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Protein-sparing effect of carbohydrate in diets for juvenile turbot scophthalmus maximus reared at different salinities

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Chinese Journal of Oceanology and Limnology 2015年第1期33卷 57-69页

作者：曾霖雷霁霖艾春香洪万树刘滨 College of Ocean and Earth Sciences Xiamen University National Technology System for Flatfish Culture Industry Chinese Ministry of Agriculture Qingdao Key Laboratory for Marine Fish Breeding and Biology Yellow Sea Fisheries Research InstituteChinese Academy of Fishery Sciences

The aim of the present study was to investigate the protein-sparing effect of carbohydrate in diets for juvenile turbot(scophthalmus maximus) reared at five salinities(12,18,24,30,and 36).The fish were fed three isocaloric and isolipidic diets for 60 *** results show that specific growth rate(SGR)and feed conversion efficiency(FCE) were higher in fish reared at salinities of 18 and 36,but lower at *** fed with diet C25P40(25%carbohydrate and 40%protein) had lower SGR and FCE values compared with those fed with the C5P52(5%carbohydrate and 52%protein) and C15P46(15%carbohydrate and 46%protein) diets;however,there was no statistical difference between diet C5P52 and *** and FCE values were unaffected by diet composition in fish reared at salinity *** lipogenic enzyme activities were higher in fish reared at 18 and 36,but lower at 12,while glucokinase(GK) activity was higher in fish reared at 12,and lower at 18 and *** starch enhanced GK activity while depressing lipogenic enzyme ***,lipogenic enzyme activity increased with increasing dietary starch in fish reared at *** is recommended that salinity should be maintained > 12 in the farming of juvenile *** addition,an increase in gelatinized starch from 5%to 15%could spare 6%dietary protein in fish reared at salinities of 18-30,while higher salinity(36) could improve dietary carbohydrate use and enhance the protein-sparing effect,which is linked with the induction of lipogenic capacities.

关键词： scophthalmus maximus carbohydrate salinity protein-sparing effect hepatic glycometabolism

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Isolation and characterization of 45 polymorphic microsatellite loci of turbot(scophthalmus maximus)and cross-species amplification

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Chinese Journal of Oceanology and Limnology 2011年第2期29卷 311-316页

作者：侯仕营马爱军王新安黄智慧薛宝贵杨志曲江波 Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesQingdao Key Laboratory for Marine Fish Breeding and Biotechnology Rushan Fisheries Technical Extension Station Yantian Tianyuan Aquatic Limited Corporation

Turbot （scophthalmus maximus） is a flatfish species commercially important for aquaculture. In this study, we generated a microsatellite-enriched genomic DNA library for scophthalmus rnaxirnus, and then isolated and characterized 45 microsateIIite loci by genotyping 30 individuals. The observed number of alleles ranged from 2 to 19 with an average of 6.24, while the effective number of alleles ranged from 1.30 to 11.11 with an average of 3.66. The expected heterozygosities varied from 0.235 to 0.9254 and polymorphic information content ranged from 0.204 4 to 0.903 3, with an average of 0.622. Twelve loci deviated significantly from Hardy-Weinberg equilibrium, and no significant linkage disequilibrium was observed between any pair of loci after Bonferroni correction. In cross-species amplification, five flatfish species （Paralichthys lethostigma, Verasper rnoseri, platichthys stellatus, Hippoglossoides dubius and Cynoglossus semilaevis） showed at least one polymorphic locus. These polymorphic microsatellite loci should prove useful for population analysis of turbot and other related species.

关键词： turbot scophthalmus maximus enriched library microsatellite

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Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot scophthalmus maximus responded to hypo-saline seawater

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Journal of Oceanology and Limnology 2020年第2期38卷 467-479页

作者： CUI Wenxiao MA Aijun HUANG Zhihui WANG Xin’an SUN Zhibin LIU Zhifeng ZHANG Wei YANG Jingkun ZHANG Jinsheng QU Jiangbo College of Fisheries and Life Science Shanghai Ocean UniversityMinistry of EducationShanghai 201306China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesShandong Key Laboratory of Marine Fisheries Biotechnology and Genetic BreedingQingdao Key Laboratory for Marine Fish Breeding and BiotechnologyQingdao 266071China Yantai Tianyuan Aquatic Limited Corporation Yantai 264003China

