Deciphering the role of mtH2O2 in hepatic ischemia-reperfusion injury mechanisms with low-background super-resolution fluorogenic probe
作者机构：Frontiers Science Center for Flexible Electronics Xi'an Institute of Flexible Electronics (IFE) and Xi'an Institute of Biomedical Materials &Engineering Northwestern Polytechnical University Institute of Flexible Electronics (IFE Future Technologies) Xiamen University
出 版 物：《Science China Chemistry》 (中国科学:化学(英文版))
年 卷 期：2025年第68卷第1期
页 面：297-307页
核心收录：
学科分类：1002[医学-临床医学] 081704[工学-应用化学] 07[理学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 10[医学]
基 金：supported by the National Natural Science Foundation of China (62288102, 22077101, 62475216) the Natural Science Foundation of Shaanxi Province (2022JM-130) the Key Research and Development Program of Shaanxi (2024GH-ZDXM-37) the Innovation Capability Support Program of Shaanxi (2023-CX-PT-23) the Natural Science Basic Research Program of Shaanxi (2024JC-YBQN-0919) the Open Project Program of the Analytical&Testing Center of Northwestern Polytechnical University (2020T018) the Postdoctoral Fellowship Program of China Postdoctoral Science Foundation (CPSF)(GZC20240889) the Innovation Foundation for Doctor Dissertation of Northwestern Polytechnical University (CX2022034, CX2023098) the Startup Program of XMU (L.L.) Fundamental Research Funds for the Central Universities
主 题：hepatic ischemia-reperfusion injury low-background mtH2O2-activatable super-resolution imaging fluorogenic probe
摘 要：Hepatic ischemia-reperfusion injury（HIRI） is characterized by mitochondrial dysfunction and oxidative stress. Monitoring mitochondrial hydrogen peroxide（mtH2O2） levels in real-time through super-resolution imaging is crucial for elucidating its distribution in live cells and its mechanism of action during HIRI. However, low-background fluorogenic probes have been overlooked in the context of super-resolution imaging. In this study, we developed a low-background fluorogenic probe（MitoWG） with the potential for super-resolution morphology-correlated mitochondrial identification to track the fluctuates of mtH2O2in HIRI. Activation of the desirable fluorescence properties of the probe by mtH2O2was confirmed using structural illumination microscopy（SIM）, enabling high-quality mitochondrial imaging with exceptional specificity and *** in mtH2O2levels were successfully observed in both cellular and rat models of HIRI. Furthermore, we associated the decline in mitochondrial redox homeostasis with accelerated mtH2O2production during HIRI, which triggered mitophagy deficiency and led to cell death. In conclusion, Mito-WG possesses excellent photophysical and low-background properties for SIM imaging, making it a promising tool for mtH2O2tracking in HIRI research and clinical diagnosis.
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