Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot Scophthalmus maximus responded to hypo-saline seawater

作     者：CUI Wenxiao MA Aijun HUANG Zhihui WANG Xin’an SUN Zhibin LIU Zhifeng ZHANG Wei YANG Jingkun ZHANG Jinsheng QU Jiangbo CUI Wenxiao;MA Aijun;HUANG Zhihui;WANG Xin'an;SUN Zhibin;LIU Zhifeng;ZHANG Wei;YANG Jingkun;ZHANG Jinsheng;QU Jiangbo

作者机构：College of Fisheries and Life ScienceShanghai Ocean UniversityMinistry of EducationShanghai 201306China Laboratory for Marine Biology and BiotechnologyQingdao National Laboratory for Marine Science and TechnologyQingdao 266071China Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesShandong Key Laboratory of Marine Fisheries Biotechnology and Genetic BreedingQingdao Key Laboratory for Marine Fish Breeding and BiotechnologyQingdao 266071China Yantai Tianyuan Aquatic Limited CorporationYantai 264003China 

出 版 物：《Journal of Oceanology and Limnology》 (海洋湖沼学报（英文）)

年 卷 期：2020年第38卷第2期

页      面：467-479页

核心收录：

学科分类：0908[农学-水产] 09[农学] 

基　　金：Supported by the Earmarked Fund for Modern Agro-Industry Technology Research System(No.CARS-47-G01) the Ao Shan Talents Cultivation Program supported by Qingdao National Laboratory for Marine Science and Technology(No.2017ASTCP-OS04) the National Natural Science Foundation of China(No.41706168) the Agricultural Fine Breed Project of Shandong(No.2019LZGC013) the Basal Research Fund,Chinese Academy of Fishery Sciences(No.2016HY-JC0301) the Yantai Science and Technology Project(No.2018ZDCX021) 

主　　题：osmoregulation mechanisms Scophthalmus maximus hypo-saline signal transduction cell volume regulation gene expression 

摘      要：Turbot harbor a relatively remarkable ability to adapt to opposing osmotic challenges and are an excellent model species to study the physiological adaptations of flounder associated with osmoregulatory *** kidney transcriptome of turbot treated 24 h in water of hypo-salinity(salinity 5)and seawater(salinity 30)was sequenced and *** silico analysis indicated that all unigenes had significant hits in seven *** functional annotation analysis of the transcriptome showed that the immune system and biological processes associated with digestion,absorption,and metabolism played an important role in the osmoregulation of turbot in response to *** of biological processes associated with inorganic channels and transporters indicated that mineral absorption and bile secretion contributed to iono-osmoregulation resulting in cell volume regulation and cell phenotypic ***,we analyzed and predicted the mechanisms of canonical signaling *** processes involved in renin secretion,ECM-receptor interaction,adherens junction,and focal adhesion played an important role in the plasticity phenotype in hypo-stress,while the signal transduction network composed of the MAPK signaling pathway and PI3K-Akt signaling pathway with GABAergic synapse,worked in hypoosmoregulation signal transduction in the *** addition,analysis of the tissue specificity of targeted gene expression using qPCR during salinity stress was carried *** results showed that the kidney,gill,and spleen were vital regulating organs of osmotic pressure,and the osmoregulation pattern of euryhaline fish dif fered among species.