. In conclusion, Mito-WG possesses excellent photophysical and low-background properties for SIM imaging, making it a promising tool for mtH2O2tracking in HIRI research and clinical diagnosis.

关键词： hepatic ischemia-reperfusion injury low-background mtH2O2-activatable super-resolution imaging fluorogenic probe

Two-photon dual-channel fluorogenic probe for in situ imaging the mitochondrial H_(2)S/viscosity in the brain of drosophila Parkinson’s disease model

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Chinese Chemical Letters 2020年第11期31卷 2903-2908页

作者： Zhijie Fang Zhe Su Wenjing Qin Hao Li Bing Fang Wei Du Qiong Wu Bo Peng Peng Li Haidong Yu Lin Li Wei Huang Key Laboratory of Flexible Electronics(KLOFE)&Institute of Advanced Materials(1AM) Nanjing Tech University(NanjingTech)Nanjing 211816China Shaanxi Institute of Flexible Electronics(SIFE)&Institute of Biomedical Materials&Engineering(IBME) Northwestern Polytechnical University(NPU)Xi'an 710072China

H2S is an essential gas signal molecule in cells,and viscosity is a key internal environmental *** studies have shown that H_(2)S acts as a cytoarchitecture agent and gas transmitter in many tissues,e.g.,as a regulator of neuroendocrine in the brain for mediating vascular tone in blood *** viscosity is an important parameter for judging whether mitochondrial function is *** has been reported that oxidative stress and mitochondrial dysfunction are connected with Parkinson’s disease(PD),and the protective role of H_(2)S in PD models has been extensively ***,Mito-HS,a new two-photon fluorescent probe was demonstrated to detect cross-talk between the two channels of mitochondrial viscosity and H_(2)S ***,this probe could detect the relative amount of and changes in mitochondrial H2S in situ due to the reduced mitochondrial targeting ability after reaction with H_(2)*** results show that H2S in mitochondria is inversely related to *** PD model has a lower H2S in mitochondria and a higher mitochondrial viscosity than did the *** result is important for our deep understanding of PD and its causes.

关键词： Two-photon H_(2)S Viscosity fluorogenic probe Parkinson’s disease

Fluorescence imaging mitochondrial copper(Ⅱ) via photocontrollable fluorogenic probe in live cells

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Chinese Chemical Letters 2017年第10期28卷 1965-1968页

作者： Liulin Wang Buxiang Chen Pingping Peng Wenbo Hu Zhipeng Liu Xiaohua Pei Weihong Zhao Chengwu Zhang Lin Li Wei Huang Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM) Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM) Nanjing Tech University (Nanjing Tech) Division of Nephrology Department of Geriatrics The First Affiliated Hospital of Nanjing Medical University Key Laboratory for Organic Electronics and Information Displays & Institute of Advanced Materials (IAM) Nanjing University of Posts &Telecommunications

Monitoring mitochondrial derived copper（Ⅱ） in live cells is highly demanded, but accurately detecting is unmet due to the interference with cytoplasmic copper（Ⅱ）. Herein, we have reported the design,synthesis and characterization of photocontrollable fluorogenic probe, MCu-3, which is equipped with a photo-labile group（nitrobenzyl group） and mitochondria targeting unit（triphenylphosphonium salt）.This novel probe showed an intense fluorescence enhancement in response to copper（Ⅱ） without interference from other metal cations in the biological condition（p H 6–9）. The detection limit is 1.7 ×10^（-7） mol/L in HEPES buffer. The confocal fluorescence imaging results demonstrated MCu-3 can visualize mitochondrial copper（Ⅱ） in live mammalian cells. The clear advantage of our photocontrollable method is successful to avoid the influence of cytoplasmic copper（Ⅱ） during mitochondria specific detection.

