Fluorescence imaging mitochondrial copper(Ⅱ) via photocontrollable fluorogenic probe in live cells

作     者：Liulin Wang Buxiang Chen Pingping Peng Wenbo Hu Zhipeng Liu Xiaohua Pei Weihong Zhao Chengwu Zhang Lin Li Wei Huang 

作者机构：Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM) Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM) Nanjing Tech University (Nanjing Tech) Division of Nephrology Department of Geriatrics The First Affiliated Hospital of Nanjing Medical University Key Laboratory for Organic Electronics and Information Displays & Institute of Advanced Materials (IAM) Nanjing University of Posts &Telecommunications 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2017年第28卷第10期

页      面：1965-1968页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 

基　　金：supported by the National Natural Science Foundation of China (Nos. 81672508, 61505076) Natural Science Foundation of Jiangsu Province (No. BK20140951) Key University Science Research Project of Jiangsu Province (No. 16KJA180004) SICAM Fellowship & Scholarship by Jiangsu National Synergetic Innovation Center for Advanced Materials 

主　　题：Mitochondria Photocontrollable Fluorogenic probe Fluorescence imaging Copper(Ⅱ) 

摘      要：Monitoring mitochondrial derived copper（Ⅱ） in live cells is highly demanded, but accurately detecting is unmet due to the interference with cytoplasmic copper（Ⅱ）. Herein, we have reported the design,synthesis and characterization of photocontrollable fluorogenic probe, MCu-3, which is equipped with a photo-labile group（nitrobenzyl group） and mitochondria targeting unit（triphenylphosphonium salt）.This novel probe showed an intense fluorescence enhancement in response to copper（Ⅱ） without interference from other metal cations in the biological condition（p H 6–9）. The detection limit is 1.7 ×10^（-7） mol/L in HEPES buffer. The confocal fluorescence imaging results demonstrated MCu-3 can visualize mitochondrial copper（Ⅱ） in live mammalian cells. The clear advantage of our photocontrollable method is successful to avoid the influence of cytoplasmic copper（Ⅱ） during mitochondria specific detection.