Effects of EDTA and Zn Stress on Physiological Characteristics of Medicago sativa L.

作     者：Nan HOU Hui JING Shuhe WEI Huiping DAI Xiaona HOU 

作者机构：College of Biological Science&EngineeringShaanxi Province Key Laboratory of Bio-resourcesShaanxi University of TechnologyHanzhong 723001China Key Laboratory of Pollution Ecology and Environment EngineeringInstitute of Applied EcologyChinese Academy of SciencesShenyang 110016China 

出 版 物：《Plant Diseases and Pests》 (植物病虫害研究（英文版）)

年 卷 期：2022年第13卷第3期

页      面：25-28页

学科分类：12[管理学] 082803[工学-农业生物环境与能源工程] 0909[农学-草学] 07[理学] 08[工学] 09[农学] 1204[管理学-公共管理] 090503[农学-草业科学] 0828[工学-农业工程] 0905[农学-畜牧学] 0903[农学-农业资源与环境] 120405[管理学-土地资源管理] 0713[理学-生态学] 

基　　金：Supported by National Natural Science Foundation of China(41571300,31270540 and 31070455) Special Plan in the Major Research&Development of the 13th Five-Year Plan of China(2016YFD0800802) Open Fund of Cultivation State Key Laboratory of Qinba Biological Resources and Ecological Environment of Shaanxi University of Technology(SLGPT2019KF04-02) Scientific Research Project of City-University Co-construction of Shaanxi Province(SXJ-2101) Project of Foreign Experts Bureau of Shaanxi Province of China(2022WGZJ-20) Department of Education of Shaanxi Province(15JK1121) 

主　　题：Zn-EDTA Zn uptake Medicago sativa L. 

摘      要：[Objectives]The paper was better understand the mechanisms of Zn plant uptake in the presence of EDTA and to evaluate the contributions of Zn-EDTA complexes to Zn uptake.[Methods]Three alfalfa cultivars were cultivated for 60 d before exposure to 0,250μg Zn and 250μg Zn+10μg EDTA per kg soil for 50 *** concentrations in tissues were analyzed by flame atomic absorbance ***,Zn amount per plant,translocation factor(TF)and bio-concentration factor(BCF)were *** compounds in tissues were analyzed spectrophotometrically.[Results]Application of Zn+EDTA expressively increased biomass of different tissues of three alfalfa *** the three alfalfa cultivars,Medicago sativa *** the highest Zn concentration in tissues,the largest Zn amount in aerial parts,and the highest BCF in aerial parts under Zn+EDTA *** Zn+EDTA stress,increases in free proline in roots,stem,and leaves of *** *** *** O_(2)^(·-)production in stem and leaves,increases in soluble sugar,but decreases in soluble protein were observed in *** ssp.[Conclusions]*** *** superior to other two cultivars for Zn phyto-remediation,and its well-coordinated physiological changes under Zn+EDTA exposure confer the great Zn tolerance of this cultivar.