Establishment,Characterization and Expression Pattern of a Spleen Cell Line,SMSP,Derived from Turbot(Scophthalmus maximus)

作     者：XUE Ting LIU Yiping GE Xuefeng LI Chao XUE Ting;LIU Yiping;GE Xuefeng;LI Chao

作者机构：School of Marine Science and EngineeringQingdao Agricultural UniversityQingdao 266109China 

出 版 物：《中国海洋大学学报:英文版》 (Journal of Ocean University of China)

年 卷 期：2023年第22卷第5期

页      面：1403-1411页

核心收录：

学科分类：0908[农学-水产] 0707[理学-海洋科学] 09[农学] 

基　　金：the National Natural Science Foundation of China(No.31902403) the Young Experts of Taishan Scholars(No.tsqn201909130) the advanced Talents Foundation of QAU grant(No.663-1120029) the Shandong Technical System of Fish Industry(No.SDAIT-12-03) the Breeding Plan of Shandong Provincial Qingchuang Research Team(2019),China 

主　　题：Scophthalmus maximus cell line spleen pathogen-associated molecular patterns immunity 

摘      要：A turbot(Scophthalmus maximus)cell line named SMSP was obtained from the *** origin of the cells was identified by morphology,chromosome number and COI *** optimal basic medium,serum concentration and growth temperature of the cells were *** cell line is mainly composed of fibroblast-like *** of the SMSP cells contained 44 chromosomes,and the sequence of COI gene confirmed that the cells were originated from *** optimal culture conditions were 24℃,DMEM+10%*** cell line had high transfection efficiency for siRNA and *** stimulation with lipopolysaccharide(LPS)or poly(I:C),the expressions of immune-related genes such as TNF-β,IL-12s,IL-10 and IL-1βwere up-regulated significantly in the early stage(P0.05).This study will provide a model for exploring immune mechanism of turbot against pathogen in vitro.