检索结果-南通市图书馆

Effect of Conjugated Linoleic Acid on tumor cell Growth and Angiogenesis

在线全文

学校读者我要写书评

暂无评论

Effect of Conjugated Linoleic Acid on Tumor Cell Growth and ...

Observation of ultra-fine Surface structure of tumor cell in Synchronization with atomic force microscopy

“基因、进化与生理功能多样性”海内外学术研讨会暨中国生理学会第七届比较生理学学术会议

作者： David Kerr Department of Clinical Pharmacology University of OxfordOxford UK

A dietary fatty acid,cis-9,trans- 11-conjugated linoleic acid(CLA) derived from dairy products and meats of ruminant animal, has been shown to be a potent antitumour agent in vitro and in *** is capable of inducing tumour cell apoptosis by up-regulation of p53,p21 and Bax in some human cancer cell ***,its precise mechanism in anti-tumour progression remains elusive. In this study,we found that a high dose of CLA(144μM) but not a low dose(36μM) decreased Akt and eNOS phosphorylation in GP2D(a human colon cancer cell line) after vascular endothelial growth factor(VEGF-50 ng/mL) *** also demonstrated that CLA at the high dose completely inhibits VEGF-induced human umbilical vein endothelial cell(HUVEC) capillary-like tube formation after 7 days co-culture with murine fibroblast *** conclusion,the CLA might play an important role on tumer cell growth and angiogenesis by inhibiting the eNOS and AKT phosphorylation in relation to nitric oxide (NO) ***,we are defining a direct effect of CLA on tumour proliferation, apoptosis and migration as well as its significant biological function in different tumour cell lines.

关键词： Cojugated linoleic acid Endothelial nitric oxide synthase tumor cell Co-culture Angiogenesis

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Observation of ultra-fine Surface structure of tumor cell in...

Mitochondrial-derived ROS in edelfosine-induced apoptosis in yeasts and tumor cells

第十次中国生物物理学术大会

作者：朱杰孙润广齐浩陈文芳 College of Life Science Northwest Sci＆Tech University of Agriculture and Forestry Biophysics Lab College of Physics and InformationShaanxi Normal University College of Life Science Shaanxi Normal University College of Life Science Shaanxi Normal University

＜正＞Introduction: Multiple pathways are involved in maintaining the genetic integrity of a cell after its exposure to physical environments. Although repair mechanisms such as homologous recombination and non-homologous end-joining are important mammalian responses to double-strand DNA damage, cell cycle regulation is perhaps the most important determinant of physical environments sensitivity. A common cellular response to DNA-damaging agents is the activation of cell cycle checkpoints. The DNA

关键词： Atomic force microscope Synchronization tumor cell Ultrafine Structure cell Cycle

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2007年第6期28卷 888-894页

作者： Consuelo GAJATE Faustino MOLLINEDO Cancer Research Center Institute of Molecular and CellularBiology of CancerSpanish National Research Council-University of SalamancaSalamanca 37007Spain Research UnitUniversityHospital of SalamancaSalamanca 37007Spain Cancer Research Center Institute of Molecular and CellularBiology of CancerSpanish National Research Council-University of SalamancaSalamanca 37007Spain

Aim:To investigate whether a similar process mediates cytotoxicity of 1-O-octadecyl-2-O-methyl-rac-glycero-3-phosphocholine （ET-18-OCH3,edelfosine） inboth yeasts and human tumor ***:A modified version of a previouslydescribed assay for the intracellular conversion of nitro blue tetrazolium to formazanby superoxide anion was used to measure the generation of reactive oxygen spe-cies （ROS）.Apoptotic yeast cells were detected using terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling （TUNEL） *** fragmenta-tion and the generation of ROS were measured by cytofluorimetric analysis inJurkat ***:Edelfosine induced apoptosis in Saccharomyces cerevisiae,as assessed by TUNEL ***,edelfosine induced a time-and con-centration-dependent generation of ROS in ***,an inhibitor of themitochondrial electron transport chain,prevented ROS generation and apoptosisin response to edelfosine in S cerevisiae.α-Tocopherol abrogated the edelfosine-induced generation of intracellular ROS and *** also inducedan increase of ROS in human leukemic cells that preceded *** of Bcl-2 by gene transfer abrogated both ROS generation andapoptosis induced by edelfosine in leukemic *** in the relative mito-chondrial membrane potential were detected in both yeasts and Jurkat ***:These results indicate that edelfosine induces apoptosis in yeasts inaddition to human tumor cells,and this apoptotic process involves mitochondria,likely through mitochondrial-derived *** data also suggest that yeastscan be used as a suitable cell model in elucidating the antitumor mechanism ofaction of edelfosine.