Turbot harbor a relatively remarkable ability to adapt to opposing osmotic challenges and are an excellent model species to study the physiological adaptations of flounder associated with osmoregulatory *** kidney transcriptome of turbot treated 24 h in water of hypo-salinity(salinity 5)and seawater(salinity 30)was sequenced and *** silico analysis indicated that all unigenes had significant hits in seven *** functional annotation analysis of the transcriptome showed that the immune system and biological processes associated with digestion,absorption,and metabolism played an important role in the osmoregulation of turbot in response to *** of biological processes associated with inorganic channels and transporters indicated that mineral absorption and bile secretion contributed to iono-osmoregulation resulting in cell volume regulation and cell phenotypic ***,we analyzed and predicted the mechanisms of canonical signaling *** processes involved in renin secretion,ECM-receptor interaction,adherens junction,and focal adhesion played an important role in the plasticity phenotype in hypo-stress,while the signal transduction network composed of the MAPK signaling pathway and PI3K-Akt signaling pathway with GABAergic synapse,worked in hypoosmoregulation signal transduction in the *** addition,analysis of the tissue specificity of targeted gene expression using qPCR during salinity stress was carried *** results showed that the kidney,gill,and spleen were vital regulating organs of osmotic pressure,and the osmoregulation pattern of euryhaline fish dif fered among species.

关键词： osmoregulation mechanisms scophthalmus maximus hypo-saline signal transduction cell volume regulation gene expression

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Pharmacokinetics and tissue behavior of enrofloxacin and its metabolite ciprofloxacin in turbot scophthalmus maximus at two water temperatures

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Chinese Journal of Oceanology and Limnology 2012年第4期30卷 644-653页

作者：梁俊平李健赵法箴刘萍常志强 Fisheries College Ocean University of China Key Laboratory of Sustainable Development of Marine Fisheries Ministry of AgricultureYellow Sea Fisheries Research InstituteChinese Academy of Fishery Sciences

Turbot scophthalmus maximus, an important aquaculture species in China, currently suffers from epizootic diseases because of high density aquaculture. Enrofloxacin has been used to treat various systemic bacterial fish infections. However, studies concerning the pharmacokinetics of enrofloxacin in turbot are limited. In this study, the pharmacokinetics of enrofloxacin and its metabolite ciprofloxacin, were investigated in the turbot following intravenous and oral administration at 10 mg enrofloxacin/kg body weight, at 16℃ and 10℃ water temperatures. The concentrations of enrofloxacin and ciprofloxacin in the main tissues （plasma, muscle, liver and kidney） were detected by HPLC. The results show that the plasma concentration-time data for enrofloxacin were best described as a two-compartment open model after intravenous and oral administration. Three pharmacokinetic equations were established between the concentrations and temperatures. The kinetic profile of enrofloxacin was temperature dependent. The absorption half-life of enrofloxacin was 1.99 h and 2.17 h after oral administration, whereas the elimination half-life of the drug was 98.63 h and 136.59 h at 16℃ and 10℃, respectively. The peak concentration of enrofloxacin in plasma and tissues was higher at 16℃ than that at 10℃, and the peak plasma concentration time in the liver was the shortest at both temperatures among those of other tissues. The plasma ℃/MIC ratio varied between 11.08 and 5 540.00 at 16℃; and between 7.92 and 3 960.00 at 10℃. The AUC/MIC ratio was 467.82-280 690.00 at 16℃, and 359.48-215 690.00 at 10℃. These ratios indicate that it is possible to obtain therapeutic efficacy. Very low levels of ciprofloxacin were detected. The AUC ratios of ciprofloxacin and enrofloxacin in plasma suggest that plasma ciprofloxacin might play a minor role in enrofloxacin treatment for turbot.

关键词： scophthalmus maximus enrofloxacin ciprofloxacin pharmacokinetics

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Morphological comparison between a selected fast-growing strain and the common cultured strain of turbot scophthalmus maximus

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Chinese Journal of Oceanology and Limnology 2012年第4期30卷 563-568页

作者：梁兴明马爱军王新安李娟黄智慧商晓梅 Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology

To evaluate the effect of genetic improvement of the turbot scophthalmus maximus, we analyzed morphological differences between a fast-growing strain obtained by family selection and the common cultured strain, by principal component analysis, stepwise discriminant analysis, and t-tests. Although they clearly differed morphologically, plots of the principal components of the two strains partially overlapped. However, the difference between the strains was supported with very high precision by discriminant analysis. The t-tests revealed that 4 of the 13 morphological traits analyzed were highly significantly different (P<0.01), 4 traits also differed significantly (P<0.05), and the remainder did not differ significantly. The coefficients of difference of the 13 traits were all lower than the threshold value between subspecies (1.28). Together, the results indicate that a trend for segregation of characters from the common cultured strain have already appeared in the selected fast-growing strain but the degree of segregation have not risen to subspecies level.

