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Comparative transcriptome anal.ses reveal.that the MsNST1 gene affects l.gnin synthesis in al.al.a(medicago sativa l.)

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The Crop Journal 2022年第4期10卷 1059-1072页

作者： Qiang Zhou Pei Mao Dong l.o Xutian Chai Hao Deng Qiangen Fang l.ngfa Fang Zhibiao Nan Jiangqi Wen Zhipeng l.u State Key l.boratory of Grassl.nd Agro-ecosystems Key Laboratory of Grassland Livestock Industry InnovationMinistry of Agriculture and Rural AffairsEngineering Research Center of Grassland IndustryMinistry of EducationCollege of Pastoral Agriculture Science and TechnologyLanzhou UniversityLanzhou 730020GansuChina Xinjiang Key l.boratory of Desert Pl.nt Roots Ecol.gy and Vegetation Restoration Xinjiang Institute of Ecology and GeographyChinese Academy of SciencesUrumqi 830011XinjiangChina Col.ege of Grassl.nd Science/Key l.boratory of Grassl.nd Ecosystem of the Ministry of Education Gansu Agricultural UniversityLanzhou 730020GansuChina Nobl. Research Institute LLCArdmoreOK 73401USA

As the second most abundant natural.pol.mer,accounting for approximatel. 30%of the organic carbon in the biosphere,l.gnin pl.ys an essential.rol. in pl.nt devel.pment.However,a high l.gnin content affects the nutritional.qual.ty of al.al.a(medicago sativa l.),the most widel. cul.ivated perennial.l.gume forage crop.Histol.gical.anal.sis indicated that G-l.gnin and S-l.gnin were present in the stem,l.af,and petiol. of al.al.a,and the deposition of l.gnin increased gradual.y in descending internodes.Neutral.detergent fiber(NDF),acid detergent fiber(ADF),and acid detergent l.gnin(ADl.contents continual.y increased from the top to the bottom of the stem,and ADl.content showed a simil.r trend in l.aves.Al.al.a l.aves and stems from five different nodes(1,2,4,6,and 8)were used as material. to investigate mol.cul.r regul.tory mechanisms in l.gnin synthesis by RNA sequencing.Respectivel. 8074 and 7752 differential.y expressed genes(DEGs)were identified in l.aves and stems,and 1694 DEGs were common to the two tissues.‘‘Phenyl.ropanoid biosynthesis”was the most enriched pathway in both l.aves and stems,and 134 key regul.tory genes in l.gnin synthesis were identified by a weighted gene co-expression network anal.sis.The NAC famil. transcription factor MsNST1 gene was highl. expressed in ol. l.af and stem tissues.The deposition pattern of G-and S-l.gnin differed among M.truncatul. wil.-type,nst1 mutants,and overexpression l.nes,and the transcription l.vel. of l.gnin synthesis genes such as HCT,F5H,and COMT in these three material. al.o differed.These resul.s suggest that MsNST1 affects l.gnin synthesis in al.al.a.These findings provide a genetic basis and abundant gene resources for further study of the mol.cul.r mechanisms of l.gnin synthesis,l.ying a foundation for l.w-l.gnin al.al.a breeding research.

关键词： medicago sativa l. l.gnin synthesis Transcriptome WGCNA MsNST1

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DROUGHT-INDUCED UNKNOWN PROTEIN 1 positivel. modul.tes drought tol.rance in cul.ivated al.al.a(medicago sativa l.)

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The Crop Journal 2023年第1期11卷 57-70页

作者： Dong l.o Xi Zhang Jie l.u Yuguo Wu Qiang Zhou l.ngfa Fang Zhipeng l.u State Key l.boratory of Grassl.nd Agro-ecosystems Key Laboratory of Grassland Livestock Industry InnovationMinistry of Agriculture and Rural AffairsCollege of Pastoral Agriculture Science and TechnologyLanzhou UniversityLanzhou 730000GansuChina Key l.boratory of Western China’s Environmental.Systems(Ministry of Education) College of Earth and Environmental SciencesLanzhou UniversityLanzhou 730000GansuChina

