A mitochondria-targeted H_(2)S-activatable fluorogenic probe for tracking hepatic ischemia-reperfusion injury

作     者：Bin Fang Jiaqi Yang Limin Wang Haoqin Li Jiaying Guo Jiaxin Zhang Qingyuan Guo Bo Peng Kedi Liu Miaomiao Xi Hua Bai Li Fu Lin Li 

作者机构：School of Materials Science and EngineeringNorthwestern Polytechnical UniversityXi’an 710072China Frontiers Science Center for Flexible ElectronicsXi’an Institute of Flexible Electronics(IFE)and Xi’an Institute of Biomedical Materials&EngineeringNorthwestern Polytechnical UniversityXi’an 710072China Key laboratory of Flexible Electronics of Zhejiang ProvinceNingbo Institute of Northwestern Polytechnical UniversityNingbo 315103China The Institute of Flexible Electronics(IFEFuture Technologies)Xiamen UniversityXiamen 361005China Wuhan National Laboratory for Optoelectronics-Advanced Biomedical Imaging FacilityHuazhong University of Science and TechnologyWuhan 430074China TANK Medicinal Biology Institute of Xi’anXi’an 710032China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2024年第35卷第6期

页      面：370-375页

核心收录：

学科分类：081704[工学-应用化学] 1002[医学-临床医学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 0703[理学-化学] 10[医学] 

基　　金：financially supported by the National Natural Science Foundation of China(Nos.22077101,22004099) the Joint Research Funds of Department of Science&Technology of Shaanxi Province and Northwestern Polytechnical University(Nos.2020GXLH-Z-008,2020GXLH-Z-021,2020GXLH-Z-023) Natural Science Foundation of Shaanxi Province(No.2022JM-130) The Natural Science Foundation of Ningbo(Nos.202003N4049,202003N4065) the Open Project Program of Wuhan National Laboratory for Optoelectronics(Nos.2020WNLOKF023,2022WNLOKF009) Innovation Foundation for Doctor Dissertation of Northwestern Polytechnical University(No.CX2022034) Innovation Capability Support Program of Shaanxi(No.2023-CX-PT-23) 

主　　题：Mitochondria-targeted Bioimaging H2S-activatable Fluorogenic probe Hepatic ischemia-reperfusion injury(HIRI) 

摘      要：Hepatic ischemia-reperfusion injury(HIRI)is the cause of postoperative hepatic dysfunction and failure,and even *** an important biological effector molecule,hydrogen sulfide(H_(2)S)of mitochondria as a gasotransmitter that is usually used to protect against acute HIRI ***,the exact relationship between HIRI and mitochondrial H_(2)S remains tangled due to the lack of an effective analytical ***,we have fabricated a mitochondria-targeted H_(2)S-activatable fluorogenic probe(Mito-GW)to explore the stability of mitochondrial H_(2)S and track the changes of mitochondrial H_(2)S during the *** virtue of pyridinium electropositivity and its amphiphilicity,Mito-GW could accumulate in *** goes through an analyte-prompted immolation when reacts with H_(2)S,resulting in the releasing of the fluorophore(GW).Therefore,the extent of Mito-GW conversion to GW can be used to evaluate the changes of mitochondrial H_(2)S level in living cells and *** proof-of-principle,we have used MitoGW to demonstrate the mitochondria H_(2)S-levels increase and then decrease during HIRI in vitro and in *** research highlights the tremendous potential of Mito-GW as a mitochondrial H_(2)S fluorogenic probe in elucidating the pathogenesis of HIRI,providing a powerful tool for promoting future research on hepatology.