关键词： Mitochondria Photocontrollable fluorogenic probe Fluorescence imaging Copper(Ⅱ)

A mitochondria-targeted H_(2)S-activatable fluorogenic probe for tracking hepatic ischemia-reperfusion injury

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Chinese Chemical Letters 2024年第6期35卷 370-375页

作者： Bin Fang Jiaqi Yang Limin Wang Haoqin Li Jiaying Guo Jiaxin Zhang Qingyuan Guo Bo Peng Kedi Liu Miaomiao Xi Hua Bai Li Fu Lin Li School of Materials Science and Engineering Northwestern Polytechnical UniversityXi’an 710072China Frontiers Science Center for Flexible Electronics Xi’an Institute of Flexible Electronics(IFE)and Xi’an Institute of Biomedical Materials&EngineeringNorthwestern Polytechnical UniversityXi’an 710072China Key laboratory of Flexible Electronics of Zhejiang Province Ningbo Institute of Northwestern Polytechnical UniversityNingbo 315103China The Institute of Flexible Electronics(IFE Future Technologies)Xiamen UniversityXiamen 361005China Wuhan National Laboratory for Optoelectronics-Advanced Biomedical Imaging Facility Huazhong University of Science and TechnologyWuhan 430074China TANK Medicinal Biology Institute of Xi’an Xi’an 710032China

Hepatic ischemia-reperfusion injury(HIRI)is the cause of postoperative hepatic dysfunction and failure,and even *** an important biological effector molecule,hydrogen sulfide(H_(2)S)of mitochondria as a gasotransmitter that is usually used to protect against acute HIRI ***,the exact relationship between HIRI and mitochondrial H_(2)S remains tangled due to the lack of an effective analytical ***,we have fabricated a mitochondria-targeted H_(2)S-activatable fluorogenic probe(Mito-GW)to explore the stability of mitochondrial H_(2)S and track the changes of mitochondrial H_(2)S during the *** virtue of pyridinium electropositivity and its amphiphilicity,Mito-GW could accumulate in *** goes through an analyte-prompted immolation when reacts with H_(2)S,resulting in the releasing of the fluorophore(GW).Therefore,the extent of Mito-GW conversion to GW can be used to evaluate the changes of mitochondrial H_(2)S level in living cells and *** proof-of-principle,we have used MitoGW to demonstrate the mitochondria H_(2)S-levels increase and then decrease during HIRI in vitro and in *** research highlights the tremendous potential of Mito-GW as a mitochondrial H_(2)S fluorogenic probe in elucidating the pathogenesis of HIRI,providing a powerful tool for promoting future research on hepatology.

关键词： Mitochondria-targeted Bioimaging H2S-activatable fluorogenic probe Hepatic ischemia-reperfusion injury(HIRI)

A fluorogenic probe for SNAP-tag protein based on ESPT ratiometric signals

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Chinese Chemical Letters 2023年第5期34卷 641-644页

作者： Jin Li Qinglong Qiao Yiyan Ruan Ning Xu Wei Zhou Guixin Zhang Jingli Yuan Zhaochao Xu State Key Laboratory of Fine Chemicals School of ChemistryDalian University of TechnologyDalian 116024China CAS Key Laboratory of Separation Science for Analytical Chemistry Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China University of Chinese Academy of Sciences Beijing 100049China General Surgery Department The First Affiliated Hospital of Dalian Medical UniversityDalian 116011China

Protein self-labeling tags achieve selective fusion and labeling of target proteins through genetic coding technology,but require exogenous fluorescent probes with fluorogenicity for protein tag binding to have the performance of wash-free fluorescence imaging in live *** this paper,we reported a fluorogenic probe 1 capable of ratiometric fluorescence recognition of SNAP-tag *** this probe,the O6-benzylguanine derivative of 3-hydroxy-1,8-naphthalimide underwent a selective covalent linkage reaction with SNAP-tag *** hydroxyl group on the naphthalimide fluorophore formed a hydrogen bond with the functional group near the protein *** excited state proton transfer occurred after illumination,to obtain the ratio fluorescence signal from blue emission to red emission,realizing the wash-free fluorescence imaging of the target proteins.

关键词： fluorogenic probe Ratiometric ESPT SNAP-tag Wash-free imaging

A novel fluorogenic probe for monoamine oxidase assays

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Chinese Chemical Letters 2008年第8期19卷 947-950页

作者： You You Lu Yu Guang Wang Bin Dai Yi Qi Dai Zhao Wang Zheng Wei Fu Qing Zhu College of Biological and Environmental Engineering Zhejiang University of Technology Hangzhou 310014 China

Monoamine oxidase is flavoenzymes, widely distributed in mammals. It is well recognized that MAOs serve an important role in metabolism that they have close relationship with health .Along with the discoveries between MAOs and neurotic disease, more and more studies have been jumped in .In this paper, we design a new probe for assaying the activities of MAOs. The results showed that the probe [7-（3-aminopropoxy）coumarin] is simple, effective and sensitive for MAOB.