关键词： edelfosine alkyl-lysophospholipid analog antitumor drug apoptosis reactive oxygen species mitochondria Bcl-2 yeast tumor cell

Antisense-Induced Blockade of GATA-3 Expression Could Inhibit Th2 Excursion of tumor cells in vitro and in vivo

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

cellular & Molecular Immunology 2005年第3期2卷 189-196页

作者： Dongzhu Yao~1 Xiaojun Zhang~1 Haiming Wei~1 Zhigang Tian~(1,2) ~1Institute of Immunology,School of Life Sciences,University of Science & Technology of China ~2Corresponding to:Dr.Zhigang Tian,School of Life Sciences,University of Science & Technology of China,No.443,Huangshan Road,Hefei 230027,China. Institute of Immunology School of Life Sciences University of Science and Technology of China Hefei 230027 China.

Previous studies have shown that tumor cells predominantly express Th2 type cytokines and transcription factors. GATA-3, as a Th2-specific transcription factor, plays a central role in positive-regulating Th2 development. So whether the expression of GATA-3 in tumor cells has any effect on tumor development is a question of interest. In the present study, we inhibited the expression of GATA-3 in tumor cells through antisense RNA blockade technique, and observed its effects on tumor in vitro and in vivo. Our results showed that antisense GATA-3 treatment could inhibit the expression of TNF-α and Th2 cytokines in tumor cells, and antisense-induced blockade of GATA-3 could also depress tumor growth in tumor-bearing mice. We suggest that the ratio of T-bet/GATA-3 can be evaluated as a more important marker of the status of Thl/Th2 type. And our results might provide some evidence about the molecular regulatory mechanisms in tumor cell development. cellular ＆ Molecular Immunology. 2005 ;2（3 ）： 189-196.

关键词： GATA-3 antisense RNA tumor cell Th1/Th2 cytokine and transcription factor

Cytotoxic Effects of Biodegradation of Pure Mg and MAO-Mg on tumor cells of MG63 and KB

在线全文

学校读者我要写书评

暂无评论

Journal of Materials Science & Technology 2014年第5期30卷 487-492页

作者： Qiang Wang1a Shi Jin Xiao Lin Yang Zhang Ling Ren Ke Yang School of Stomatology China Medical University Institute of Metal Research Chinese Academy of Sciences

Magnesium （Mg） based metals possess unique features of biodegradation that can be used for different purposes. In this study, two tumor cells, MG63 and KB, were cultured directly on a pure Mg with and without MAO coating to evaluate their cytotoxic effects on the tumor cells. It was found that both MG63 cells and KB cells adhered better on the surface of the coated Mg samples than those on the pure Mg samples. However, the survivals of two kinds of tumor cells were obviously inhibited on both Mg samples with and without coating, compared with that on the pure titanium. In addition, the survival of MG63 cells was more susceptive to the Mg degradation compared with the KB cells. Thus, the application of such effect should take account of that the degradation of Mg based metal may result in different cytotoxic effect on different cells.

关键词： Magnesium ahoy Degradation Cytotoxic effect tumor cell

Inhibitory effect of agmatine on proliferation of tumor cells by modulation of polyamine metabolism

在线全文

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2005年第5期26卷 616-622页

作者： Ji-fang WANG~2,Rui-bin SU~2,Ning WU,Bo XU,Xin-qiang LU,Yin LIU,Jin LI~3Beijing Institute of Pharmacology and Toxicology,Beijing 100850,China Beijing Institute of Pharmacology and Toxicology Beijing China

Aim:To assess the inhibitory effect of agmatine on tumor growth in vivo andtumor cell proliferation in ***:The transplanted animal model,[~3H]thymidine incorporation assay,3-[4,5-dimethythiazol-2-yl]-2,5-diphenyltetrazo-lium assay,and lactate dehydrogenase(LDH)release assay were ***:Agmatine,at doses of 5-40 mg/kg,suppressed the S180sarcoma tumorgrowth dose-dependently in mice in vivo and the highest inhibitory ratio reached31.3% in Kunming mice and 50.0% in Balb/c mice,*** resultswere obtained in the transplanted B16melanoma tumor ***(1-1000 μmol/L)was able to attenuate the proliferation of cultured MCF-7 humanbreast cancer cells in vitro in a concentration-dependent manner and the highestinhibitory ratio reached 50.3% in the[~3H]thymidine incorporation ***,in the LDH release assay,spermine(20μmol/L)and spermidine(20μmol/L)increased the LDH release significantly,but agmatine(1-1000μmol/L)did not,indicating that the inhibitory effect of agmatine on the proliferation ofMCF was not related to cellular *** the[~3H]thymidine incorporation assay,putrescine(12.5-100.0μmol/L)could reverse the inhibitory effect of agmatine onthe proliferation of MCF concentration-dependently,suggesting that the inhibi-tory effect of agmatine on the proliferation of MCF might be associated with adecreased level of the intracellular polyamines ***:Agmatine hadsignificant inhibitory effect on transplanted tumor growth in vivo and prolifera-tion of tumor cells in vitro,and the mechanism might be a result of inducingdecrease of intracellular polyamine contents.