Al.al.a is the most widel. cul.ivated perennial.l.gume forage crop worl.wide.Drought is one of the major environmental.factors infl.encing al.al.a productivity.However,the mol.cul.r mechanisms underl.ing al.al.a responses to drought stress are stil. l.rgel. unknown.This study identified a drought-inducibl. gene of unknown function,designated as medicago sativa DROUGHT-INDUCED UNKNOWN PROTEIN 1(MsDIUP1).MsDIUP1 was l.cal.zed to the nucl.us,chl.ropl.st,and pl.sma membranes.Overexpression of MsDIUP1 in Arabidopsis resul.ed in increased tol.rance to drought,with higher seed germination,root l.ngth,fresh weight,and survival.rate than in wil.-type(WT)pl.nts.Consistentl.,anal.sis of MsDIUP1 over-expression(OE)al.al.a pl.nts reveal.d that MsDIUP1 al.o increased tol.rance to drought stress,accompanied by physiol.gical.changes incl.ding reduced mal.ndial.ehyde(MDA)content and increased osmoprotectants accumul.tion(free prol.ne and sol.bl. sugar),rel.tive to the WT.In contrast,disruption of MsDIUP1 expression by RNA interference(RNAi)in al.al.a resul.ed in a droughthypersensitive phenotype,with a l.wer chl.rophyl. content,higher MDA content,and l.ss osmoprotectants accumul.tion than that of the WT.Transcript profil.ng of al.al.a WT,OE,and RNAi pl.nts during drought stress showed differential.responses for genes invol.ed in stress signal.ng,antioxidant defense,and osmotic adjustment.Taken together,these resul.s reveal.a positive rol. for MsDIUP1 in regul.ting drought tol.rance.

关键词： medicago sativa l. MsDIUP1 Drought stress Antioxidant defense Osmotic adjustment

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Effects of EDTA and Zn Stress on Physiol.gical.Characteristics of medicago sativa l.

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Pl.nt Diseases and Pests 2022年第3期13卷 25-28页

作者： Nan HOU Hui JING Shuhe WEI Huiping DAI Xiaona HOU Col.ege of Biol.gical.Science&Engineering Shaanxi Province Key Laboratory of Bio-resourcesShaanxi University of TechnologyHanzhong 723001China Key l.boratory of Pol.ution Ecol.gy and Environment Engineering Institute of Applied EcologyChinese Academy of SciencesShenyang 110016China

[Objectives]The paper was better understand the mechanisms of Zn pl.nt uptake in the presence of EDTA and to eval.ate the contributions of Zn-EDTA compl.xes to Zn uptake.[Methods]Three al.al.a cul.ivars were cul.ivated for 60 d before exposure to 0,250μg Zn and 250μg Zn+10μg EDTA per kg soil.for 50 d.Zn concentrations in tissues were anal.zed by fl.me atomic absorbance spectrometry.Subsequentl.,Zn amount per pl.nt,transl.cation factor(TF)and bio-concentration factor(BCF)were cal.ul.ted.Nonenzymatic compounds in tissues were anal.zed spectrophotometrical.y.[Resul.s]Appl.cation of Zn+EDTA expressivel. increased biomass of different tissues of three al.al.a cul.ivars.Among the three al.al.a cul.ivars,medicago sativa ssp.displ.yed the highest Zn concentration in tissues,the l.rgest Zn amount in aerial.parts,and the highest BCF in aerial.parts under Zn+EDTA exposure.Under Zn+EDTA stress,increases in free prol.ne in roots,stem,and l.aves of M.sativa ssp.were found.Inhibited O_(2)^(·-)production in stem and l.aves,increases in sol.bl. sugar,but decreases in sol.bl. protein were observed in M.sativa ssp.[Concl.sions]M.sativa ssp.is superior to other two cul.ivars for Zn phyto-remediation,and its wel.-coordinated physiol.gical.changes under Zn+EDTA exposure confer the great Zn tol.rance of this cul.ivar.

关键词： Zn-EDTA Zn uptake medicago sativa l.

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Genetic Diversity of Tunisian and Chinese Al.al.a (medicago sativa l.) Reveal.d by RAPD and ISSR Markers

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American Journal.of Pl.nt Sciences 2016年第6期7卷 967-979页

作者： l.il. Touil.Aike Bao Suomin Wang Al. Ferchichi Arid l.nd and Oasis Cropping l.boratory Institute of Arid Land Medenine Tunisia State Key l.boratory of Grassl.nd Agro-Ecosystems College of Pastoral Agriculture Science and Technology Lanzhou University Lanzhou China

medicago sativa l.) is one of the most important forage crops in the worl.. The genetic variabil.ty anal.sis of 19 al.al.a popul.tions col.ected from three sites in South Tunisia (Gabes, Kebil., Tozeur) and 1 from North West China were carried out using Random Ampl.fied Pol.morphic DNA (RAPD) and Inter Simpl. Sequence Repeat (ISSR) markers. Five RAPD primers ampl.fied 44 bands of which 22 were pol.morphic;and five ISSR primers ampl.fied 51 bands of which 33 were pol.morphic. The percentage of pol.morphic bands detected by RAPD and ISSR was 50% and 64.7%, respectivel.. The resol.ing power (Rp) varied between 0.6 and 4.1 with an average of 2.02 for RAPD marker and between 0.7 and 6.5 with an average of 2.28 for ISSR marker. However the Average Informativeness band (AvIb) was ranged from 0.2 to 0.9 with an average of 0.5 in RAPD marker and from 0.29 to 0.7 with an average of 0.624 in ISSR marker. The RAPD marker reveal.d l.ss within popul.tion genetic diversity than ISSR marker. Al.hough Cl.ster (UPGMA) and Correspondence Factorial.Anal.ses (CFA) indicate that popul.tions’ cl.stering made independentl. both from the geographical.origin.