关键词： scophthalmus maximus divergence by selection quantitative trait analysis

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Establishment,Characterization and Expression Pattern of a Spleen Cell Line,SMSP,Derived from Turbot(scophthalmus maximus)

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Journal of Ocean University of China 2023年第5期22卷 1403-1411页

作者： XUE Ting LIU Yiping GE Xuefeng LI Chao School of Marine Science and Engineering Qingdao Agricultural UniversityQingdao 266109China

A turbot(scophthalmus maximus)cell line named SMSP was obtained from the *** origin of the cells was identified by morphology,chromosome number and COI *** optimal basic medium,serum concentration and growth temperature of the cells were *** cell line is mainly composed of fibroblast-like *** of the SMSP cells contained 44 chromosomes,and the sequence of COI gene confirmed that the cells were originated from *** optimal culture conditions were 24℃,DMEM+10%*** cell line had high transfection efficiency for siRNA and *** stimulation with lipopolysaccharide(LPS)or poly(I:C),the expressions of immune-related genes such as TNF-β,IL-12s,IL-10 and IL-1βwere up-regulated significantly in the early stage(P<0.05).This study will provide a model for exploring immune mechanism of turbot against pathogen in vitro.

关键词： scophthalmus maximus cell line spleen pathogen-associated molecular patterns immunity

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Genetic parameters and response to selection for body weight in turbot(scophthalmus maximus, Linnaeus)

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Acta Oceanologica Sinica 2018年第6期37卷 47-51页

作者： LYU Ding WANG Weiji LUAN Sheng HU Yulong GUAN Jiantao LI Zhixiang WU Huanhuan KONG Jie LIU Shoutang Wuxi Fisheries College Nanjing Agricultural University Wuxi 214081 China Key Laboratory for Sustainable Utilization of Marine Fisheries Resources of Ministry of Agriculture Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao 266071 China Yellow Sea Fisheries Limited Company Haiyang 265122 China

Genetic parameters and response to selection were estimated for harvest body weight in turbot. The data consisted of 10 952 individuals of 508 full-sib families from three generations（G0, G1, and G2）. The heritability estimates for G0, G1, and G2 were 0.11±0.08, 0.18±0.09, and 0.17±0.07, respectively. Over three generations, the heritability estimate was 0.19±0.04. Maternal and common environmental effects were 0.10±0.04, 0.14±0.04, and0.13±0.03 within each generation and 0.12±0.01 across generations. The selection differential in growth was 18.24 g in G0 and 21.19 g in G1 corresponding to an average of 19.72 g per generation. The genetic gains were also calculated, they were 22.06 g in G1 and 11.93 g in G2, corresponding to 6.36% and 3.52% body weight. The total genetic gain after two generations was 10.10% body weight, which indicated that the selective breeding program for the body weight trait in turbot was successful.

关键词： turbot genetic parameter selective breeding body weight scophthalmus maximus

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Transcriptome analysis reveals immune-related genes in tissues of Vibrio anguillarum-infected turbot scophthalmus maximus

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Journal of Oceanology and Limnology 2024年第1期42卷 332-344页

作者： Yuting SONG Maqsood Ahmed SOOMRO Xianzhi DONG Guobin HU College of Marine Life Sciences Ocean University of ChinaQingdao 266003China Institute of Evolution&Marine Biodiversity Ocean University of ChinaQingdao 266003China Institute of Biophysis Chinese Academy of SciencesBeijing 100101China

Turbot scophthalmus maximus is an important mariculture fish species with high economic ***,the bacterial diseases caused by Vibrio anguillarum infection bring huge economic losses to the turbot aquaculture *** understand the immune response of the turbot against *** infection and to explore novel immune-related genes,the transcriptome analysis of turbot spleen and gills were conducted after *** *** expressed genes(DEGs)were identified in spleen and gill of the turbot amounted to 17261 and 16436,respectively.A large number of immunerelated DEGs were enriched in cytokine-cytokine receptor interaction signaling pathway,and the others by the kyoto encyclopedia of genes and genomes(KEGG)*** gene ontology(GO)classification analysis revealed that *** infection had the greatest effect on biological processes and cellular *** immune-related DEGs were identified in the spleen(cstl.1,egfl6,lamb21,v2rx4,calcr,and gpr78a)and gills(ghra,sh3gl2a,cst12,inhbaa,cxcl8,and il-1b)by heat *** proteinprotein interaction(PPI)networks were constructed to analyze the immune *** results demonstrate that the maturation and antigen processing of major histocompatibility complex(MHC)class II molecule,and calcitonin-or adrenomedullin-regulated physiological activity were important events in the immunity of turbot against *** *** the gills,the protein interactions in TGF-βsignaling pathway,production of inflammatory factors,and endocytosis regulation were most *** research laid a foundation for discovering novel immune-related genes and enriching the knowledge of immune mechanisms of turbot against *** infection.

