Antisense-Induced Blockade of GATA-3 Expression Could Inhibit Th2 Excursion of Tumor Cells in vitro and in vivo

作     者：Dongzhu Yao~1 Xiaojun Zhang~1 Haiming Wei~1 Zhigang Tian~(1,2) ~1Institute of Immunology,School of Life Sciences,University of Science & Technology of China ~2Corresponding to:Dr.Zhigang Tian,School of Life Sciences,University of Science & Technology of China,No.443,Huangshan Road,Hefei 230027,China. 

作者机构：Institute of Immunology School of Life Sciences University of Science and Technology of China Hefei 230027 China. 

出 版 物：《Cellular & Molecular Immunology》 (中国免疫学杂志（英文版）)

年 卷 期：2005年第2卷第3期

页      面：189-196页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：GATA-3 antisense RNA tumor cell Th1/Th2 cytokine and transcription factor 

摘      要：Previous studies have shown that tumor cells predominantly express Th2 type cytokines and transcription factors. GATA-3, as a Th2-specific transcription factor, plays a central role in positive-regulating Th2 development. So whether the expression of GATA-3 in tumor cells has any effect on tumor development is a question of interest. In the present study, we inhibited the expression of GATA-3 in tumor cells through antisense RNA blockade technique, and observed its effects on tumor in vitro and in vivo. Our results showed that antisense GATA-3 treatment could inhibit the expression of TNF-α and Th2 cytokines in tumor cells, and antisense-induced blockade of GATA-3 could also depress tumor growth in tumor-bearing mice. We suggest that the ratio of T-bet/GATA-3 can be evaluated as a more important marker of the status of Thl/Th2 type. And our results might provide some evidence about the molecular regulatory mechanisms in tumor cell development. Cellular ＆ Molecular Immunology. 2005 ;2（3 ）： 189-196.