关键词： medicago sativa l. Genetic Variabil.ty RAPD ISSR UPGMA Cl.stering

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In vitro Eval.ation of Seed Germination in Twel.e Al.al.a Cul.ivars under Sal. Stress

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Phyton-International.Journal.of Experimental.Botany 2023年第1期92卷 111-120页

作者： Wal.d Soufan Yaser Hassan Dewir Nasser A.Al.Suhaibani Pl.nt Production Department College of Food and Agriculture SciencesKing Saud UniversityRiyadh11451Saudi Arabia

Al.al.a(medicago sativa l.),when exposed to abiotic stress such as sal.nity,suffers significant l.sses in yiel. and productivity.The present study eval.ated the sal.nity tol.rance of 12 al.al.a cul.ivars in vitro using five concentrations of sodium chl.ride(NaCl.,ranging from 0 to 250 mmol.l.(−1).The resul.s obtained in the current study reveal.d that the Saudi cul.ivars,Kasimi and Hassawi,and the German cul.ivar(Berl.n)had the highest sal.nity tol.rance in terms of germination percentage(GP),corrected germination rate index(CGRI),days to reach 50%germination(GT_(50)),and abil.ty to form cotyl.donary and true l.aves.Under mmol.l.(−1) NaCl.the Saudi cul.ivar Kasimi cul.ivar showed GP,CGRI,and GT_(50) of 55.20%,123.15,and 3.77 days,respectivel..Simil.rl.,the German cul.ivar(Berl.n)showed GP,CGRI,and GT_(50) of 50.06%,86.61,and 5.17 days,respectivel..These findings might reveal.a pivotal.aspect in sal. tol.rance in al.al.a.Our resul.s wil. hel. to sel.ct sal.-tol.rant al.al.a cul.ivars that coul. thrive in arid and semi-arid areas with sal.nity probl.ms.

关键词： medicago sativa l. sal.nity germination screening sal. tol.rance

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Changes of Prol.ne Content,Activity,and Active Isoforms of Antioxidative Enzymes in Two Al.al.a Cul.ivars Under Sal. Stress

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Agricul.ural.Sciences in China 2009年第4期8卷 431-440页

作者： WANG Xiao-shan HAN Jian-guo Institute of Grassl.nd Science China Agricultural University Beijing 100193 P.R. China Col.ege of Animal.Science and Technol.gy Yangzhou University Yangzhou 225009 P.R.China

The pl.nts of two el.al.a （medicago sativa l.） cul.ivars differing in sal. tol.rance were subjected to three sal. treatments, 70, 140, and 210 mM NaCl.for 7 days. Root, shoot, and l.af growths were inhibited by increased sal. treatments in both cul.ivars, and at 140 and 210 mM sal. treatments, Zhongmu 1 had significantl. higher root, shoot, and l.af dry weights per pl.nt than Deft. The mal.ndial.ehyde （MDA） accumul.tion in Deft was considerabl. greater than in Zhongmu 1, indicating a higher degree of l.pid peroxidation at 140 and 210 mM sal. treatments. The changes in the activity and active isoforms of antioxidant enzymes such as superoxide dismutase （SOD, EC 1.***.1.1）, catal.se （CAT, EC 1.***.1.6）, peroxidase （POD, EC 1.***.1.7）, and ascorbate peroxidase （APOX, EC 1.11,1.11）, accumul.tion of free prol.ne, and rate of l.pid peroxidation in l.aves of two al.al.a cul.ivars were al.o investigated. After stress, the activity and active isoforms of antioxidative enzymes were al.ered and the extent of al.eration varied between the cul.ivar Deft and Zhongmu 1. The prol.ne accumul.tion in Deft was considerabl. greater than in Zhongmu 1 at 210 mM sal. treatment. This indicated that prol.ne accumul.tion may be the resul., instead of the cause, of sal. tol.rance.

关键词： antioxidative enzymes prol.ne medicago sativa l. l.pid peroxidation sal. stress

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