Comparative transcriptome analyses reveal that the MsNST1 gene affects lignin synthesis in alfalfa(Medicago sativa L.)

作     者：Qiang Zhou Pei Mao Dong Luo Xutian Chai Hao Deng Qiangen Fang Longfa Fang Zhibiao Nan Jiangqi Wen Zhipeng Liu Qiang Zhou;Pei Mao;Dong Luo;Xutian Chai;Hao Deng;Qiangen Fang;Longfa Fang;Zhibiao Nan;Jiangqi Wen;Zhipeng Liu

作者机构：State Key Laboratory of Grassland Agro-ecosystemsKey Laboratory of Grassland Livestock Industry InnovationMinistry of Agriculture and Rural AffairsEngineering Research Center of Grassland IndustryMinistry of EducationCollege of Pastoral Agriculture Science and TechnologyLanzhou UniversityLanzhou 730020GansuChina Xinjiang Key Laboratory of Desert Plant Roots Ecology and Vegetation RestorationXinjiang Institute of Ecology and GeographyChinese Academy of SciencesUrumqi 830011XinjiangChina College of Grassland Science/Key Laboratory of Grassland Ecosystem of the Ministry of EducationGansu Agricultural UniversityLanzhou 730020GansuChina Noble Research InstituteLLCArdmoreOK 73401USA 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2022年第10卷第4期

页      面：1059-1072页

核心收录：

学科分类：0710[理学-生物学] 090503[农学-草业科学] 0909[农学-草学] 0905[农学-畜牧学] 09[农学] 0901[农学-作物学] 

基　　金：supported by the National Natural Science Foundation of China(32071862 and 31722055) the China Postdoctoral Science Foundation(2020M683609) the Key Science and Technology Foundation of Gansu Province(19ZD2NA002) 

主　　题：Medicago sativa L. Lignin synthesis Transcriptome WGCNA MsNST1 

摘      要：As the second most abundant natural polymer,accounting for approximately 30%of the organic carbon in the biosphere,lignin plays an essential role in plant ***,a high lignin content affects the nutritional quality of alfalfa(Medicago sativa L.),the most widely cultivated perennial legume forage *** analysis indicated that G-lignin and S-lignin were present in the stem,leaf,and petiole of alfalfa,and the deposition of lignin increased gradually in descending *** detergent fiber(NDF),acid detergent fiber(ADF),and acid detergent lignin(ADL)contents continually increased from the top to the bottom of the stem,and ADL content showed a similar trend in *** leaves and stems from five different nodes(1,2,4,6,and 8)were used as materials to investigate molecular regulatory mechanisms in lignin synthesis by RNA *** 8074 and 7752 differentially expressed genes(DEGs)were identified in leaves and stems,and 1694 DEGs were common to the two tissues.‘‘Phenylpropanoid biosynthesiswas the most enriched pathway in both leaves and stems,and 134 key regulatory genes in lignin synthesis were identified by a weighted gene co-expression network *** NAC family transcription factor MsNST1 gene was highly expressed in old leaf and stem *** deposition pattern of G-and S-lignin differed among *** wild-type,nst1 mutants,and overexpression lines,and the transcription levels of lignin synthesis genes such as HCT,F5H,and COMT in these three materials also *** results suggest that MsNST1 affects lignin synthesis in *** findings provide a genetic basis and abundant gene resources for further study of the molecular mechanisms of lignin synthesis,laying a foundation for low-lignin alfalfa breeding research.