关键词： scophthalmus maximus Vibrio anguillarum transcriptome differentially expressed genes immune mechanism

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Rearing in intermediate salinity enhances immunity and disease-resistance of turbot (scophthalmus maximus L.)

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Acta Oceanologica Sinica 2011年第4期30卷 122-128页

作者： ZHANG Yanjiao MAI Kangsen MA Hongming AI Qinghui ZHANG Wenbing XU Wei Key Laboratory of Mariculture Ministry of EducationOcean University of ChinaQingdao 266003China

Studies were conducted to investigate the non-specific immune responses and disease-resistance of juvenile turbot scophthalmus maximus,cultured at four different salinities（8,20,32 and 40） . Three concentrations（3.75 × 10 7,3.75 × 10 8 and 3.75 × 10 9 CFU/ml） of Vibrio anguillarum suspension were employed at each salinity to determine the 4-day LD 50 . The serum lysozyme activity,the alternative complement pathway activity（ACH50） and the phagocytosis percentage of head kidney in turbot were tested at 24,48 and 72 h post-challenge of V. anguillarum（1.1 × 10 8 CFU/ml,0.1 ml） ,respectively,to evaluate the non-specific immune responses at the selected rearing salinities. Fish reared at salinity 20 had the lowest mortality,namely,the highest 4-day LD 50 value（8.88 ± 0.17） . Besides,the lysozyme activity,ACH50 and the phagocytosis of turbot were the highest at the salinity 20,but with the lowest at the salinity 40 treatment regardless of sampling time. In addition,the non-specific immune activities kept increasing within 72 h post-challenge of V. anguillarum,except that the lysozyme activity increased from 24 to 48 h,and then decreased from 48 to 72 h at 40 significantly. These results together indicate that rearing in intermediate salinity（20） was able to enhance the immunity and disease-resistance of turbot.

关键词： scophthalmus maximus salinity immunity disease-resistance Vibrio anguillarum

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The ontogeny of acidic digestive tract in larval turbot scophthalmus maximus based on H+/K+-ATPase and pepsinogen

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Journal of Oceanology and Limnology 2023年第3期41卷 1100-1108页

作者： Yanfeng WANG Shulan LIU Teng GUO Shihong XU Qinghua LIU Zhizhong XIAO Daoyuan MA Jun LI Liang CHI Key Laboratory of Experimental Marine Biology Centre for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdao 266071China Qingdao Agricultural University Qingdao 266109China Laboratory for Marine Biology and Biotechnology Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Southern Marine Science and Engineering Guangdong Laboratory Guangzhou 511458China Conson Oriental(Yantai)Recirculating Aquaculture Technology Co. Ltd.Yantai 264000China

Acidic digestion is an important digestive process of marine *** fish stomach,two enzymes are involved in the secretion of hydrochloric acid(HCl)and proteomic digestion:H^(+)/K^(+)-ATPase and ***,the starting of digestive function in fish is still *** reveal the details of acidic digestion of turbot scophthalmus maximus in early development,a 40 day of turbot larvae culture was *** H^(+)/K^(+)-ATPase gene from the turbot ***(smH^(+)/K^(+)-ATPase)was identified and *** on our previous discription on pepsinogen of turbot ***,we combined pepsinogen and H^(+)/K^(+)-ATPase and analyzed the mechanism of acidic digestion in *** show that the spatial and temporal expression profiles of H^(+)/K^(+)-ATPase agreed with pepsinogen A and C in turbot,indicating a synergetic action between H^(+)/K^(+)-ATPase and pepsinogen during the acidic digestion *** addition,the turbot juveniles showed a faster growth after the expressions of H^(+)/K^(+)-ATPase gene and pepsinogen gene,demonstrating that pepsin had a higher digestive efficiency,for which a compound diet should be provided to the fish from Day 22 *** study provided a reference for biology research and aquaculture of turbot and other marine fishes.

关键词： turbot scophthalmus maximus acidic digestion H+/K+-ATPase pepsinogen

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