关键词： agmatine polyamine tumor cell proliferation

From small to large-The application of in situ polymerization within tumor cells

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Aggregate

Aggregate 2024年第4期5卷 33-53页

作者： Mingjie Jia Yonghang Liu Peng Wei Tao Yi State Key Laboratory for Modification of Chemical Fibers and Polymer Materials Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative MedicineCollege of Chemistry and Chemical EngineeringDonghua UniversityShanghaiChina School of Pharmacy and State Key Laboratory of Quality Research in Chinese Medicine Macao University of Science and TechnologyMacaoChina

The“laboratory”of cells has the capacity to polymerize monosaccharides,amino acids,and *** cells,characterized by the overexpression of multi-ple enzymes and existing in a slightly acidic and highly redox-potent environment,have attracted the attention of chemists aiming to transfer chemical reactions from the traditional laboratoryflask to this“cellular laboratory”.Polymers,resulting from the repetitive linkage of monomers,have garnered extensive utility in the biomed-icalfield due to their diverse structural and physicochemical *** the polymerization reaction proceeds in situ within the tumor cells,this in situ trans-formation from small-to-large combines the rapid uptake of monomeric molecules with the strong retention ability of polymers,exerting a profound impact on drug delivery within ***,it shows promising applications in the regulation of cell behavior,imaging,therapy,and *** the diverse functions of in situ polymerization in relation to tumor cells,this review focuses on a comprehen-sive examination of various strategies for in situ polymerization within tumor cells,categorizing these strategies based on the formation mechanisms of *** applications in this domain concerning in situ polymerization within tumor cells are also ***,a discussion of specific limitations in current research and insights into potential future directions from the authors’perspective are provided.

关键词： in situ polymerization tumor cell

Dendritic cell-Derived Exosomes Stimulate Stronger CD8^+ CTL Responses and Antitumor Immunity than TVimor cell-Derived Exosomes

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

cellular & Molecular Immunology 2006年第3期3卷 205-211页

作者： Siguo Hao Ou Bai Jinying Yuan Mabood Qureshi Jim Xiang Research Unit Division of Health Research Saskatchewan Cancer Agency Departments of Oncology Immunology and Pathology College of Medicine University of Saskatchewan Saskatoon Saskatchewan STN 4H4 Canada Siguo Hao and Ou Bai made the same contribution in this study

Exosomes （EXO） derived from dendritic cells （DC） and tumor cells have been used to stimulate antitumor immune responses in animal models and in clinical trials. However, there has been no side-by-side comparison of the stimulatory efficiency of the antitumor immune responses induced by these two commonly used EXO vaccines. In this study, we selected to study the phenotype characteristics of EXO derived from a transfected EG7 tumor cells expressing ovalbumin （OVA） and OVA-pulsed DC by flow cytometry. We compared the stimulatory effect in induction of OVA-specific immune responses between these two types of EXO. We found that OVA protein-pulsed DCOVA-derived EXO （EXODC） can more efficiently stimulate naive OVA-specific CD8^＋ T cell proliferation and differentiation into cytotoxic T lymphocytes in vivo, and induce more efficient antitumor immunity than EG7 tumor cell-derived EXO （EXOEG7）. In addition, we elucidated the important role of the host DC in EXO vaccines that the stimulatory effect of EXO is delivered to T cell responses by the host DC. Therefore, DC-derived EXO may represent a more effective EXO-based vaccine in induction of antitumor immunity. cellular ＆ Molecular Immunology. 2006;3（3）：205-211.

关键词： dendritic cell exosome tumor cell cytotoxic T lymphocyte antitumor immunity

Functional significance of erythropoietin receptor on tumor cells

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2006年第46期12卷 7460-7462页

作者： Kodetthoor B Udupa Reynolds Institute on Aging Department of Geriatrics Physiology and Biophysics University of Arkansas for Medical Sciences and Central Arkansas Veterans Healthcare System 4300 West Seventh Street Little Rock AR United States

Erythropoietin (Epo) is the regulator of red blood cell formation. Its receptor (EpoR) is now found in many cells and tissues of the body. EpoR is also shown to occur in tumor cells and Epo enhances the proliferation of these cells through cell signaling. EpoR antagonist can reduce the growth of the tumor in vivo. In view of our current knowledge of Epo, its recombinant forms and receptor, use of Epo in cancer patients to enhance the recovery of hematocrit after chemotherapy treatment has to be carefully evaluated.

关键词： Erythropoietin tumor cell Receptor cell signaling Proliferation Erythroid cell