关键词： Monoamine oxidase fluorogenic probe Assay

A Non-Peptide-Based fluorogenic Small-Molecule probe for Elastase and Its Application

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A Non-Peptide-Based Fluorogenic Small-Molecule Probe for Ela...

High-contrast and real-time visualization of membrane proteins in live cells with malachite green-based fluorogenic probes

第八届全国化学生物学学术会议

作者： Qi Sun Jun Li Wan-Nian Liu Qing-Jian Dong Wen-Chao Yang Guang-Fu Yang Key Laboratory of Pesticide ＆ Chemical Biology College of ChemistryMinistry of EducationCentral China Normal University

＜正＞Human neutrophil elastase（HNE） has been identified as a potential therapeutic target for the discovery of anti-inflammatory drugs for ***,the HNE-targeting drug discovery has achieved little progres s due to the unavailability of synthetic substrates for measuring the activity of ***,a small-molecule compound,2,2,3,3,3-pentafluoro-N-（2-oxo-4-（trifluoromethyl）-2H-chromen-7-yl）- propanamide（compound 4）,has been successfully designed as the first-ever

关键词： Human neutrophil elastase fluorogenic probe inhibitor virtual screening

来源： cnki会议评论

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Chinese Chemical Letters 2022年第3期33卷 1637-1642页

作者： Yefeng Chen Chenghong Xue Jie Wang Minqiu Xu Yuyao Li Yiru Ding Heng Song Weipan Xu Hexin Xie State Key Laboratory of Bioreactor Engineering Shanghai Key Laboratory of New Drug DesignFrontiers Science Center for Materiobiology and Dynamic ChemistrySchool of PharmacyEast China University of Science and TechnologyShanghai 200237China

Imaging dynamics of membrane proteins of live cells in a wash-free and real-time manner has been a challenging task. Herein, we report unprecedented applications of malachite green(MG), an organic dye widely used in pigment industry, as a switchable fluorophore to monitor membrane enzymes or noncatalytic proteins in live cells. Conformationally flexible MG is non-fluorescent in aqueous solution, yet covalent binding with endogenous proteins of cells significantly enhances its fluorescence at 670 nm by restricting flexibility of dye. Integrating a phosphate-caged quinone methide precursor with MG yielded a covalent labeling fluorogenic probe, allowing real-time imaging of membrane alkaline phosphatase(ALP,a model catalytic protein) activity in live cells with over 100-fold enhancement of fluorescence ***, MG is also applicable to image non-catalytic protein by conjugation with protein-specific ligand. A fluorogenic probe consisted of c-RGDf K peptide and MG proved to be compatible with wash-free and real-time visualization of non-catalytic integrin α_(v)β_(3) in live cells with high contrast.

关键词： Cellular imaging fluorogenic probe Environment-sensitive fluorophore Alkaline phosphatase Integrin

Detection of Hepatitis B Virus DNA by Duplex Scorpion Primer-based PCR Assay

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Chinese Journal of Chemistry 2004年第9期22卷 903-907页

作者：孔德明沈含熙宓怀风 State Key Laboratory of Functional Polymer Materials for Adsorption and Separation Chemical School Nankai University Tianjin 300071 China b Chemical School Nankai University Tianjin 300071 China Chemical School Nankai University Tianjin 300071China State Key Laboratory of Functional Polymer Materials for Adsorption and Separation Chemical SchoolNankai University Tianjin 300071 China

The application of a new fluorogenic probe-based PCR assay (PCR duplex scorpion primer assay) to the detection of Hepatitis B virus (HBV) DNA in human sera was described. Duplex scorpion primer is a modified variant of duplex Amplifluor, and the incorporation of a PCR stopper between probe and primer sequences improve the detection specificity and sensitivity. Combined with PCR amplification, this probe can give unambiguous positive results for the reactions initiated with more than 20 HBV molecules. In addition, the particular unimolecular probing mechanism of this probe makes the use of short target-specific probe sequence possible, which will render this probe applicable in some specific systems.

关键词： duplex scorpion primer hepatitis B virus (HBV) DNA fluorogenic probe